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Application of TPM2 proteins to preparation of rectal cancer prognosis evaluation kit, rectal cancer prognosis evaluation kit and rectal cancer prognosis evaluation method

A prognostic assessment, rectal cancer technology, applied in the biological field, can solve the problems of unclear molecular mechanism and lack of specific molecular targets for rectal cancer treatment

Inactive Publication Date: 2018-01-30
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Rectal cancer is one of the most threatening tumors to human health. The molecular mechanism of its occurrence is still unclear, and the treatment of rectal cancer lacks specific molecular targets. TPM2, as a very important tumor oncogene, is currently There is no literature report that TPM2 protein is related to the prognosis of rectal cancer or the metastasis of rectal cancer

Method used

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  • Application of TPM2 proteins to preparation of rectal cancer prognosis evaluation kit, rectal cancer prognosis evaluation kit and rectal cancer prognosis evaluation method
  • Application of TPM2 proteins to preparation of rectal cancer prognosis evaluation kit, rectal cancer prognosis evaluation kit and rectal cancer prognosis evaluation method
  • Application of TPM2 proteins to preparation of rectal cancer prognosis evaluation kit, rectal cancer prognosis evaluation kit and rectal cancer prognosis evaluation method

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Embodiment 1

[0042] A kit for postoperative prognosis evaluation of rectal cancer, the kit includes: human TPM2 monoclonal antibody or TPM2 polyclonal antibody for detecting the relative expression of TPM2 protein in rectal cancer tissue, immunohistochemical experiment reagent. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0043] The human TPM2 polyclonal antibody of the present invention is obtained by immunizing rabbits with the TPM2 protein whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0044] Immunohistochemical experiment reagent of the present invention comprises: Xylene, ethanol, 3%H 2 o 2 (aqueous solution), 3% BSA blocking solution (prepared in PBS), DAB chromogenic reagent, hematoxylin, horseradish peroxidase (for labeling secondary antibody), PB...

Embodiment 2

[0046] The application of TPM2 protein in the preparation of the postoperative prognosis evaluation kit of rectal cancer, the application is to use the kit of the present invention to evaluate the prognosis of rectal cancer postoperatively, the method is as follows:

[0047] (1) Take pathological specimens from patients with rectal cancer, and use human TPM2 monoclonal antibody or human TPM2 polyclonal antibody, as well as immunohistochemical reagents, for immunohistochemical staining;

[0048] Among them, the pathological specimens come from the biopsy tissue of rectal cancer patients or the pathological materials obtained during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0049] (a) Paraffin sections of rectal cancer tissues were prepared, and the oven was set at 60°C overnight.

[0050] (b) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[0051] ...

Embodiment 3

[0069] A postoperative tumor sample of rectal cancer was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the TPM2 immunohistochemical score of the cancer tissue was 0.632. After postoperative follow-up, it was found that the patient developed rectal cancer recurrence and metastasis 11 months after the operation, and died 19 months after the operation.

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Abstract

The invention provides application of TPM2 proteins to preparation of a rectal cancer prognosis evaluation kit, the rectal cancer prognosis evaluation kit and a rectal cancer prognosis evaluation method. The relative expression quantity of the TPM2 proteins in rectal cancer tissues is detected through an immunohistochemical method to judge the risks that rectal cancer patients suffer from relapseand metastasis. The application prompts that the TPM2 proteins can be also used for preparing protein molecule markers for judging prognosis of the rectal cancer patients and is of great guiding significance to postoperative monitoring and sequential therapy of the rectal cancer patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of TPM2 protein in the preparation of a kit for evaluating the prognosis of rectal cancer, the kit and the method for evaluating the prognosis of rectal cancer. Background technique [0002] Rectal cancer is a common malignant tumor in the gastrointestinal tract. The early symptoms are not obvious. With the increase of the cancer, symptoms such as changes in bowel habits, blood in the stool, diarrhea, alternating diarrhea and constipation, and local abdominal pain appear. In the late stage, anemia and weight loss are manifested. Wait for systemic symptoms. The morbidity and mortality of rectal cancer are second only to gastric cancer, esophageal cancer and primary liver cancer among digestive system malignancies. [0003] Rectal cancer is a cancer formed by abnormal growth of cells in the rectum. It is currently the most common digestive system cancer in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/577G01N33/531
Inventor 林尧王清水
Owner FUJIAN NORMAL UNIV
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