Gene expression profiles and uses thereof in breast cancer
A gene and breast cancer technology, applied in the field of predicting the local regional recurrence rate of breast cancer
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Embodiment 1
[0177] Gene microarray analysis to validate gene expression profiles associated with postoperative locoregional recurrence rates after mastectomy and breast-conserving surgery:
[0178] In an oncogene medicine study, patients with invasive breast cancer were eventually selected for the development of a new breast cancer classification. The study selected a total of 217 patients with invasive breast cancer who had undergone mastectomy or breast-conserving surgery between 2005 and 2012 and who had tissue samples available for DNA (or gene) microarrays. Of the 217 patients, 130 had undergone mastectomy and 87 had breast-conserving surgery. All 217 patients consented to have their primary tumor tissue subjected to DNA (or gene) microarray studies. Patients eligible for this study were not eligible for postmastectomy radiation therapy during at least a two-year follow-up period (n = 130). Patients who had undergone breast-conserving surgery (n=87) were analyzed separately to exam...
Embodiment 2
[0191] Gene expression map of locoregional recurrence rate validated by statistical analysis
[0192] The 34 genes in the new platform of the present invention are distributed in an 84-gene probe set, and 4 genes with unknown functions cannot be identified. By univariate analysis, 18 of the remaining 30 genes could classify mastectomy patients into a low-risk group and a high-risk group based on locoregional recurrence rate. Through multivariate analysis, it was found that patients could be classified into a low-risk group and a high-risk group. The 18 genes are used to classify breast-conserving surgery patients into a low-risk group and a high-risk group. Patients with an 18-gene score ≥ 31 were defined as high risk, while those with a score < 31 were defined as low risk.
[0193] The formula for calculating the score based on the 18 genes (18 gene detection combination or classification method)
[0194]Table 4 shows the univariate analysis and multivariate analysis using...
Embodiment 3
[0220] Cox proportional hazards model for mastectomy patients
[0221] According to current clinical practice, the method used to assess the need for adjuvant radiation therapy in N1 breast cancer patients will arrange adjuvant radiation therapy for 80% of them. However, radiotherapy reduces the rate of locoregional recurrence and improves overall survival in these patients.
[0222] Cox proportional hazards models have been widely used to describe survival and associated variables. Therefore, this study examined whether this (18-gene panel) classification was an independent prognostic factor. Traditional proportional hazards analysis has established clinical parameters related to proportional hazards and quantitative assessment, including the degree of lymph node metastasis and estrogen receptor status, which are related to locoregional recurrence rate. We applied the clinical parameters and the classification system of this classification to fully analyze the proportional ...
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