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Non-purified nucleic acid amplification method and device

A nucleic acid amplification device and nucleic acid technology, applied in the field of molecular biology, can solve problems such as time-consuming, increased difficulty and cost of use, unreliable test results, etc.

Active Publication Date: 2017-11-24
ZHUHAI ASTROBIO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purification process of nucleic acid requires the use of additional consumables and instruments, which is not only time-consuming, but also increases the difficulty and cost of use
Moreover, when using existing purification methods to purify nucleic acids from a small amount of samples, the recovery rate of nucleic acids is low, which easily leads to unreliable detection results

Method used

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  • Non-purified nucleic acid amplification method and device
  • Non-purified nucleic acid amplification method and device
  • Non-purified nucleic acid amplification method and device

Examples

Experimental program
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no. 1 example

[0036] The device used in this embodiment is as Figures 1 to 2 As shown, it includes a sampler 11 and a reaction device 12 .

[0037] Wherein, the sampler 11 includes a sealing block 111 and a sample needle 112 fixedly connected with the sealing block 111 . The sealing block 111 takes the form of a plug. The sample needle 112 is made of metal with a diameter between 0.1 and 5 mm and a ratio of length to diameter greater than or equal to 3. The radial surface of the lower end of the sample needle 112 is a rough surface, forming a hydrophilic surface 113 . In this embodiment, the sampler 11 further includes a protective cover 114 , before the sample needle 112 is used, the sample needle 112 is inserted into the protective cover 114 for protection to prevent the sample needle 112 from being contaminated.

[0038] The reaction device 12 may include a reaction container 121 and a cover body 122, and the reaction container 121 and the cover body 122 form a hermetic whole. The c...

no. 2 example

[0043] The device used in this embodiment is as Figures 3 to 4 As shown, it includes a sampler 21 and a reaction device 22 . The sampler 21 includes a sealing block 211 , a sample needle 212 and a protective cover 214 , and the lower end of the sample needle 212 is provided with a hydrophilic surface 213 . The sample needle 212 penetrates the sealing block 211 and can move up and down along the sealing block 211 , and a pull block 215 is provided at the upper end of the sample needle 212 for lifting the sample needle 212 upward. The rest of the structure of the device is the same as that of the first embodiment.

[0044] When the device of this embodiment is used for the amplification reaction of unpurified nucleic acid, the steps are basically the same as those of the first embodiment. The difference is that if Figure 4 As shown, after adding the sample, the pull block 215 is moved to drive the sample needle 212 to move upward, so that the sample needle 212 is separated ...

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Abstract

The invention relates to a non-purified nucleic acid amplification method and device. The method comprises the following steps of providing a reaction device, and placing an amplification reaction reagent in the reaction device; using a sampler for sampling, wherein the sampler comprises a sealing block and a sample probe connected with the sealing block, a hydrophilic surface is arranged at the tail end of the sample probe, and the sample probe contacts a non-purified nucleic acid sample through the hydrophilic surface so as to sample; inserting the hydrophilic surface into the amplification reaction reagent, and sealing the reaction device through the sealing block; controlling the temperature of the reaction device through a temperature controller, and carrying out amplification reaction. The device provided by the invention is used for implementing the method. According to the non-purified nucleic acid amplification method and device provided by the invention, the processes of sample preparation and purification can be avoided, the non-purified sample is directly amplified, the operation difficulty is reduced, the time is saved, and the cost is reduced.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for qualitative or quantitative determination of nucleic acid. Background technique [0002] With the development of biotechnology, modern molecular biology technology or genetic engineering technology is being increasingly widely used in various biotechnology industries, especially in medical diagnosis. The application of this kind of technology often involves qualitative and quantitative determination with molecular probes. For example, when the nucleic acid (DNA or RNA) samples of animals and plants are determined, the nucleic acid in the sample needs to be purified first, and then the nucleic acid amplification reaction is performed. Typical nucleic acid amplification reactions are polymerase chain reaction (PCR) or reverse transcription-polymerase chain reaction (RT-PCR). [0003] However, the purification of nucleic acid is a complicated process. For different sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12M1/38C12M1/26C12M1/24
CPCC12M23/06C12M33/04C12M41/12C12Q1/6806B01L2300/0832B01L2200/0642B01L3/5029B01L3/50853B01L3/50851B01L2300/0829B01L2300/165B01L2400/0406B01L2400/088B01L3/508B01L3/565B01L2200/16B01L2300/041B01L2300/06B01L2300/161C12P19/34
Inventor 苏星吴开原
Owner ZHUHAI ASTROBIO BIOTECH CO LTD