Collagen fiber protein composite bleeding stopping patch and preparation method thereof
A collagen fiber and collagen technology, which is applied in the fields of biomedical engineering and material science, can solve the problems of allergic reactions, slow hemostasis, and increased wound bleeding.
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Embodiment 1
[0015] a. Extraction of fibrinogen
[0016]After adding 95% cold ethanol at -20°C (10% ethanol by volume) to pig plasma, a precipitate was produced, which was collected by centrifugation, and the supernatant was used to extract prothrombin. The precipitate was washed with 0.3% NaCl aqueous solution at 0°C. Primary dissolution: add 1.3% NaCl aqueous solution of primary dissolving agent to the washed precipitate and heat to 36°C while stirring to fully dissolve, then centrifuge to remove insoluble matter and collect the supernatant; secondary precipitation: add to the separated supernatant 95% cold ethanol at -20°C (10% ethanol by volume) was used to precipitate fibrinogen, and the supernatant was removed by centrifugation to collect the precipitate. Second washing: wash with 0.3% Nacl aqueous solution at 0°C, and centrifuge to collect the precipitate, which is pure fibrinogen. If the precipitate after the second washing can not be fully dissolved by adding a 1.3% NaCl aqueous...
Embodiment 2
[0022] a. Extraction of fibrinogen
[0023] After adding 95% cold ethanol at -21° C. (11% ethanol by volume) to bovine plasma, a precipitate was produced, which was collected by centrifugation, and the supernatant was used to extract prothrombin. The precipitate was washed with 0.4% NaCl aqueous solution at 1°C. Primary dissolution: add 1.4% NaCl aqueous solution of primary dissolving agent to the washed precipitate and heat to 37°C while stirring to fully dissolve, then centrifuge to remove insoluble matter and collect the supernatant; secondary precipitation: add to the separated supernatant 95% cold ethanol (11% ethanol by volume ratio) was used to precipitate fibrinogen at -21°C, and the supernatant was removed by centrifugation to collect the precipitate. Second washing: wash with 0.4% Nacl aqueous solution at 1°C, and centrifuge to collect the precipitate, which is pure fibrinogen. If the precipitate after the second washing can not be fully dissolved by adding a 1.4% ...
Embodiment 3
[0029] a. Extraction of fibrinogen
[0030] After adding 95% cold ethanol at -23°C (13% ethanol by volume) to sheep plasma, a precipitate was produced, which was collected by centrifugation, and the supernatant was used to extract prothrombin. The precipitate was washed with 0.4% NaCl aqueous solution at 2°C. Primary dissolution: add 1.5% NaCl aqueous solution of primary dissolution agent to the washed precipitate and heat to 38°C while stirring to fully dissolve, then centrifuge to remove insoluble matter and collect the supernatant; secondary precipitation: add to the separated supernatant 95% cold ethanol (13% ethanol by volume ratio) was used to precipitate fibrinogen at -23°C, and the supernatant was removed by centrifugation to collect the precipitate. Second washing: wash with 0.4% Nacl aqueous solution at 2°C, and centrifuge to collect the precipitate, which is pure fibrinogen. If the precipitate after the second washing can not be fully dissolved by adding a 1.5% Na...
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