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Flos-loniceraefulvotomentosae extract, preparation containing extract and application of flos-lonicerae-fulvotomentosae extract to field of medicines

A technology of honeysuckle and extract, which is applied in the field of medicine, and can solve problems such as the extract of honeysuckle that has never been reported

Active Publication Date: 2018-02-02
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art has never reported the Lonicera tomentosa extract

Method used

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  • Flos-loniceraefulvotomentosae extract, preparation containing extract and application of flos-lonicerae-fulvotomentosae extract to field of medicines
  • Flos-loniceraefulvotomentosae extract, preparation containing extract and application of flos-lonicerae-fulvotomentosae extract to field of medicines
  • Flos-loniceraefulvotomentosae extract, preparation containing extract and application of flos-lonicerae-fulvotomentosae extract to field of medicines

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] Preparation and content determination of sample solution: Take an appropriate amount of extract, weigh it accurately, put it in a volumetric flask, dissolve it with 70% methanol (v / v), shake it well, filter it with a microporous membrane (0.45 μm), and take the continuation The filtrate was subjected to HPLC-ELSD analysis according to the above-mentioned HPLC chromatographic conditions, and the contents of the above three monomers in each extract were calculated by using the external standard method.

[0034] The chromatographic separation effect is as figure 1 as shown, figure 1 Middle A is the chromatogram of Decaisoside E, fulvotomentoside B (fulvotomentoside B) and dipsacoside B (dipsacoside B) mixed reference substance solution, figure 1 Middle B is the chromatogram of the 70% methanol total extract of Lonicerae flavus Lonicerae medicinal material, and the chromatographic separation effect is excellent.

Embodiment 1

[0035] Embodiment 1: the preparation of the extract of honeysuckle

[0036] Preparation method one5kg of dry Lonicera chinense medicinal material, reflux extraction with 70% ethanol for 3 times, 2h each time, filter, combine the filtrate, reclaim the solvent under reduced pressure to obtain extractum (get partly dried to obtain total extract 1), add appropriate amount of water to suspend , filter, and load the sample on D101 macroporous resin, start gradient elution from 10%, 30%, 50%, 70%, 90% ethanol, and monitor whether the eluate contains Decaisoside E, Honeysuckle saponin B or Dipsacus saponin B, any of the three appear in the eluent and start to collect until all three are eluted from the resin and stop collecting. Concentrate and dry this part of the eluent to obtain the extract of Lonicera tomentosa Object 1.

[0037] Preparation method two 5kg of dry Lonicera chinensis medicinal material, soak 24h with 90% ethanol, filter, collect the filtrate, reclaim the solven...

Embodiment 2

[0044] Example 2: The prevention and treatment effect of Lonicera chrysalis extract on stroke

[0045] 1. Effect on survival time of mice after cerebral ischemia induced by bilateral ligation of common carotid artery and vagus nerve

[0046] 60 Kunming mice were randomly divided into 6 groups according to body weight and sex: model group and Lonicera tomentosa extract 1-5 administration group. The mice in the administration group were given the extract containing 50 mg / kg / d of three kinds of total saponins by intragastric administration respectively, and the mice in the model group were given the same volume of solvent 0.5% CMC-Na by intragastric administration for 2 consecutive weeks. 0.5 hours after the last gavage, the mice were superficially anesthetized with ether, and the bilateral common carotid arteries and vagus nerves were quickly ligated. The survival time of the mice was observed and recorded, and the differences in the survival time of the mice in each group were ...

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Abstract

The invention discloses a flos-lonicerae-fulvotomentosae extract, a preparation containing the extract and application of the flos-lonicerae-fulvotomentosae extract to the field of medicines. The flos-lonicerae-fulvotomentosae extract is obtained by using flos lonicerae fulvotomentosae as a raw material through extraction and enrichment, wherein the sum of the mass-percent contents of DecaisosideE, fulvotomentoside B and dipsacoside B is not less than 50 percent. Known according to embodiments of a preparation method and pharmacodynamic embodiments, which are provided by the invention, the flos-lonicerae-fulvotomentosae extract which is provided by the invention and in which the sum of the mass-percent contents of the Decaisoside E, the fulvotomentoside B and the dipsacoside B is not lessthan 5o percent has multiple pharmacologic effects, can be used for developing to form medicines for preventing and treating different diseases; the pharmacologic intensity of the flos-lonicerae-fulvotomentosae extract is basically equivalent to that of a flos-lonicerae-fulvotomentosae total extract; however, main pharmacodynamic components are more explicit; the dosage of a patient can be greatly decreased; meanwhile, the quality control is carried out conveniently and the modernization requirement of the traditional Chinese medicine is met.

Description

technical field [0001] The invention belongs to the field of medicine and relates to an extract, specifically a honeysuckle extract, a preparation containing the extract and an application in the field of medicine. Background technique [0002] Lonicera chrysalis is the dry flower bud or flower with the first bloom of L.fulvotomentosa Hsu et S.C.Cheng, a plant of Lonicaceae. It was first published in Volume 17 of "Journal of Plant Taxonomy" in 1979, and it was a local drug in Guizhou Province before 2010. According to the standard, the 2010 edition of the "Chinese Pharmacopoeia" defines Lonicera chrysanthemum as one of the base plants of the medicinal material of mountain silver flower. [0003] Although Lonicera chrysalis appears in many traditional Chinese medicine prescriptions, it is well known that the overall use of Chinese medicinal materials not only requires a large amount of medicine, which increases the burden of taking medicine for patients, but also has the prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P19/10A61P9/10A61P1/16A61P31/04A61P31/12A61P29/00A61P39/06
CPCA61K36/355
Inventor 齐炼文李萍黄丰青
Owner CHINA PHARM UNIV
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