Spinal cord fusion agent, preparation method and use thereof

A fusion agent and spinal cord technology, which is applied in the field of spinal cord fusion products and its preparation, can solve problems such as inability to completely cure functional defects of patients, achieve the effects of inhibiting neuronal necrosis and local inflammation, reducing morbidity, and promoting nerve regeneration

Inactive Publication Date: 2018-01-19
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although clinical therapy can improve the quality of life of patients to a ce

Method used

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  • Spinal cord fusion agent, preparation method and use thereof
  • Spinal cord fusion agent, preparation method and use thereof
  • Spinal cord fusion agent, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 spinal cord fusion agent

[0027] 1. Materials and sources

[0028] The polyethylene glycol used in this example is a sterile liquid stock solution, purchased from sigma company; bone marrow mesenchymal stem cells are extracted from autologous bone marrow; Schwann cells are extracted from autologous sural nerve; GDNF is synthesized by the applicant of the present invention; Phenolic acid was purchased from Tianjin Tasly as an injection; nano-graphene microtubes were purchased from Mitsui Corporation of Japan as a powder.

[0029] 2. Method

[0030] (1) Weigh 0.1 g of bone marrow mesenchymal stem cells, 0.01 g of glial cell-derived neurotrophic factor (GDNF), 1 g of nano-graphene, 0.01 g of Schwann cells, 1 g of salvianolic acid, and polyethylene glycol 600 97.88g;

[0031] (2) Stir the weighed raw materials (sterile) evenly before use.

Embodiment 2

[0032] The preparation of embodiment 2 spinal cord fusion agent

[0033] 1. Materials and sources

[0034] With embodiment 1.

[0035] 2. Method

[0036] (1) Weigh 0.5 g of bone marrow mesenchymal stem cells, 0.01 g of glial cell-derived neurotrophic factor (GDNF), 0.05 g of nano-graphene, 0.1 g of Schwann cells, 3 g of salvianolic acid, polyethylene glycol 400 91.39g;

[0037] (2) Stir the weighed raw materials (sterile) evenly before use.

Embodiment 3

[0038] The preparation of embodiment 3 spinal cord fusion agent

[0039] 1. Materials and sources

[0040] With embodiment 1.

[0041] 2. Method

[0042] (1) Weigh 0.01 g of bone marrow mesenchymal stem cells, 0.05 g of glial cell-derived neurotrophic factor (GDNF), 1 g of nano-graphene, 1 g of Schwann cells, 1 g of salvianolic acid, and polyethylene glycol 800 96.94 g;

[0043] (2) Stir the weighed raw materials (sterile) evenly before use.

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Abstract

The invention discloses a spinal cord fusion agent and a preparation method and use thereof. The spinal cord fusion agent disclosed by the invention is prepared from polyethylene glycol, bone mesenchymal stem cells, Schwann cells, glial cell lined derived neurotrophic factor (GDNF), salvianolic acid and nano graphene. Studies show that the use of the spinal cord fusion agent of the invention enables rapid reconnection of divided white matter fiber tracts and gray matter staple fibers to reconstitute spinal integrity and electrical conduction and is capable of recovering motion and sensory functions below the lesion level in a short period of time, thereby effectively reducing the incidence of paraplegia and complications thereof, and no relevant products exist at home and abroad. The spinal cord fusion agent disclosed by the invention can have a wide application prospect clinically.

Description

technical field [0001] The invention relates to an intraoperative spinal cord fusion product and its preparation method and application, in particular to a product containing polyethylene glycol, bone marrow mesenchymal stem cells, glial cell-derived neurotrophic factor (GDNF), salvianolic acid, Spinal cord fusion product of nano-graphene, preparation method and application thereof. The invention belongs to the technical field of medical instruments. Background technique [0002] With the continuous advancement of science and technology, modern surgical techniques and methods are also advancing with each passing day, but spinal cord injury is still a major problem for neurosurgeons and orthopedic surgeons. Tens of thousands of people around the world suffer from spinal cord injuries every year, severe cases lead to paraplegia or even death, and the quality of life of patients is extremely low. [0003] Traditional medicine believes that the central nervous system cannot be...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K47/10A61K47/04A61P25/00A61K35/30A61K35/28A61K31/77A61K31/336
Inventor 任晓平刘泽汉任帅
Owner HARBIN MEDICAL UNIVERSITY
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