Application of fucoidan for enhancing storage stability of biological peptides

A technology of storage stability and fucoidan, which is applied in the field of biochemistry, can solve the problems of high cost and achieve the effects of preventing degradation, reducing activity and inhibiting growth

Inactive Publication Date: 2017-12-15
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Bioactive peptides, such as antimicrobial peptides, digestive enzymes, a

Method used

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  • Application of fucoidan for enhancing storage stability of biological peptides
  • Application of fucoidan for enhancing storage stability of biological peptides
  • Application of fucoidan for enhancing storage stability of biological peptides

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Experimental program
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Embodiment Construction

[0018] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. For the experimental methods that do not indicate specific conditions in the following steps, the conventional conditions are usually followed.

[0019] 1. Fucose and EGF

[0020] Both fucose and EGF are commercially available raw materials.

[0021] The fucose-free control EGF sample (0.05g EGF) and the fucose-containing experimental sample (0.05g EGF mixed with 0.15g fucose) were packed with weighing paper, then sealed in a plastic bag at Store at room temperature or 28°C for 12 months.

[0022] 2. Cell Proliferation Assay

[0023] To test the ability of fucose to maintain the bioactivity of stored EGF, cell proliferation studies were performed monthly. The stored EGF + / - fucose samples were taken out, resuspended in LB medium, filter sterilized, and then added to human embryonic kidney 293 (HEK293) cell culture. Cells were starv...

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Abstract

The invention provides an application of fucoidan for enhancing storage stability of biological peptides. According to the application, fucose is used as a bioactive polypeptide storage additive, so that the activity of the biological peptides can be kept for a relatively long time, structural degradation of the biological peptides can be prevented, and additional nutrient substances can be supplied for the biological peptides; and furthermore, the growth of airborne pollution bacteria in a storage bag can be inhibited, and the activity of protease in the storage bag can be reduced.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to the use of fucoidan for enhancing the storage stability of biological peptides. Background technique [0002] Fucoidan is a natural polymer present in brown algae, mainly composed of fucose monomers, which has antibacterial, antioxidant, anti-inflammatory and antitumor effects. Studies have shown that the scavenging ability of fucoidan on superoxide anions and hydroxyl radicals is much stronger than that of synthetic antioxidants. Fucose is a prebiotic for the human body that promotes gut commensal bacteria and prevents colonization of the gut by other microorganisms. For example, fucosylated oligosaccharides from milk promote the growth of commensal Bifidobacteria but inhibit the growth of Escherichia coli in the mammalian gastrointestinal tract. [0003] Bioactive peptides, such as antimicrobial peptides, digestive enzymes, and cell growth factors, have various health-...

Claims

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Application Information

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IPC IPC(8): A23L3/3562A23L33/125A23L33/18
CPCA23L3/3562A23L33/125A23L33/18A23V2002/00A23V2250/50A23V2250/55A23V2200/32
Inventor 李巨浪孙晓凤艾米丽·希尔
Owner FOSHAN UNIVERSITY
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