Pistacia weinmannifolia extract, preparation method and applications thereof, and skin external application preparation containing Pistacia weinmannifolia extract

A kind of skin external preparation, the technology of fragrant wood, which is applied in the field of fragrant wood extract and its preparation, and can solve the problems such as no report of fragrant wood found.

Inactive Publication Date: 2017-12-22
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the research progress in recent years, no re

Method used

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  • Pistacia weinmannifolia extract, preparation method and applications thereof, and skin external application preparation containing Pistacia weinmannifolia extract
  • Pistacia weinmannifolia extract, preparation method and applications thereof, and skin external application preparation containing Pistacia weinmannifolia extract
  • Pistacia weinmannifolia extract, preparation method and applications thereof, and skin external application preparation containing Pistacia weinmannifolia extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Fragrance wood extract

[0046] (1) Fragrant wood petroleum ether part

[0047] Weigh 100 g of the stems and leaves of A. fragrans, pulverize, and extract 2 times by cold soaking with 800 ml of petroleum ether for 24 hours each time.

[0048] (2) Fragrant wood crude extract

[0049] Weigh 50g of petroleum ether cold-soaked and extracted residue, extract 2 times with 400ml 85v / v% ethanol aqueous solution at 500W ultrasonic for 2 hours each time, filter, combine the filtrates, and evaporate the filtrate to dryness to obtain 8.5g of the crude extract of A.

[0050] (3) 50% and 90% of Fragrant Wood

[0051] Weigh 7g of the crude extract of Fragrantia chinensis, dissolve it with 35ml of 30v / v% ethanol aqueous solution, pass through the macroporous adsorption resin D101, first eluate with 1 column volume of deionized water, and discard the washing solution. Then use 5 times the column volume of 50% v / v% ethanol solution and 90% v / v% ethanol solutio...

Embodiment 2

[0052] Example 2 Antioxidant activity of extracts

[0053] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical. The DPPH method was proposed in 1958 and is widely used to quantitatively measure the anti-aging ability of biological samples, classified substances and foods. This method is based on the fact that the DPPH free radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to its single electron pairing, and the number of electrons is quantitatively related to its fading degree and its acceptance. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect free radical scavenging. , so as to evaluate the anti-aging ability of the samples.

[0054] Take the crude extract of Fragrantia orientalis prepared in Example 1 and the eluted parts of each ethanol solution to prepare 0.1 mg / m...

Embodiment 3

[0063] Example 3 Study on the elastase inhibitory effect of the extracts

[0064] Elastase is a proteolytic enzyme characterized by decomposing insoluble proteins, which can cause excessive degradation of elastin in the dermis of the skin that supports the skin structure, resulting in aging symptoms such as wrinkles, sagging and inelastic skin. Protease inhibitors can effectively inhibit the activity of elastase, thereby enhancing skin elasticity, reducing wrinkles and pigmentation, and delaying skin aging.

[0065] The Fragrance wood extract prepared in Example 1 was prepared to 6 mg / mL, and the inhibitory effect of elastase was determined by the following method. Set up sample tube and sample solvent control tube, blank control and blank solvent control. Add 10 μL of sample solutions of different concentrations to each of the sample tube and the sample solvent control tube, and replace the blank control and blank solvent control tubes with 10 μL of 0.1M Tris-HCl buffer solu...

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Abstract

The invention discloses a Pistacia weinmannifolia extract, a preparation method and applications thereof, and a skin external application preparation containing the Pistacia weinmannifolia extract. According to the present invention, the Pistacia weinmannifolia extract extract can significantly clear free radicals and inhibit elastase and tyrosinase, and can be used in the fields of cosmetics, health food and medicine, particularly in cosmetics.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a fragrant wood extract and a preparation method and application thereof. Background technique [0002] In 1956, Harman proposed the theory of free radical aging [Harman, D. Aging: A theory based on freeradical and radiation chemistry. J. Gerontol. 1956, 11:289-300.], thinking that excessive free radicals cause biological aging important reason. According to this theory, excess active oxygen free radicals in the body react with unsaturated fatty acids to produce substances such as malondialdehyde, and malondialdehyde will react with proteins on the cell membrane to produce brown pigments, which will precipitate on the skin and become various stains. Excessive free radicals can also cross-link and degenerate collagen fibers and elastic fibers in the skin, become brittle, and lose elasticity. When the skin lacks moisture, it is easy to break the elastic fibers, resulting in ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/97A61K2800/805A61K2800/82A61Q19/00A61Q19/02A61Q19/08
Inventor 章漳李慧张佳伟宋肖洁席淑云陈明华
Owner JALA GROUP CORPORATION
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