Coconut chaff extract and application thereof in synergistic oxidation resistance

A technology of extract and coconut peat, applied in the field of coconut peat extract and its application in synergistic anti-oxidation, can solve the problems that the application has not been reported, and achieve the effect of enhancing the scavenging ability and enriching the use

Inactive Publication Date: 2018-01-09
深圳碳希生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coconut husk and coconut peat (that is, coconut fiber powder) are by-products of coconut deep processing. At present, coconu

Method used

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  • Coconut chaff extract and application thereof in synergistic oxidation resistance

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] (1) Take the dried coconut fiber (1.0kg) and pulverize to 100 mesh (to obtain coconut bran), add 5.0kg of absolute ethanol, reflux and extract for 5 hours, then filter while hot to remove the filter residue, and the filtrate is concentrated under reduced pressure to obtain leaching paste;

[0020] (2) The extract obtained in step (1) is adsorbed on a macroporous resin column and eluted with ethanol with a volume fraction of 60-70%. Collect 4-6 times the column volume of the eluent, concentrate under reduced pressure, and dry under vacuum Obtained coconut bran extract (18.9g, hereinafter referred to as product A).

Example Embodiment

[0021] Example 2

[0022] (1) Take the dried coconut fiber (1.0kg) and crush it to 20 mesh (to obtain coconut bran), add 10.0kg of absolute ethanol, reflux and extract for 8 hours, filter while hot, remove the filter residue, and concentrate the filtrate under reduced pressure to obtain a leaching paste;

[0023] (2) The extract obtained in step (1) is adsorbed on a macroporous resin column and eluted with ethanol with a volume fraction of 60-70%. Collect 4-6 times the column volume of the eluent, concentrate under reduced pressure, and dry in vacuum Obtained coconut bran extract (20.2g, hereinafter referred to as product B).

Example Embodiment

[0024] Example 3 Free radical scavenging ability of test sample

[0025] According to the method of the prior art, test rutin, product A, product B, product A + rutin (the mass ratio of the two is 1:1), and product B + rutin (the mass ratio of the two is 1: 1) are free to hydroxyl And DPPH free radical scavenging ability, calculate the half inhibitory concentration (IC 50 Value), the following test results of the present invention were tested according to the method described in Chinese patent application number: 201310089721.X, see Table 1.

[0026] Table 1

[0027]

[0028] "+++" means the compound concentration is 0.1-1.0μg / mL, "++" means the compound concentration is 10-100μg / mL, "-" means the compound concentration is greater than 1×10 5 μg / mL.

[0029] The above experimental results show that products A and B have almost no free radical scavenging ability, but when they are mixed with rutin at a mass ratio of 1:1, it can significantly enhance the scavenging ability of rutin on f...

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Abstract

The invention relates to coconut chaff extract and application thereof in preparing an antioxidant. The coconut chaff extract is prepared through a following method comprising the following steps: (1)taking coconut chaff, adding 5 to 10 mass times of absolute ethanol, immediately filtering after carrying out reflux extraction for 5 to 8 hours, removing filter residue, and carrying out vacuum concentration on filtrate, thus obtaining an extract; (2) carrying out macroporous resin column adsorption on the extract obtained in the step (1), eluting by using ethanol of which the volume percentageis 60 to 70 percent, collecting 4 to 6 column volume times of eluate, carrying out vacuum concentration, and carrying out vacuum drying, thus obtaining the coconut chaff extract. The coconut chaff extract disclosed by the invention is capable of enhancing the scavenging capacity of rutin on hydroxyl free radicals and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals, can be developed and used forpreparing the antioxidant, and can be used in the fields of medicine, food, cosmetics and the like.

Description

technical field [0001] The invention belongs to the field of whitening and sun protection, and in particular relates to a coconut bran extract and its application in synergistic anti-oxidation. Background technique [0002] Coconut (Cocos nucifera L.) is a monocotyledonous plant of the genus Cocos in the palm family, and is a tropical economic crop with great development value. Coconut meat is edible and can also be used for oil extraction, and coconut water (endosperm) can be used for beverages and the like. Coconut husk and coconut peat (i.e. coconut fiber powder) are by-products of coconut deep processing. At present, coconut peat is mainly used in agriculture and forestry as a cultivation substrate, and there are no reports on other applications. The present invention uses an organic solvent to extract coconut bran, and finds that the extract has the ability to enhance the ability of rutin to clear free radicals. Therefore, the coconut bran extract can be developed to p...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61Q19/02A61Q19/08A61Q17/04A61K36/889A61P39/06A23L33/105A61K31/7048
Inventor 郑超秦伟张宏李礼如李音
Owner 深圳碳希生物科技有限公司
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