Check patentability & draft patents in minutes with Patsnap Eureka AI!

Anti-bacterial treatment method for anthurium transgenosis material acquired through agrobacterium-mediated transformation

An Agrobacterium-mediated and processing technology, applied to the genetic transformation technology in the biological breeding of Anthurium, the field of bacteriostatic and bacteriostatic treatment, can solve the problems such as the failure of antibiotic screening and the difficulty of effectively inhibiting the growth of Agrobacterium, and achieve no-tolerance Medicinal properties, good application value and economic benefits, and the effect of reducing Agrobacterium contamination and loss

Inactive Publication Date: 2017-11-21
SUZHOU UNIV
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problem that in the current genetic transformation of anthurium, the screening of antibiotics for the antibacterial presence of Agrobacterium contamination is ineffective, and the growth of Agrobacterium is difficult to be effectively inhibited, and provides two kinds of materials that do not affect the growth and differentiation of anthurium transgenic receptor materials, which can Antibacterial treatment method of anthurium transgenic material with broad antibacterial spectrum, strong antibacterial efficacy and long-lasting antibacterial effect to inhibit Agrobacterium contamination

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Anthurium callus was selected for subculture on 1 / 2 MS+6-BA 0.5 mg / L+2,4-D 0.1 mg / L medium, and cut into small pieces with a size of about 1 cm on each side. The callus pieces were soaked in the Agrobacterium solution for 20 min, and then inserted into the control medium (conventional medium) and containing composite nano-silver at concentrations of 25 mg / L, 30 mg / L, 45 mg / L and 50 mg, respectively. / L of solid differentiation medium (1 / 2 MS+6-BA 0.5 mg / L + 2,4-D 0.1 mg / L), the growth of Agrobacterium was investigated after culturing for 10 days.

[0020] The results showed that: in the control medium, the growth of Agrobacterium was not inhibited, and the bacterial liquid spread in the medium; in the medium containing 25 mg / L composite nano-silver antibacterial agent, although there were Agrobacterium on the surface of the callus block solution, but Agrobacterium could not grow and spread in the medium; in the medium with the concentration of 30 mg / L, 45 mg / L, and 50 m...

Embodiment 2

[0023] Select the dense green anthurium callus, cut off the surface protrusions and differentiated adventitious buds, cut into small pieces with a size of about 1 cm on each side as the transgenic recipient material, and carry out Agrobacterium infection treatment according to the conventional method, Transfer to 1 / 2 MS+6-BA0.5 mg / L+2,4-D 0.1 mg / L +Cef 500 mg / L antibacterial medium for 1 to 2 generations, transfer to 1 / 2 MS +6-BA 0.5 mg / L+2,4-D 0.1 mg / L +Hyg 20-30 mg / L +Cef 500 mg / L on the selection medium.

[0024] From the genetically modified materials screened and cultivated, take out the callus pieces that have failed to inhibit the growth of Agrobacterium and have been seriously polluted, and wash them with sterile water for 3 to 4 times to remove most of the Agrobacterium liquid adhering to the surface of the material. The anthurium callus after cleaning is transferred to the antibacterial agent solution with a composite nano-silver concentration of 25 mg / L. The composi...

Embodiment 3

[0027] Select the dense green anthurium callus, cut off the surface protrusions and differentiated adventitious buds, cut into small pieces with a size of about 1 cm on each side as the transgenic recipient material, and carry out Agrobacterium infection treatment according to the conventional method, Transfer to 1 / 2 MS+6-BA0.5 mg / L+2,4-D 0.1 mg / L +Cef 500 mg / L antibacterial medium for 1 to 2 generations, then transfer to 1 / 2 MS+6-BA 0.5 mg / L+2,4-D 0.1 mg / L +Hyg 20-30 mg / L +Cef 500 mg / L were cultured on the selection medium.

[0028] From the transgenic materials that were screened and cultivated, the callus pieces that failed to inhibit the growth of Agrobacterium were taken out, and washed with sterile water for 3 to 4 times to remove most of the Agrobacterium liquid adhered to the surface of the material. The cleaned Anthurium callus mass was transferred to the antibacterial agent solution containing 50 mg / L composite nano-silver particles, and cultured with shaking at 28 °...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to an anti-bacterial treatment method for an anthurium transgenosis material acquired through agrobacterium-mediated transformation. An anthurium callus tissue is taken as a transgenosis acceptor material and two methods are adopted for performing anti-bacterial treatment on the anthurium transgenosis material acquired through agrobacterium-mediated transformation. One method comprises the steps of cleaning with sterile water and then placing the transgenosis material into a compound nanometer silver anti-bacterial solution in concentration of 25-50mg / L and performing anti-bacterial treatment under an oscillating condition. The other method comprises the steps of directly inoculating the transgenosis material into a solid differential medium containing 25-50mg / L compound nanometer silver anti-bacterial agent and performing anti-bacterial culture. Compared with a conventional antibiotic antibacterial method, the method has the advantages that the agrobacterium pollution of the anthurium transgenosis material can be effectively restrained, the maximal antibacterial rate can reach up to 100%, the growth and differentiation of the anthurium transgenosis acceptor material are not influenced and the genetic transformation rate is obviously increased after the technical scheme according to the invention is adopted for performing compound nanometer silver anti-bacterial agent treatment and the method has ultrahigh application value and economic benefit.

Description

technical field [0001] The invention relates to a method for inhibiting and eliminating bacteria after being polluted by Agrobacterium on transgenic materials obtained by the Agrobacterium-mediated transformation method, in particular to the genetic transformation technology in anthurium biological breeding, and belongs to the field of ornamental horticulture. Background technique [0002] Anthurium ( Anthurium andraeaum ) is a perennial herbaceous flower of the genus Anthurium in the Araceae family. It is native to tropical America and is very popular among consumers because of its unique shape of flowers and leaves. With the rapid development of the anthurium flower industry, the problems existing in the anthurium production have gradually attracted people's attention. Among them, bacterial diseases have become the biological stress factors for the stable development of the anthurium industry, and the use of transgenic technology to cultivate disease-resistant varieties is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/82A01H5/00A61L2/18A61L2/02A61L101/02
CPCA61L2/02A61L2/18C12N15/8205
Inventor 管华孔周阳谈建中赵川乐李秀秀沈成晨高乐李坚
Owner SUZHOU UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com