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Method for extracting acaciacin-6-c-β-d-glucoside from sorrel

A technology of -6-C-, glucoside, which is applied in the field of extracting high-purity locustin-6-C-β-D-glucoside, which can solve the problems of low purity of active ingredients, restrictions on the development of Oxalis herb and its preparations Questions about quality control, reference substances, etc.

Active Publication Date: 2018-10-09
贵州维康子帆药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no reference substance of sorrel monomer. The main reason is: when the existing method extracts and separates sorrel, the purity of the active ingredients obtained is low, and it cannot be used as a reference substance of sorrel monomer.
Limits the development and quality control of wood sorrel and its preparations

Method used

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  • Method for extracting acaciacin-6-c-β-d-glucoside from sorrel
  • Method for extracting acaciacin-6-c-β-d-glucoside from sorrel
  • Method for extracting acaciacin-6-c-β-d-glucoside from sorrel

Examples

Experimental program
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Effect test

Embodiment 1

[0054] A method for extracting acaciacin-6-C-β-D-glucoside from wood sorrel: adding 6 times the amount of water to decocting wood sorrel 3 times, each time for 60 minutes, combining the decoctions, filtering, and concentrating the filtrate to 60 The extract with a relative density of 1.31-1.35 at -65°C is dried and pulverized into powder. The powder is dispersed with 1.5 times the amount of water, put into a separatory funnel, and extracted several times with ethyl acetate to obtain ethyl acetate extract layer extract, the ethyl acetate layer extract is dissolved in chloroform, add 1 times the amount of silica gel and mix well, and evaporate the solvent; another 30 times the amount of the extract is loaded into the normal pressure chromatographic column by dry method; Silica gel column chromatography is used for separation, the extract is loaded into an atmospheric silica gel column, and covered with a layer of 0.5cm thick silica gel; chloroform-methanol=9:1-4:1 system is divid...

Embodiment 2

[0056] A method for extracting acacinin-6-C-β-D-glucoside from wood sorrel: adding 7 times the amount of water to decocting wood sorrel 3 times, each time for 70 minutes, combining the decoctions, filtering, and concentrating the filtrate to 60 The extract with a relative density of 1.31-1.35 at -65°C is dried and pulverized into powder, the powder is dispersed with 2 times the amount of water, put into a separatory funnel, and extracted several times with ethyl acetate to obtain ethyl acetate extract Layer extract, ethyl acetate layer extract is dissolved in chloroform, add 1.5 times the amount of silica gel and mix well, evaporate the solvent; take another 32 times the amount of the extract of silica gel dry method into the normal pressure chromatographic column; use normal pressure Silica gel column chromatography is used for separation, the extract is loaded into an atmospheric pressure silica gel column, and covered with a layer of silica gel; chloroform-methanol=9:1-4:1 s...

Embodiment 3

[0058] A method for extracting acacinin-6-C-β-D-glucoside from sorrel: adding 5 times the amount of water to decocting sorrel twice, each time for 50 minutes, combining the decoctions, filtering, and concentrating the filtrate to 60 The extract with a relative density of 1.31-1.35 at -65°C is dried and crushed into a powder. The powder is dispersed with 1 times the amount of water, put into a separatory funnel, and extracted several times with ethyl acetate to obtain ethyl acetate. Layer extract, the ethyl acetate layer extract is dissolved in chloroform, add 0.5 times the amount of silica gel and mix well, and evaporate the solvent; take another 28 times the amount of the extract and put it into the normal pressure chromatographic column by dry method; Silica gel column chromatography for separation, put the extract into a normal pressure silica gel column, and cover with a layer of 2cm thick silica gel; chloroform-methanol=9:1-4:1 system is divided into 6 gradients for elutio...

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Abstract

The invention provides a method for extracting acacetin-6-C-beta-D-glucopyranoside from Oxalis corniculata L.. The method comprises the following steps: extracting the Oxalis corniculata L. with waterto obtain Oxalis corniculata L. water extract, drying and crushing the Oxalis corniculata L. water extract to obtain powder, carrying out reduced pressure concentration through a rotary evaporator after the extraction to obtain ethyl acetate extract, and separating the ethyl acetate extract to obtain the acacetin-6-C-beta-D-glucopyranoside. When an effective component is separated from the Oxaliscorniculata L. extract through the method, the obtained effective component is highly pure, especially the purity of the acacetin-6-C-beta-D-glucopyranoside is 99% or more, the acacetin-6-C-beta-D-glucopyranoside can be used as an Oxalis corniculata L. monomer reference substance, and the development and the quality control of an Oxalis corniculata L. medicinal material and its preparation are facilitated.

Description

technical field [0001] The invention relates to a method for extracting effective components in sorrel, in particular to a method for extracting high-purity acaciacin-6-C-β-D-glucoside from sorrel. Background technique [0002] Sorrel (Oxalis corniculata L) is the dry whole grass of Oxalis corniculata L. Small perennial herb, the whole plant has a sour taste, so it is also called physalis grass, sour grass, dove grass, clover, etc. Produced in open space, roadsides and weeds, harvested in summer and autumn, distributed in Jiangxi and all over the country, and recorded in "Jiangxi Provincial Standards of Chinese Medicinal Materials" and "Shanghai Standards of Traditional Chinese Medicinal Materials". The whole plant contains locust-6-C-β-D-glucoside, citric acid, malic acid, tartaric acid, oxalate, etc. It is sour in nature and cool in nature, and has the functions of clearing heat and detoxifying, reducing swelling and promoting dampness. It can be used clinically to trea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 胡志林
Owner 贵州维康子帆药业股份有限公司
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