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Biological preservative based on alginate-derived oligosaccharides and application thereof in preservation of prawns

A biological preservative, the technology of brown algae oligosaccharide, applied in the directions of biochemical equipment and methods, preservation of meat/fish with chemicals, lyase enzymes, etc., can solve the problems of reducing the shelf life of prawns, decreasing the freshness, and economic losses of fishermen, etc. Achieving good preservation, pH improvement, and no potential dangers

Active Publication Date: 2017-12-22
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, shrimps generally die soon after being caught, and because the moisture content of shrimp meat is high and the tissue protein is high, it is very suitable for the growth and reproduction of microorganisms, resulting in a rapid decline in its freshness and easy corruption, which reduces the shelf life of prawns. It is used for long-distance transportation, resulting in a great waste of resources and a great economic loss to fishermen.
[0003] The traditional fresh-keeping methods are mainly low-temperature preservation and chemical preservative preservation, but physical preservation has the disadvantages of high requirements on external conditions, complicated procedures, unfavorable operation, and high cost of use, which is not conducive to fishermen's post-fishing operations; chemical preservative preservation is the best Sulfite is commonly used, but when fishermen use sodium metabisulfite to preserve fresh shrimp, most of them use the direct dusting method. Although this method is easy to operate, it is difficult to spread evenly, so it is often used in excess, and excessive residual sulfite will cause cause certain harm to the human body

Method used

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  • Biological preservative based on alginate-derived oligosaccharides and application thereof in preservation of prawns
  • Biological preservative based on alginate-derived oligosaccharides and application thereof in preservation of prawns
  • Biological preservative based on alginate-derived oligosaccharides and application thereof in preservation of prawns

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Experimental program
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Effect test

Embodiment 1

[0022] 1) Inoculate the strain producing alginate lyase in the liquid medium with an inoculum of 3% (v / v), culture with shaking for 16 hours; then transfer to the fermentation medium with an inoculum of 3% (v / v) culture at 30°C and 180r / min with shaking for 36h; the obtained bacterial liquid was centrifuged at 10000r / min at 4°C, and the supernatant was added with (NH 4 ) 2 SO 4 Make the saturation reach 40%, let it fully precipitate overnight at 4°C, centrifuge at 11000r / min at 4°C the next day, take the supernatant and add (NH 4 ) 2 SO 4 Make the saturation reach 60%, let it fully precipitate overnight at 4°C, then centrifuge at 11800r / min at 4°C the next day, dialyze the obtained precipitate with phosphate buffer solution with pH=7.2 for 12-24h, and obtain the material in the dialysis bag. is the crude enzyme solution;

[0023] 2) Mix sodium alginate with crude enzyme solution and distilled water at a mass ratio of 1:2:50, degrade in a constant temperature water bath at...

Embodiment 2

[0029] The prawns were kept alive and transported back; abnormal individuals such as dead, incomplete limbs, and discoloration were removed; the selected prawns were rinsed with sterile water, put into crushed ice to make the prawns die suddenly, and then randomly divided into three groups. The blank control group used distilled water as a preservative, the positive control group used sodium metabisulfite as a preservative, and the experimental group used the biological preservative of Example 1 containing fucoidan oligosaccharides. According to the mass volume ratio of prawns and preservatives 1g:1L, soak the prawns in preservatives for 30min, drain them, pack them in fresh-keeping bags, and store them at 4°C. Observe and measure every 48h.

[0030] (1) Volatile basic nitrogen (TVB-N)

[0031] Accurately weigh 2 g of each prawn sample, and measure the production of volatile basic nitrogen in the three groups of prawns at different storage times by the semi-micro nitrogen det...

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Abstract

The invention discloses a biological preservative based on alginate-derived oligosaccharides and application thereof in preservation of prawns, and belongs to the technical field of preservation and processing of aquatic products. The biological preservative based on the alginate-derived oligosaccharides is prepared from sodium alginate as a substrate by an enzymolysis method. The biological preservative is safe, has no potential danger, is convenient to use, has good preservation effect on the prawns, can effectively delay corruption of prawns, and has good application value.

Description

technical field [0001] The invention belongs to the technical field of preservation and processing of aquatic products, and in particular relates to a biological preservative based on fucoidan oligosaccharide and its application in preservation of prawns. Background technique [0002] Among seafood products, prawns are favored by consumers for their delicious meat, high protein content, low fat content and high nutritional value. In recent years, many countries have carried out artificial breeding of prawns, which has led to a substantial increase in the global prawn production. Now my country has become a big country in prawn farming. Fujian Province is adjacent to the sea and is an important province for the export of prawn farming. However, shrimps generally die soon after being caught, and because the moisture content of shrimp meat is high and the tissue protein is high, it is very suitable for the growth and reproduction of microorganisms, resulting in a rapid decline ...

Claims

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Application Information

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IPC IPC(8): A23B4/20C12N9/88
CPCA23B4/20C12N9/88C12Y402/02003
Inventor 严芬李晓月翁祖铨吴晨烁
Owner FUZHOU UNIVERSITY
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