Production technology of compound microorganism bacterium agent for resisting bacterial wilt of eggplant
A technology of compound microbial inoculum and production process, applied in the directions of biocides, chemicals for biological control, biocides, etc., can solve the problems of inability to achieve long-term disease resistance, waste of traditional Chinese medicine resources, and insignificant effects. To achieve the effects of enhancing plant stress resistance, high survival rate, and simple and easy production process
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Embodiment 1
[0025] a) Preparation of Bacillus colloidal bacteria: the strain was purchased from Beina Bio-tech, strain number BNCC173119, strain culture medium 50kg, formula was peptone 0.5kg, beef extract 0.15kg, sodium chloride 0.25kg, pH7.5,121 Sterilize at ℃ for 30min, connect to purchase bacteria, culture at 150r / min in a shaker for 24h to obtain the culture liquid; prepare 1t amplification medium, the formula is 40kg beef extract, 9kg yeast extract powder, 3kg sodium chloride, pH7.5 , Transfer to the fermentation tank for high temperature sterilization, after sterilization, when the temperature is reduced to 36 ℃, insert 50kg of bacterial seed liquid, rotate at 120r / min for 26h, transfer out the fermentation broth 4000r / min and centrifuge for 30min to obtain Bacillus colloidalis Bacteria 128.7kg.
[0026] b) Preparation of Trichoderma harzianum: purchased from the strain, purchased from the Trichoderma Strain Collection and Management Center of Shanghai Jiaotong University, prepared th...
experiment example 1
[0033] Experimental example 1: Potted eggplant bacterial wilt prevention test
[0034] The composite microbial inoculum produced by the method of Example 1 was used for potted disease prevention test. The test steps are as follows:
[0035] The experimental eggplant variety "Zi Rong No. 2" was purchased locally. The bacterial wilt pathogen Ralstonia solanacearum was purchased from Beina biological number BNCC221120. The experimental soil was taken from Datian, Youyi Village, Laishan Town, Yantai City, which is prone to eggplant bacterial wilt. The soil, texture sandy soil, pH=5.3, organic matter 7.3g / kg, total nitrogen (N) 0.762g / kg, total phosphorus (P 2 O 5 )0.577g / kg, total potassium (K 2 O) 15.3g / kg. There are 3 treatments in the experiment, namely: T1: use Jinzhengda antimicrobial agent for root irrigation, the application rate is 3ml / m 2 ; T2: Use the fungus of the present invention for spreading, the application rate is 5g / m 2 ; T3: No inoculation, as a control. A three-sea...
experiment example 2
[0041] Experimental example 2: Field trial of eggplant bacterial wilt control
[0042] The composite microbial inoculum produced by the method of Example 1 was used for field trials. Field trials were conducted in Xiaohezi Village, Fushan District, Yantai City from April 2016 to September 2016. The test field was the incidence of bacterial wilt in recent years. For more serious plots, and after organic fertilizer application, the experimental eggplant variety is Fengyan 2. Two treatments were set up in the experiment, respectively: T1: Use the inocula of the present invention for spreading, the application rate is 5kg / 667m 2 ; T2: No inocula, as a control group. Each treatment is repeated 4 times, with a plot area of 100m 2 .
[0043] Observe the disease on 30d, 60d, 90d, 120d after planting, disease grade: 0 is asymptomatic on the leaf surface; 1 is wilted on the leaf surface below 1 / 4 of the plant; 2 is 1 / 4 to 1 / 2 leaf The surface is wilting; the level 3 is that the leaf surf...
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