Application of lactobacillus plantarum in preventing intestinal barrier injury
A technology of Lactobacillus plantarum and intestinal barrier, applied in the biological field, can solve the problem of no specific medicine for intestinal barrier
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[0073] Example 1. Isolation, identification and preservation of strains
[0074] 1. Separation of strains
[0075] Sample taken: traditional fermented sauerkraut.
[0076] Streak culture was repeated on the BCP medium plate, and the single colony that produced yellow circles was picked and inoculated on the MRS medium plate for continued cultivation. After repeated streak culture and purification on the MRS medium plate, multiple pure culture strains were obtained.
[0077] The purely cultured strains are inoculated into liquid MRS medium and cultured, then 20% glycerol is added and stored in a refrigerator at -80°C.
[0078] Positive Gram stain, negative catalase test, negative indole reaction, negative hydrogen sulfide production test, negative gelatin liquefaction test, negative starch hydrolysis test, and negative nitrate reduction test. Many strains were selected from pure culture Strain Lactobacillus. One of the strains of Lactobacillus plantarum was named Sc52.
[0079] 2. Ident...
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[0087] Example 2. Application of Lactobacillus plantarum Sc52 in improving the intestinal barrier
[0088] 1. Preparation of bacterial suspension
[0089] Take the bacteria of Lactobacillus plantarum Sc52 and suspend them in sterile saline to obtain a bacterial concentration of 1×10 10 CFU / mL bacterial suspension A.
[0090] Take the cell of Lactobacillus plantarum CICC-6009 and suspend it in sterile saline to obtain a bacterial concentration of 1×10 10 CFU / mL bacterial suspension B.
[0091] Two, group processing
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