Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving senescence state of mesenchymal stem cells of aged rats

A bone marrow mesenchymal and stem cell technology, applied in the direction of bone/connective tissue cells, cells modified by introducing foreign genetic material, animal cells, etc. Improves effects of age-related aging

Inactive Publication Date: 2017-12-22
ZHEJIANG UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stem cells are cells with vigorous division and proliferation. DNA damage and telomere shortening are significantly increased during the process of DNA synthesis and metabolism in cells. Although stem cells express telomerase activity, this is not enough to compensate for the telomere shortening caused by cell division.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving senescence state of mesenchymal stem cells of aged rats
  • Method for improving senescence state of mesenchymal stem cells of aged rats

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Obtain bone marrow mesenchymal stem cells from aged rats.

[0020] The 18-month-old SD rats were killed by neck dislocation, the fur was disinfected with 75% alcohol, the bilateral femur and tibia were taken out, the soft tissue and bone debris on the bone surface were removed, and put into a centrifuge tube filled with low-sugar DMEM medium. The following operations were performed on a clean bench except for centrifugation. Pour the bone tissue into a sterile glass Petri dish. Use ophthalmic scissors to cut a little at both ends of the femur and tibia to expose the marrow cavity. Use a 5ml syringe to extract the cell culture solution (low-sugar DMEM containing 10% fetal bovine serum, 100U / ml penicillin and 100U / ml streptomycin) to repeatedly rinse the bone marrow cavity; collect the rinsing solution in a 50ml sterile centrifuge tube. Centrifuge at 1000 rpm for 8 minutes at 4°C. Discard the supernatant and resuspend the cells in culture medium. The cells obtained...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a gene engineering method for improving age-related senescence of mesenchymal stem cells of aged rats. The method comprises the following steps: putting passage-stable mesenchymal stem cells obtained from marrow of the aged rats in a conventional culture medium provided with TPP1 overexpression slow virus particles (MOI is 100), infecting for 24 hours, and then replacing the culture medium into the conventional culture medium, adding puromycin in concentration of 5 microgrammes per microliter after 3 days, screening for 7 days, and applying the screened mesenchymal stem cells to cell transplantation therapy. The method has the beneficial effects that through overexpression of TPP1 in the mesenchymal stem cells of the aged rats, the age-related senescence of the mesenchymal stem cells of the aged rats can be obviously improved, so that the purpose of improving the effect of treating ischemic myocardial diseases can be achieved.

Description

(1) Technical field [0001] The invention relates to a method for improving the age-related aging state of aged rat bone marrow mesenchymal stem cells. (2) Background technology [0002] Ischemic cardiomyopathy is one of the killers that seriously threaten human health, with a high incidence of sudden death. Most patients inevitably develop heart failure despite aggressive treatment. Although traditional treatment methods have greatly improved the survival rate and prognosis of patients, they cannot save the dying myocardium and fail to significantly improve the cardiac remodeling of patients after myocardial infarction. Bone marrow mesenchymal stem cells have attracted extensive attention and research in the field of regenerative medicine in recent years because of their self-renewal and multilineage differentiation capabilities. A large number of clinical studies have confirmed its safety and effectiveness in the treatment of cardiovascular diseases. However, the low surv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/10
CPCC07K14/4702C12N5/0663
Inventor 刘先宝王建安曾智茹刘丰
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com