Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ectopic expression vector and its application in improving plant vegetative tissue oil content

A vegetative tissue and carrier technology, applied in the field of genetic engineering, can solve the problems of tissue distortion, somatic embryogenesis of seedlings, and affect the normal growth of transgenic plants, etc., and achieve the effect of increasing oil content

Active Publication Date: 2020-12-22
ZHEJIANG FORESTRY UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequently, Mendoza et al. overexpressed the transcription factor LEAFY COTYLEDON2 (LEC2) involved in the regulation of seed maturation and oil accumulation in Arabidopsis, and found that seed-specific mRNA was accumulated in vegetative tissues, and the storage TAG content increased, but At the same time, somatic embryogenesis occurred in the seedlings, and the tissues were distorted and deformed, which affected the normal growth of transgenic plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ectopic expression vector and its application in improving plant vegetative tissue oil content
  • Ectopic expression vector and its application in improving plant vegetative tissue oil content
  • Ectopic expression vector and its application in improving plant vegetative tissue oil content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0058] 1. Construction of transcription unit and screening of target gene

[0059] In order to construct different plant transcription units, the applicant selected 35S and BCH1, which can be constitutively expressed in vegetative organs, as promoters. In addition, the target genes involved in lipid synthesis were also selected: (i) GAT1, a gene encoding 3-phosphoglycerol acyltransferase in yeast; (ii) a gene DGAT1 encoding diacylglycerol acyltransferase, the former and the latter were involved in lipid synthesis, respectively. First and third acylation reactions of synthesis; (iii) oleosin 2 (OLEO2) encodes an oil body chimeric protein that maintains the integrity and stability of oil droplets; (iv) converts vegetative cells into embryogenic cells The regulatory factor LEC2, whose ectopic expression in leaves can increase the synthesis and accumulation of leaf oil.

[0060] Among the above four target genes, GAT1 is derived from Saccharomyces cerevisiae, DGAT1, OLEO2 and LEC...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to different gene combinations realizing ectopic expression and an application of the different gene combinations in increase of oil content in plant nutrient tissue, and belongs to the field of genetic engineering. Four genes, namely, LEC2, DGAT1, OLEO2 and yeast GAT1, involved in the different oil synthesis and accumulation processes respectively are selected to be linked with promoters capable of driving the genes to express in the plant nutrient tissue, and different expression vectors consisting of a single gene or multiple genes are constructed and transferred in the plant tissue, so that the target genes express ectopically or heterogeneously in the plant nutrient tissue; finally, transgenic plants with significantly increased oil content in the nutrient tissues are obtained by screening and are applied to production and application of various substances associated with vegetable oil, for example, increase of nutrients of animal feed and promotion production of biofuel.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to different gene combinations of ectopic expression and its application in increasing the oil content of plant vegetative tissues. Background technique [0002] Vegetable oils are versatile and are not only a major source of cooking oil, but are also used in animal feed, soaps, surfactants, cosmetics, paints, lubricants, and in the production of biodiesel. According to reports, the consumption of edible oil will double before 2030; the chemical industry also hopes that vegetable oil can replace 40% of crude oil in 20 years, so the demand for vegetable oil is increasing sharply. However, due to population growth and reduction of arable land, China will face a severe shortage of edible oil and chemical crude oil. It is hoped that by improving and changing the agronomic and quality traits of oil plants (including seed yield, oil content and fatty acid compositi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/66A01H5/00A01H6/82A01H6/54A01H6/20A01H6/14A01H6/46
CPCC12N15/66C12N15/8247
Inventor 牛蕾蕾雷洁郑志富甘毅陈亚东薛金嫚刘宏波
Owner ZHEJIANG FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com