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Method for culturing microalgae by utilizing aquaculture wastewater

A technology for aquaculture wastewater and microalgae, applied in the direction of using microorganisms, methods based on microorganisms, chemical instruments and methods, etc., can solve the problems of no economic benefits, increase production costs, occupy indoor space, etc., and achieve a considerable environment Benefits and economic benefits, cost reduction and oil production costs, and the effect of resisting microbial attack

Inactive Publication Date: 2018-01-26
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of sterilizing and disinfecting wastewater from dairy farms is too high; on the other hand, the growth and reproduction of chlorella requires light, and indoor cultivation requires electricity and light sources, which takes up indoor space and increases the cost. Cost of production
It can be seen that using existing technologies to purify aquaculture wastewater and produce microalgae oil does not have economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The wastewater from the dairy farm was collected, precipitated overnight, filtered through gauze, and the supernatant was taken for later use. Its water quality indicators are as follows: pH value is 8.18, total nitrogen (TN) concentration is 295.73mg / L, total phosphorus (TP) concentration is 123.40mg / L, chemical oxygen demand (COD) is 2608.56mg / L, suspended solids ( SS) was 1.74.

[0025] Take chlorella algae and inoculate it into BG-11 medium at a concentration of 0.10g / L at an ambient temperature of 25°C and a light intensity of 800um m 2 ·S -1 , under the condition that the light time is 12 hours / day, acclimate and cultivate for 15 days. During the cultivation process, when the concentration of Chlorella reaches 1.5g / L, start to add unsterilized dairy farm wastewater into the medium , and every two days, 5% of the medium was replaced with an equal volume of aquaculture wastewater until the volume concentration of the aquaculture wastewater in the medium was 25%. ...

Embodiment 2

[0028] The wastewater from the dairy farm was collected, precipitated overnight, filtered through gauze, and the supernatant was taken for later use. Its water quality indicators are as follows: pH value is 8.18, total nitrogen (TN) concentration is 295.73mg / L, total phosphorus (TP) concentration is 123.40mg / L, chemical oxygen demand (COD) is 2608.56mg / L, suspended solids ( SS) was 1.74.

[0029] Take chlorella algae and inoculate it into BG-11 medium at a concentration of 0.08g / L, at an ambient temperature of 25°C and a light intensity of 800um m 2 ·S -1 , under the condition that the light time is 12 hours / day, acclimate and cultivate for 15 days. During the cultivation process, when the concentration of Chlorella reaches 1.0g / L, start to add unsterilized dairy farm wastewater into the medium , and every two days, 5% of the medium was replaced with an equal volume of aquaculture wastewater until the volume concentration of the aquaculture wastewater in the medium was 5%. ...

Embodiment 3

[0032] The wastewater from the dairy farm was collected, precipitated overnight, filtered through gauze, and the supernatant was taken for later use. Its water quality indicators are as follows: pH value is 8.18, total nitrogen (TN) concentration is 283.00mg / L, total phosphorus (TP) concentration is 115.90mg / L, chemical oxygen demand (COD) is 2593.00mg / L, suspended solids ( SS) was 1.74.

[0033] Take chlorella algae and inoculate it into BG-11 medium at a concentration of 0.09g / L, at an ambient temperature of 25°C and a light intensity of 800um m 2 ·S -1 , under the condition that the light time is 12 hours / day, domesticate and cultivate for 15 days. During the cultivation process, when the concentration of Chlorella reaches 1.2g / L, start to add unsterilized dairy farm wastewater to the medium , and every two days, 5% of the medium was replaced with an equal volume of aquaculture wastewater until the volume concentration of the aquaculture wastewater in the medium was 10%. ...

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Abstract

The invention relates to a method for culturing microalgae by utilizing aquaculture wastewater. The method comprises the following steps: 1) domesticating microalgae: inoculating microalgae into a culture medium for culturing, adding unsterilized aquaculture wastewater in a culture process and gradually increasing the adding rate of the aquaculture wastewater according to the growing number of themicroalgae; 2) activating the microalgae: adding the unsterilized aquaculture wastewater into the culture medium and then inoculating the domesticated microalgae into culture medium for performing amplification culture; and 3) culturing the microalgae: inoculating the activated microalgae into an unsterilized aquaculture wastewater solution and culturing under an outdoor condition, thereby realizing aquaculture wastewater purification and microalgae grease production. According to the method for culturing the microalgae by utilizing the aquaculture wastewater provided by the invention, the unsterilized aquaculture wastewater is adopted for culturing the microalgae under the outdoor condition, the aquaculture wastewater purification and the microalgae grease production can be realized at the same time and the method has the advantages of low cost and suitability for large-scale production.

Description

technical field [0001] The invention relates to a method for cultivating microalgae, in particular to a method for cultivating microalgae using aquaculture wastewater. Background technique [0002] Aquaculture wastewater is rich in nutrients such as nitrogen and phosphorus. A large amount of discharge will cause eutrophication of the water body. Algae and plankton will multiply wildly, causing the oxygen capacity of the water body to decrease, causing a large number of fish and other organisms to die, thereby destroying the ecology of the water body. balance. In recent years, dairy farms and other farms have concentrated large amounts of wastewater discharge, which has caused ecological crises in many water bodies, and their wastewater treatment methods need to be improved urgently. [0003] Chlorella has the advantages of high photosynthetic efficiency, fast growth rate, strong environmental adaptability and high oil yield, and has become the most potential biodiesel raw m...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/36C02F3/32C12P7/64C12R1/89
Inventor 陆伟东李少环
Owner SHAOGUAN COLLEGE
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