High-flux single-cell whole genome amplification method

A whole-genome amplification, single-cell technology, applied in the field of high-throughput single-cell whole-genome amplification, can solve the problems of poor fidelity, non-specific amplification, poor repeatability, etc., and achieve the effect of automation and scale
CN107400705AInactive Publication Date: 2017-11-28MGI TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MGI TECH CO LTD
Publication Date
2017-11-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a high-flux single-cell whole genome amplification method. The method comprises the steps as follows: a cell suspension with preset cell density is distributed to each micropore of a nano microporous chip by use of a nano micro liquid distribution platform; a cell lysis buffer is distributed to micropores by use to the micro liquid distribution platform to lyse cells in the micropores; a neutralization solution is distributed to micropores by use to the micro liquid distribution platform for a neutralization reaction; a whole genome amplification reagent is distributed to micropores by use to the micro liquid distribution platform for a whole genome amplification reaction. The method has the characteristics of being high in flux, low in cost, single-cell and integrated, and automatic and large-scale single-cell whole genome amplification can be realized.
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Description

technical field

[0001] The invention relates to the technical field of single-cell whole-genome amplification, in particular to a high-throughput single-cell whole-genome amplification method. Background technique

[0002] Studies have shown that in the process of embryonic development, disease occurrence, and tumor development, due to the individual differences and heterogeneity of the genetic material of a single cell, it leads to extremely important or even decisive consequences. Therefore, it is necessary to sequence the genome of a single cell of. At present, single-cell genome sequencing has been successfully applied to various microbial and mammalian cells.

[0003] In single-cell research work, scaling up experiments is key to ensuring that sufficient biodiversity information is collected. Although single cells can be separated and samples can be amplified using microfluidic chips, the throughput is not high. Other methods for single cells have many defects, resul...

Claims

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