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Vaccines against avian genetype VII Newcastle disease and preparation method thereof

A genotype and vaccine technology, applied in antiviral agents, viral antigen components, etc., can solve problems such as unsatisfactory effects

Inactive Publication Date: 2017-12-29
GUONIAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the application of this type of vaccine can protect chickens to some extent from the challenge of NDV virus strains, the effect is still unsatisfactory

Method used

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  • Vaccines against avian genetype VII Newcastle disease and preparation method thereof
  • Vaccines against avian genetype VII Newcastle disease and preparation method thereof
  • Vaccines against avian genetype VII Newcastle disease and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1～ preparation example 3

[0061] "Table 1"

[0062]

[0063]

[0064] "Preparation of Deactivated Vaccine"

preparation example 4～ preparation example 6

[0066] First, one of the VII genotype Newcastle disease virus strains (deposited in China Center for Type Culture Collection, deposit numbers CCTCC NO:V201548, CCTCC NO:V201555, CCTCC NO:V201556, The storage date is December 23, 2015) as the source of infection, and then inoculated in the allantoic cavity of 9-day-old specific-pathogen-free (SPF) chicken embryos, or BHK-21 cells as shown in Table 1 (C-13) (ATCC CCL-10) host, and then incubator (Alex Alex electrical processing plant, AI-528) or incubator (Thermo Scientific Forma, Cat.No.311) respectively at 37 ℃ ), and then after 48 hours of culture, the culture fluid was extracted from the above-mentioned chicken embryo allantoic cavity and BHK-21 cells and centrifuged to obtain the VII genotype Newcastle disease antigen solution M4-M6.

[0067] Then, the above-mentioned Genotype VII Newcastle Disease Antigen Solution M4-M6 and Formalin (HT501128-4L, manufactured by Sigma Co.) 20% solution were prepared at a volume ratio of An...

Embodiment 1～ Embodiment 3

[0073] The above-mentioned live virus vaccines VL1~VL3 were administered to immunized 2-3 week-old chickens by nasal and subcutaneous injection respectively, and 6 chickens were administered in each embodiment, and the vaccine dose administered to each chicken was 0.2 ml, blood was collected 3 weeks later to separate serum S1-S24.

[0074] Next, carry out the hemagglutination (HA) test, that is, add 50 μl of HI buffer (3.25g KH 2 P0 4 , 10.8g Na 2 HP0 4 , 170g NaCl, and purified water after three deionized reactions to 2,000mL), and then add 50μl of Genotype VII Newcastle Disease Antigen Solution to the first column.

[0075] Next, after mixing thoroughly with a micropipette, take 50 μl of the Newcastle disease antigen solution of genotype VII in the first row and add it to the second row, and then take another 50 μl and add it to the third row, so that the sequence is diluted to the tenth row, and the antigen presents 2 times to 1024-fold dilution. In row 11 as the contr...

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Abstract

The invention provides vaccines against the avian genetype VII Newcastle disease and a preparation method thereof. The live virus vaccines against the avian genetype VII Newcastle disease at least comprise antigen solutions and a diluent, wherein the live virus antigen solutions are prepared in virtue of SPF chick embryo allantoides and / or BHK-21 cells infected by virus strains from chickens with the Newcastle disease, and the virus strains are preserved in China Center for Type Culture Collection on December 23rd, 2015, with accession numbers of CCTCC No. V201548, CCTCC No. V201555, and CCTCC No. V201556, Moreover, the inactivated vaccines against the avian genetype VII Newcastle disease at least comprise inactivated antigen solutions and oil adjuvant, wherein the inactivated antigen solutions are prepared in virtue of SPF chick embryo allantoides and / or BHK-21 cells infected by virus strains from chickens with the Newcastle disease.

Description

technical field [0001] The present invention relates to a vaccine suitable for poultry and its preparation method, in particular to a poultry genotype VII Newcastle Disease (Newcastle Disease, ND) vaccine and its preparation method. Background technique [0002] According to the research results of Taiwan (Ke et al., 2008) and South Korea (Jeon et al., 2008), it is pointed out that the main cause of vaccine immunity failure and ND outbreak in Asia is the antigenic variation of genotype VII (Jeon et al. al., 2008; Boven et. al., 2008). Vaccines generally play an integral and important role in the various means used to control pandemic diseases including, for example, poultry Newcastle disease. In particular, in the case where a specific strain of the epidemic-like virus causes a large number of infections and a major epidemic breaks out in a short period of time, it is generally impossible to control the environment by physical means such as sanitation, or to use antibiotics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61P31/14
Inventor 林建宏
Owner GUONIAN IND CO LTD
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