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Two-photon fluorescence immunochromatographic kit for quantitative determination of pro-gastrin-releasing peptide (pro-GRP) and preparation method thereof

A technology of gastrin-releasing peptide and fluorescent immunochromatography, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of unsatisfactory primary hospitals, long response time, complicated operation, etc., and achieve the reduction of non-specific adsorption and non-specific The effect of low opposite sex and high luminous intensity

Inactive Publication Date: 2017-11-24
重庆康巨全弘生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The Chinese invention patent application with the application number CN201510474660.8 discloses a preparation method of a gastrin-releasing peptide precursor (Pro-GRP) rapid diagnostic kit, which is mainly based on microtiter plates (such as 96-well plates, 384-well plates) For analysis, the sensitivity is high, but the operation is complicated, the reaction time is long, and the cost is high
And if the sample size is relatively small, you need to wait, which cannot meet the needs of primary hospitals, including county-level and township hospitals and clinics.

Method used

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  • Two-photon fluorescence immunochromatographic kit for quantitative determination of pro-gastrin-releasing peptide (pro-GRP) and preparation method thereof
  • Two-photon fluorescence immunochromatographic kit for quantitative determination of pro-gastrin-releasing peptide (pro-GRP) and preparation method thereof
  • Two-photon fluorescence immunochromatographic kit for quantitative determination of pro-gastrin-releasing peptide (pro-GRP) and preparation method thereof

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Embodiment 1

[0040] Example 1: Modified antibodies with fluorescent dyes in a covalent cross-linking manner, and quantitative detection of gastrin-releasing peptide precursor (pro-GRP) by Wash immunochromatography

[0041] 1) Coupling of fluorescent dyes and antibodies

[0042] Mix a fluorescent dye with an emission wavelength of 365nm and 1 mg / ml gastrin-releasing peptide precursor (pro-GRP) monoclonal antibody, react at room temperature for 3 hours, add 1mol / L hydroxylamine hydrochloride to terminate the reaction, and use a chromatographic column or chromatography Column separation and purification were performed to obtain monoclonal antibody to gastrin-releasing peptide precursor (pro-GRP) modified by fluorescent dye. Similarly, fluorescent dye-modified rabbit IgG (quality control molecule) was obtained. Wherein the fluorescent emission wavelength of the fluorescent dye modified for the gastrin releasing peptide precursor (pro-GRP) antibody and that of the fluorescent dye modified rabb...

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Abstract

The invention discloses a two-photon fluorescence immunochromatographic kit using a fluorescent dye as a marker and used for quantitative determination of pro-GRP. The two-photon fluorescence immunochromatographic kit provided by the invention realizes fluorescent quantitative detection of pro-GRP, has the advantages of good stability, a wide linear range, good specificity, accurate quantification, easiness and quickness, can simultaneously detect whole blood, serum and plasma samples, and is applicable to all levels of hospitals and family practice.

Description

technical field [0001] The invention relates to the field of cancer clinical immunodiagnosis kits in the field of medical testing, in particular to a two-photon fluorescence immunochromatography kit for quantitatively detecting gastrin releasing peptide precursor (pro-GRP). Background technique [0002] Pro-GRP is the precursor structure of gastrin releasing peptide (GRP). GRP and Pro-GRP play an important role in the occurrence, development and treatment of tumors. Pro-GRP shows the level of GRP and the expression of GRP gene. It is a new tumor marker of SCLC and has been widely used at home and abroad. Since SCLC has the nature of neuroendocrine tumors, Pro-GRP and NSE have good diagnostic value in the diagnosis of SCLC. [0003] A study compared the differences in the levels of Pro-GRP and NSE in the serum of healthy people, patients with benign lung diseases, NSCLC patients, and SCLC patients, analyzed the application of the two in the diagnosis of SCLC, and initially ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/533
CPCG01N33/558G01N33/533
Inventor 李道锋张铁汉段德民潘文东
Owner 重庆康巨全弘生物科技有限公司
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