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Method for regulating and controlling accumulation of astaxanthin

A technology for astaxanthin and urea is applied in the field of regulating and controlling the accumulation of astaxanthin, which can solve the problems of high technical difficulty in production, limited output of astaxanthin, inability to meet market demands, etc. Effect

Inactive Publication Date: 2017-11-21
SHENZHEN DEHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high difficulty of production technology and the limitation of bacteria and algae, the output of astaxanthin is limited, which is far from meeting the market demand, which also restricts the wide application of natural astaxanthin to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for regulating astaxanthin accumulation, the specific steps are as follows:

[0024] (1) Haematococcus pluvialis liquid is inoculated in the 500ml liquid culture medium by 15% inoculum size, then the culture medium after the inoculation is placed in the photosynthetic reactor, then passes into the photosynthetic reactor with a volume concentration of 8 % high-concentration carbon dioxide gas, cultivated for 4 days under the conditions of gas flow rate of 50ml / min, light intensity of 3000Lux, and temperature of 25°C;

[0025] (2) The above-mentioned algae strain after being cultured with 8% carbon dioxide with a volume concentration is transferred into a new liquid medium with an inoculum size of 15%, and then a high-concentration carbon dioxide gas with a volume concentration of 12% is passed into it. Under the conditions of gas flow rate of 60ml / min, light intensity of 3000Lux, and temperature of 25°C, the target algae liquid was obtained by culturing for 4 da...

Embodiment 2

[0030] A method for regulating astaxanthin accumulation, the specific steps are as follows:

[0031] (1) Chlorella liquid is inoculated into 500ml liquid culture medium by 18% inoculum size, then the culture medium after inoculation is placed in the photosynthetic reactor, then in the photosynthetic reactor, pass into the photosynthetic reactor with a volume concentration of 8% High-concentration carbon dioxide gas, cultivated for 4.5 days under the conditions of gas flow rate of 55ml / min, light intensity of 3100Lux, and temperature of 26°C;

[0032] (2) The above-mentioned algae strain after the carbon dioxide culture with the volume concentration of 8% is transferred into a new liquid medium with an inoculum size of 18%, and then the high-concentration carbon dioxide gas with a volume concentration of 12% is passed into it, and the Under the conditions of gas flow rate of 65ml / min, light intensity of 3100Lux, and temperature of 26°C, the target algae liquid was obtained by c...

Embodiment 3

[0037] A method for regulating astaxanthin accumulation, the specific steps are as follows:

[0038] (1) Chlorella liquid is inoculated into 500ml liquid culture medium by 20% inoculum size, then the culture medium after inoculation is placed in the photosynthetic reactor, then passes into the photosynthetic reactor with a volume concentration of 8% High-concentration carbon dioxide gas, cultivated for 5 days under the conditions of gas flow rate of 60ml / min, light intensity of 3200Lux, and temperature of 28°C;

[0039] (2) The above-mentioned algae strain after the carbon dioxide culture with the volume concentration of 8% is transferred into a new liquid medium with a 20% inoculum size, and then the high-concentration carbon dioxide gas with a volume concentration of 12% is introduced, and the Under the conditions of gas flow rate of 70ml / min, light intensity of 3200Lux, and temperature of 28°C, the target algae liquid was obtained by culturing for 5 days; the composition of...

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PUM

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Abstract

The invention discloses a method for regulating and controlling accumulation of astaxanthin. The method includes domesticating haematococcus pluvialis strains by the aid of carbon dioxide in procedures for culturing the haematococcus pluvialis strains, adding jasmonic acid mother liquor, an inducer, plant hormones and iron ions into solution and carrying out shaking fermentation culture under the culture conditions of the temperatures of 20-30 DEG C, the light intensity of 0-200 micro-mol.m<2>.s<1>, the rotational speeds of 100-200 rpm and the light-dark ratios of 12-24 h / 12-24 h. The method has the advantages that iodine salt is used as the inducer, and accordingly transformation from nutrient cells of haematococcus pluvialis to chlamydospores can be effectively promoted; sufficient carbon sources can be provided by the high-concentration carbon dioxide in flue gas for synthesizing oil in astaxanthin synthesis phases, favorable conditions can be provided for esterifying and storing the astaxanthin, accumulation of the astaxanthin in the haematococcus pluvialis can be obviously accelerated, the production cost of the astaxanthin can be reduced, and the method is simple and feasible and is low in cost, high in efficiency and suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for regulating astaxanthin accumulation. Background technique [0002] Astaxanthin is a bright red fat-soluble pigment that belongs to the group of ketocarotenoids. A large number of experiments have shown that the most significant activities of astaxanthin include its antioxidant properties and anti-inflammatory properties, which can improve immunity, prevent the occurrence and development of chronic diseases such as tumors, and have physiological and biological effects of delaying aging. It is used in food additives, Cosmetics, health care products and pharmaceutical industries have broad application prospects. For decades, the production of natural astaxanthin by various methods has been a hot issue that has been widely concerned at home and abroad. Astaxanthin has broad application prospects in aquaculture and poultry farming, food, cosmetics, medicine and ot...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12N1/12C12R1/89
CPCC12N1/12C12P23/00
Inventor 蔡明翰
Owner SHENZHEN DEHE BIOTECH CO LTD
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