Method for regulating and controlling accumulation of astaxanthin
A technology for astaxanthin and urea is applied in the field of regulating and controlling the accumulation of astaxanthin, which can solve the problems of high technical difficulty in production, limited output of astaxanthin, inability to meet market demands, etc. Effect
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[0022] Example 1
[0023] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:
[0024] (1) Inoculate the Haematococcus pluvialis liquid into 500ml liquid culture medium according to 15% of the inoculation amount, then place the inoculated culture medium in the photosynthetic reactor, and then pass the volume concentration of 8 into the photosynthetic reactor. % High-concentration carbon dioxide gas, cultured for 4 days under the conditions of gas flow rate of 50ml / min, light intensity of 3000 Lux, and temperature of 25°C;
[0025] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 15%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of a gas flow rate of 60ml / min, a light intensity of 3000 Lux, and a temperature of 25°C, the target algae solution is obtained by c...
Example Embodiment
[0029] Example 2
[0030] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:
[0031] (1) Inoculate chlorella liquid into 500ml liquid culture medium according to the inoculation amount of 18%, then put the inoculated culture medium in the photosynthetic reactor, and then pass the 8% volume concentration into the photosynthetic reactor. High-concentration carbon dioxide gas, cultured for 4.5 days under the conditions of gas flow rate of 55ml / min, light intensity of 3100 Lux and temperature of 26℃;
[0032] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 18%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of a gas flow rate of 65ml / min, a light intensity of 3100 Lux, and a temperature of 26°C, the target algae liquid was obtained by culturing for 4.5 days; th...
Example Embodiment
[0036] Example 3
[0037] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:
[0038] (1) Inoculate chlorella liquid into 500ml liquid culture medium according to 20% of the inoculum, then place the inoculated culture medium in the photosynthetic reactor, and then pass the 8% volume concentration into the photosynthetic reactor. High-concentration carbon dioxide gas, cultured for 5 days under the conditions of gas flow rate of 60ml / min, light intensity of 3200 Lux, and temperature of 28°C;
[0039] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 20%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of gas flow rate of 70ml / min, light intensity of 3200 Lux, and temperature of 28°C, the target algae liquid was obtained by culturing for 5 days; the composition of...
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