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Rapid tillering propagation medium for tissue culture of arundo donax

A medium and tissue culture technology, applied in the field of reed bamboo tissue culture tiller fast propagation medium, can solve problems such as unstable survival rate

Inactive Publication Date: 2018-02-06
王晓翔 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With 2-4 stem cuttings, the survival rate is unstable, between 40% and 80%, and the survival rate of single-node cuttings is only about 20%.

Method used

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  • Rapid tillering propagation medium for tissue culture of arundo donax
  • Rapid tillering propagation medium for tissue culture of arundo donax
  • Rapid tillering propagation medium for tissue culture of arundo donax

Examples

Experimental program
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Embodiment Construction

[0021] Primary culture of explants: Insert the explants (stem nodes) that have been sterilized obliquely or vertically into glass bottles with prepared medium for culture. Two different mediums can be used for primary culture, respectively. for:

[0022] a. Tiller medium (1 / 2MS or MS+0.1-0.5mg / LNAA+5mg / L IBA+15g sucrose+7g agar), after inoculation on it, axillary bud sprouting was observed after 3-4 days, and leaf bud base was observed after 6-20 days After the root system sprouts, a complete plantlet can be formed. At this time, the plant is connected to the thicker stem segment, that is, the original explant.

[0023] b. Induced cluster shoot medium (1 / 2MS or MS+1-4mg / L 6-BA+0.1mg / LNAA+5mg / L IBA+15g sucrose+7g agar), about 10 days will grow clusters without roots Buds, 3-15 clustered buds or seedlings with roots will grow in about 20-30 days.

[0024] Proliferation of seedlings:

[0025] a. The plants with 2-5 stem nodes cultivated in the first generation on the tillering...

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Abstract

The invention relates to a tissue culture and rapid propagation medium for arundo donax. Basic MS and a plant growth regulator are combined to form a medium formula. With adoption of the tissue culture and rapid propagation medium, axillary bud stem sections of superior plants are taken as explants, the whole sterile culture process is simple and feasible, and one medium can be even obtained by simplification, namely, the medium for one-step seedling forming and rapid tillering propagation. Subculture, stem node induced rooting and seedling forming and root section tillering proliferation areexecuted synchronously in the tillering medium, so that the growing period of tissue culture seedlings of the arundo donax is shortened, and industrial production is facilitated.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation medium for plants, in particular to a tissue culture and tiller rapid propagation medium for reed bamboo. Background technique [0002] Phyllostachys genus is a perennial herb with tall perennial roots, shaped like a reed. The rhizomes are short, strong, multi-branched, the leaves are broadly lanceolate, the panicles are terminal, and the spikes are broom-shaped. The flowering and fruiting period is from September to December. It is the main waterside landscape plant. Reed bamboo is a very effective corn substitute and is an annual plant with great potential for increasing yields in terms of biomass, maintaining productivity for 10-15 years and adapting to marginal soils. In addition, Luzhu has another great advantage, that is, it will not take away the fertility of the soil while producing crops. The stalk is the reed in a wind instrument. The stem fiber is long, the aspect ratio is l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 王晓翔吴慎杰马国杰张换样李静李盛
Owner 王晓翔
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