Method for regulating and controlling accumulation of astaxanthin

A technology for astaxanthin and urea is applied in the field of regulating and controlling the accumulation of astaxanthin, which can solve the problems of high technical difficulty in production, limited output of astaxanthin, inability to meet market demands, etc. Effect

Inactive Publication Date: 2017-11-21
SHENZHEN DEHE BIOTECH CO LTD
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high difficulty of production technology and the limitation of bacteria and algae, the output of astaxanthin is limited,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:

[0024] (1) Inoculate the Haematococcus pluvialis liquid into 500ml liquid culture medium according to 15% of the inoculation amount, then place the inoculated culture medium in the photosynthetic reactor, and then pass the volume concentration of 8 into the photosynthetic reactor. % High-concentration carbon dioxide gas, cultured for 4 days under the conditions of gas flow rate of 50ml / min, light intensity of 3000 Lux, and temperature of 25°C;

[0025] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 15%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of a gas flow rate of 60ml / min, a light intensity of 3000 Lux, and a temperature of 25°C, the target algae solution is obtained by c...

Example Embodiment

[0029] Example 2

[0030] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:

[0031] (1) Inoculate chlorella liquid into 500ml liquid culture medium according to the inoculation amount of 18%, then put the inoculated culture medium in the photosynthetic reactor, and then pass the 8% volume concentration into the photosynthetic reactor. High-concentration carbon dioxide gas, cultured for 4.5 days under the conditions of gas flow rate of 55ml / min, light intensity of 3100 Lux and temperature of 26℃;

[0032] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 18%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of a gas flow rate of 65ml / min, a light intensity of 3100 Lux, and a temperature of 26°C, the target algae liquid was obtained by culturing for 4.5 days; th...

Example Embodiment

[0036] Example 3

[0037] A method for regulating the accumulation of astaxanthin, the specific steps are as follows:

[0038] (1) Inoculate chlorella liquid into 500ml liquid culture medium according to 20% of the inoculum, then place the inoculated culture medium in the photosynthetic reactor, and then pass the 8% volume concentration into the photosynthetic reactor. High-concentration carbon dioxide gas, cultured for 5 days under the conditions of gas flow rate of 60ml / min, light intensity of 3200 Lux, and temperature of 28°C;

[0039] (2) The above-mentioned algae strains cultured with carbon dioxide with a volume concentration of 8% are transferred to a new liquid medium with an inoculum of 20%, and then a high concentration of carbon dioxide gas with a volume concentration of 12% is introduced. Under the conditions of gas flow rate of 70ml / min, light intensity of 3200 Lux, and temperature of 28°C, the target algae liquid was obtained by culturing for 5 days; the composition of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for regulating and controlling accumulation of astaxanthin. The method includes domesticating haematococcus pluvialis strains by the aid of carbon dioxide in procedures for culturing the haematococcus pluvialis strains, adding jasmonic acid mother liquor, an inducer, plant hormones and iron ions into solution and carrying out shaking fermentation culture under the culture conditions of the temperatures of 20-30 DEG C, the light intensity of 0-200 micro-mol.m<2>.s<1>, the rotational speeds of 100-200 rpm and the light-dark ratios of 12-24 h/12-24 h. The method has the advantages that iodine salt is used as the inducer, and accordingly transformation from nutrient cells of haematococcus pluvialis to chlamydospores can be effectively promoted; sufficient carbon sources can be provided by the high-concentration carbon dioxide in flue gas for synthesizing oil in astaxanthin synthesis phases, favorable conditions can be provided for esterifying and storing the astaxanthin, accumulation of the astaxanthin in the haematococcus pluvialis can be obviously accelerated, the production cost of the astaxanthin can be reduced, and the method is simple and feasible and is low in cost, high in efficiency and suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for regulating astaxanthin accumulation. Background technique [0002] Astaxanthin is a bright red fat-soluble pigment that belongs to the group of ketocarotenoids. A large number of experiments have shown that the most significant activities of astaxanthin include its antioxidant properties and anti-inflammatory properties, which can improve immunity, prevent the occurrence and development of chronic diseases such as tumors, and have physiological and biological effects of delaying aging. It is used in food additives, Cosmetics, health care products and pharmaceutical industries have broad application prospects. For decades, the production of natural astaxanthin by various methods has been a hot issue that has been widely concerned at home and abroad. Astaxanthin has broad application prospects in aquaculture and poultry farming, food, cosmetics, medicine and ot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P23/00C12N1/12C12R1/89
CPCC12N1/12C12P23/00
Inventor 蔡明翰
Owner SHENZHEN DEHE BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products