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Method for whitening agar under assistance of enzymatic method

An agar and enzymatic technology, applied in the field of enzymatic assisted whitening of agar, can solve problems such as environmental hazards, poor diffusion effect, and impact on human health, and achieve the effects of cost saving, reduction in usage, and sustainable development.

Inactive Publication Date: 2018-01-16
FUJIAN PROVINCE LVQI FOOD COLLOID +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium hypochlorite is a chemical reagent, used in large quantities, it will inevitably cause harm to the environment, and the chlorine gas released by sodium hypochlorite during the bleaching process is a poisonous gas with poor diffusion effect, which seriously affects human health

Method used

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  • Method for whitening agar under assistance of enzymatic method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Raw material processing: add 50 g of Gracilaria to rinse with water to remove sand, shells and other impurities;

[0028] Step 2. Alkali treatment: add 7 wt.% lye, the mass-to-volume ratio of material to liquid is 1:20, soak at a constant temperature at 90 °C for 3 h, filter, and rinse with water until neutral;

[0029] Step 3. Acidification treatment: Soak in water at a mass-to-liquid ratio of 1:18, then add 0.12 wt.% disodium edetate, 0.06% oxalic acid, and 0.06% sulfuric acid, treat for 40 minutes, filter, and rinse until neutral ;

[0030] Step 4. Bleaching treatment: Soak in water at a material-to-water mass volume ratio of 1:18, then add 0.25 wt. % sodium hypochlorite solution, process for 40 min, filter, and rinse until neutral;

[0031] Step 5: Boil glue and filter: soak in water at a mass-volume ratio of material to water of 1:12, boil under normal pressure until Gracilaria is completely melted, filter with plate and frame, remove algae residue, and ob...

Embodiment 2

[0035] Step 1. Alkali treatment: After drying and removing impurities, the Jiangli vegetable is added with a concentration of 7% lye, the ratio of solid to liquid is 1:18, soaked at a constant temperature of 85°C for 3 hours, filtered, and rinsed with water until neutral;

[0036] Step 2. Enzyme bleaching treatment: Add water at a material-to-liquid ratio of 1:18, then add 0.01% oxalic acid, adjust the pH value to 4.8-5.0 with acid, and add 0.1% laccase and 0.05% other compound enzymes at a constant temperature. Hydrolyze for 2 h, filter, and rinse until neutral; the complex enzymes are cellulase and laccase.

[0037] Step 3, remove metal ions: add water, the ratio of solid to liquid is the same as step 1, then add 0.012% EDTA disodium metal chelating agent, treat for 2 h, filter, and rinse until neutral;

[0038] Step 4, chemical bleaching treatment: add water with a material-to-liquid ratio of 1:18, then 0.1% sodium hypochlorite solution, process for 20 min, filter, and rins...

Embodiment 3

[0043] Step 1. Alkali treatment: After drying and removing impurity, Jiangli vegetable is added with 7% lye, the ratio of material to liquid is 1:18, soaked at a constant temperature of 85 °C for 3 hours, filtered, and rinsed with clean water until neutral;

[0044] Step 2. Enzyme bleaching treatment: add water at a material-to-liquid ratio of 1:18, then add 0.01% oxalic acid, adjust the pH value to 4.8-5.0 with acid, and add 0.1% laccase and 0.08% other compound enzymes at a constant temperature Hydrolyze for 2 h, filter, and rinse until neutral; the other complex enzyme is xylanase.

[0045] Step 3, remove metal ions: add water, the ratio of solid to liquid is the same as step 1, then add 0.012% EDTA disodium metal chelating agent, treat for 2 h, filter, and rinse until neutral;

[0046]Step 4, chemical bleaching treatment: add water with a material-to-liquid ratio of 1:18, then 0.1% sodium hypochlorite solution, process for 20 min, filter, and rinse until neutral;

[0047]...

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Abstract

The invention discloses a method for whitening agar under assistance of an enzymatic method. Gracilaria verrucosa is taken, put in an alkaline solution and treated at the constant temperature for 2-5h, the alkaline solution is removed, and the gracilaria verrucosa is blanched to be neutral; water and oxalic acid are added, the pH is adjusted with acid to range from 4.5 to 6.5, complex enzyme is added at the temperature of 45-60 DEG C, the gracilaria verrucosa is treated at the constant temperature for 1-3 h, an enzyme solution is removed, and the gracilaria verrucosa is rinsed to be neutral;a metal chelating agent is added, metal ions are removed, and the gracilaria verrucosa is filtered and rinsed to be neutral; a sodium hypochlorite solution is added, and the gracilaria verrucosa is treated for 20-60 min, filtered and rinsed to be neutral; water is added, and the gracilaria verrucosa is boiled to be completely molten and then filtered for residue removal; a gel solution is rapidlycooled, slit, subpackaged, subjected to oil-pressure dewatering, dried, crushed and sieved, and powder is collected. The method is applied to agar extraction through biological blanching under assistance of chemical blanching, usage of chemical agents is reduced, harm to body health is reduced, pollution to the environment is reduced, the production cost is saved, and the method has great significance for sustainable development of agar production enterprises.

Description

technical field [0001] The invention relates to the technical field of seaweed product extraction, in particular to a method for enzymatically assisted whitening agar. Background technique [0002] Agar is a natural polysaccharide extracted from red algae seaweed, and it is one of the most widely used seaweed gels in the world. Glue has excellent gelling, thickening and stabilizing properties, and is widely used in food, light industry, daily chemical industry, medicine and other fields. Agar is a marine natural polysaccharide substance, which contains more than ten kinds of beneficial mineral elements, polysaccharides and dietary fiber necessary for the human body, and has good health effects. In addition, agar can be further made into agarose, which is a good medium for immune diffusion and is widely used in the analysis and research of clinical diagnosis, biochemistry, microbiology, and immunology. [0003] At present, the raw materials for producing agar mainly include...

Claims

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Application Information

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IPC IPC(8): C08B37/12
Inventor 嵇海峰肖安风洪清林翁惠芬倪辉林坤城郭东旭肖琼蔡慧农姜泽东
Owner FUJIAN PROVINCE LVQI FOOD COLLOID
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