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A method for detecting metal ions based on hybridization chain reaction signal amplification technology

A technology of hybridization chain reaction and technical detection, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, electrochemical variables of materials, etc., can solve problems such as threats to aquatic organisms and human health, and achieve high selectivity and resistance Interference ability, enhanced selectivity, wide linear range effect

Inactive Publication Date: 2019-07-09
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there must be some silver-containing residues discharged into the water environment, which poses a potential threat to aquatic organisms and human health.

Method used

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  • A method for detecting metal ions based on hybridization chain reaction signal amplification technology
  • A method for detecting metal ions based on hybridization chain reaction signal amplification technology
  • A method for detecting metal ions based on hybridization chain reaction signal amplification technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A combined detection of heavy metal Ag based on magnetic nanoparticles and hybridization chain reaction amplification technology+ ionic method

[0034] The invention is based on the combined use of magnetic nanoparticles and hybrid chain reaction amplification technology to detect heavy metal Ag + The principle of the ionic method is as follows figure 1 shown. C-base-rich nucleic acid strand S1 is fixed to Fe via Au-S bond 3 o 4 @Au core-shell magnetic nanoparticle surface, on the target silver ion Ag + In the presence of ferrocene-labeled another C base-rich nucleic acid strand S2 passes through the C-Ag + The -C structure forms double-stranded DNA with S1 on the surface of the magnetic particle. When ferrocene-labeled hairpin DNA H1 and H2 are added, the hairpin-shaped H1 and H2 are opened under the induction of S2 and hybridization chain reaction occurs on the surface of magnetic nanoparticles, and the formed long-chain DNA complex passes through The ...

Embodiment 2

[0035] Embodiment 2 Oligonucleotide sequence design

[0036] The oligonucleotide sequence designed in the present invention was synthesized by China Shanghai Sangon Bioengineering Co., Ltd., purified and tested by HPLC, and freeze-dried. The oligonucleotide sequence designed by the present invention is as follows:

[0037] S1: 5'-SH-CAC TTC TCT CTT CTC TTC CCT CTC-3';

[0038] S2: 5’-AGG AGT AGA CTA GAT CGG ACA CAC ACC CAA CAC AAC ACA CAA CTC-NH 2 -3';

[0039] H1: 5’-TGT CCG ATC TAG TCT ACT CCT ACT GTG AGG AGT AGA CAT GAT-NH 2 -3';

[0040] H2: 5’-AGG AGT AGA CTA GAT CGG ACA ATC TAG TCT ACT CCT CAC AGT-NH 2 -3';

[0041] The oligonucleotides were dissolved in ultrapure sterile water and stored at -18°C for later use.

Embodiment 3

[0042] Example 3 Fe 3 o 4 Preparation of @Au core-shell magnetic nanoparticles

[0043] Take 1 g FeCl 2 4H 2 O, 2.6 g FeCl 3 6H 2 O and 0.425 mL of concentrated HCl were dissolved in 100 mL of water, and ultrasonically deoxygenated. Add the above mixture dropwise to 125 mL 0.75 M NaOH solution, N 2 Stir at 80°C under protection to get Fe 3 o 4 magnetic nanoparticles. Prepare 5 g / L of Fe 3 o 4 Ethanol solution 25 mL, ultrasonically dispersed, dropwise added 0.4 mL 3-aminopropyltriethoxysilane, stirred at room temperature for 7 h, diluted to 1 g / L, added 14 mL 0.6 M HAuCl 4 3H 2 O and 0.3 mL of 0.2 M sodium citrate solution, sonicated at room temperature, the color of the solution changed from light yellow to black, and Fe 3 o 4 @Au core-shell magnetic nanoparticles, magnetic separation and washing, dry storage.

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Abstract

The invention relates to a method for detecting metal ions based on a hybrid chain type reaction signal amplification technique, belonging to the technical field of analytical chemistry or environmental monitoring. The method comprises the following steps: fixing a nucleic acid chain S1 rich in C basic groups to the surfaces of gold-packaged magnetic nanoparticles through Au-S bonds by virtue of a self-assembling technique; in the presence of silver ions (Ag<+>), forming double-stranded DNA by a ferrocene labeled nucleic acid chain S2 rich in C basic groups and the nucleic acid chain S1 by virtue of a C-Ag<+>-C structure; after adding ferrocene labeled hairpin structure DNA H1 and H2, opening the hairpin structures H1 and H2 under the induction of S2, generating hybrid chain type reaction on the surfaces of the magnetic nanoparticles, and carrying out magnetic enrichment on generated compounds on the surfaces of the magnetic nanoparticles, so as to realize the amplification of electrochemical response signals. By determining the concentration of Ag<+> in a solution by virtue of the enhancement of electrochemical signals, the method has the characteristics of high sensitivity and selectivity, simplicity, rapidness and the like.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry or environmental monitoring, and in particular relates to an electrochemical method for detecting heavy metal silver ions based on magnetic nanoparticles and hybridization chain reaction signal amplification technology. Background technique [0002] As a precious metal, silver has excellent optical, electrical and bactericidal properties, so it is widely used in various industries such as energy, photography, and medicine. Therefore, there must be some silver-containing residues discharged into the water environment, which poses a potential threat to aquatic organisms and human health. Silver ions (Ag + ) can enter the human body through the food chain or drinking water, resulting in the inactivation of sulfur-containing enzymes in the body, and can also combine with various metabolites such as amines and imidazoles to cause various diseases, such as gastroenteritis, nervous disorder...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/682
CPCG01N27/48
Inventor 张艳丽李海燕庞鹏飞王红斌
Owner YUNNAN MINZU UNIV
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