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A kind of preparation method and application of calcium phosphate/gelatin composite nanoparticle

A composite material and nanoparticle technology, applied in the field of bioengineering, can solve the problems of large specific surface area and poor mechanical properties of colloidal composite materials, and achieve the effects of stable reaction conditions, biocompatibility, and increased production

Active Publication Date: 2020-03-31
SHENZHEN HUA NOVA BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these composite microspheres are all micron-scale particles with large specific surface area. It is difficult to form a viscous colloidal network with sufficient mechanical support due to the limited interaction between particles when using colloidal materials stacked with micron particles. Colloidal composites with poor mechanical properties

Method used

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  • A kind of preparation method and application of calcium phosphate/gelatin composite nanoparticle
  • A kind of preparation method and application of calcium phosphate/gelatin composite nanoparticle
  • A kind of preparation method and application of calcium phosphate/gelatin composite nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Calcium phosphate / gelatin composite nanoparticles were prepared as follows:

[0066] (1) Dissolve 1.25g of gelatin in 25mL of deionized water, and keep heating to 40°C to obtain a clear and transparent gelatin aqueous solution; dissolve 0.138g of disodium hydrogen phosphate in the gelatin aqueous solution, and continue stirring and heating at 300rpm At 40°C, add NaOH solution dropwise to adjust the pH value of the solution to 10 to obtain disodium hydrogen phosphate-gelatin aqueous solution;

[0067](2) Dissolve 0.106g of calcium chloride in 75mL of absolute ethanol, and after complete dissolution, add the calcium chloride ethanol solution drop by drop into the above-mentioned disodium hydrogen phosphate gelatin aqueous solution, and keep heating at 40°C and continue stirring (1000rpm), along with dropping process carries out, forms the suspension of calcium phosphate / gelatin composite material nanoparticle gradually, theoretical calculation, the calcium / phosphorus rati...

Embodiment 2

[0091] Calcium phosphate / gelatin composite nanoparticles were prepared as follows:

[0092] (1) Dissolve 1.25g of gelatin in 25mL of deionized water at 30°C, 40°C and 60°C respectively, and keep the temperature to obtain a clear and transparent gelatin aqueous solution; dissolve 0.138g of disodium hydrogen phosphate in the gelatin aqueous solution In, and keep stirring (300rpm) and heating, the pH value of above-mentioned aqueous solution is adjusted to 10 by adding NaOH solution dropwise, obtains the gelatin aqueous solution of disodium hydrogen phosphate;

[0093] (2) Dissolve 0.106g of calcium chloride in 75mL of absolute ethanol, and after the complete dissolution, add the calcium chloride ethanol solution drop by drop into the above-mentioned disodium hydrogen phosphate gelatin aqueous solution, and respectively 30 ℃, 40 ℃ and 60°C and continuous stirring (500-1000rpm), as the dropping process proceeds, a suspension of calcium phosphate / gelatin composite nanoparticles is ...

Embodiment 3

[0100] Calcium phosphate / gelatin composite nanoparticles were prepared as follows:

[0101] (1) Dissolve 1.25g of gelatin in 25mL of deionized water, and keep the temperature to obtain a clear and transparent gelatin aqueous solution; dissolve 0.138g of disodium hydrogen phosphate in the gelatin aqueous solution, and keep stirring (300rpm) and heating, by dropping Add NaOH solution to adjust the pH value of the above aqueous solution to 10 to obtain a gelatin aqueous solution of disodium hydrogen phosphate;

[0102] (2) Dissolve 0.106g of calcium chloride in dehydrated alcohol of 50, 75, 125 and 225mL respectively, and add the calcium chloride ethanol solution dropwise to the hydrogen phosphate obtained in step (1) after the complete dissolution In the gelatin aqueous solution of disodium, keep stirring (500-1000rpm) under the heating condition of 40 ℃, along with the dropwise addition process, gradually form the suspension of calcium phosphate / gelatin composite nanoparticle, ...

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Abstract

The invention provides a preparation method of calcium phosphate / gelatin compound material nano particles and application thereof. The preparation method comprises, adding phosphate into gelatin aqueous solution to obtain phosphate-gelatin aqueous solution; dissolving calcium salt into polar organic solvent, dropwise adding the solution into the phosphate-gelatin aqueous solution to obtain calcium phosphate / gelatin compound material nano particle suspension liquid; keeping the temperature below 20-90 DEG C, adding cross-linking agent into the suspension liquid for cross-linking, performing repeated centrifugal treatment and resuspension in deionized water on the reaction mixture to obtain the calcium phosphate / gelatin compound material nano particles. The invention first provides the coprecipitation preparation method of the calcium phosphate / gelatin compound material nano particles, achieves one-step preparation of the calcium phosphate / gelatin compound material nano particles and lays foundation for industrial scale production.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a preparation method and application of calcium phosphate / gelatin composite material nanoparticles. Background technique [0002] As the problem of population aging continues to intensify, the repair and reconstruction of defective or diseased tissues has become an important scientific proposition in the field of biomedicine. Bone tissue is one of the most important tissues in the human body. At present, the common treatment measures for the repair of human bone tissue in clinical practice are still using autologous bone and allogeneic bone to fill and repair bone defects. This treatment method has major disadvantages, such as the lack of donors, and it is easy to cause infection and rejection of the original tissue. These limit the wide application of this method and encourage scientific researchers to explore and develop new methods and materials. Therefore, bone tissue engineerin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/54A61L27/22A61L27/12
CPCA61L27/12A61L27/222A61L27/50A61L27/54A61L2300/414A61L2400/06A61L2430/02
Inventor 王华楠
Owner SHENZHEN HUA NOVA BIOTECH LTD
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