Dendritic macromolecule modified gold nano particles as well as preparation method and application thereof

A gold nanoparticle and dendritic technology, which is applied in the field of dendrimer-modified gold nanoparticle and its preparation, and achieves the effects of reducing biological toxicity, improving stability and easy structure.

Active Publication Date: 2018-02-06
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, it has not been found that azide chitosan can be coupled with alkyne-containing dendritic polyamidoamines into a biocompatible and degradable polyamide-amine using click chemistry without adding other reducing agents. Chitosan-b

Method used

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  • Dendritic macromolecule modified gold nano particles as well as preparation method and application thereof
  • Dendritic macromolecule modified gold nano particles as well as preparation method and application thereof
  • Dendritic macromolecule modified gold nano particles as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] (1) Chitosan modified with azide group (CS-N 3 )Synthesis

[0063] Add azidoacetic acid (N 3 -CH2-COOH) was dissolved in NN-dimethylformamide (DMF), and then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-Hydroxysuccinimide (NHS) is activated for 30 minutes to obtain the azide acetic acid mixed solution; after the activation, chitosan (CS molecular weight is 2000, deacetylation degree 85%) is dissolved in pure water, and then slowly added to the above In the azide acetic acid mixed solution, the reaction was carried out at room temperature 35°C for 12 hours. The product was dialyzed in pure water for 2 days and freeze-dried to obtain the product azide chitosan; wherein, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride The molar ratio of salt (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid is 1:1.5:1.5:2; the N,N-dimethylformamide is in 10 ml Add 5g hydrazoic acid; the pure water is calculated by adding 5g chi...

Embodiment 2

[0067] (1) Chitosan modified with azide group (CS-N 3 )Synthesis

[0068] Add azidoacetic acid (N 3 -CH2-COOH) was dissolved in NN-dimethylformamide (DMF), and then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-Hydroxysuccinimide (NHS) is activated for 4h to obtain the azide acetic acid mixed solution; after activation, chitosan (CS molecular weight is 20000, deacetylation degree 40%) is dissolved in pure water, and then slowly added to the above In the azide acetic acid mixed solution, react at room temperature for 24 hours. The product was dialyzed in pure water for 3 days and freeze-dried to obtain the product azide chitosan. Wherein, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:10:10:10; the N,N-dimethylformamide is calculated by adding 1g hydrazoic acid per 10ml; the pure water is calculated by adding 0.5g chitosan per 10ml Sugar meter. ...

Embodiment 3

[0072] (1) Chitosan modified with azide group (CS-N 3 )Synthesis

[0073] Add azidoacetic acid (N 3 -CH2-COOH) was dissolved in NN-dimethylformamide (DMF), and then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-Hydroxysuccinimide (NHS) is activated for 2h to obtain azide acetic acid mixed solution; after activation, chitosan (CS molecular weight is 10000, deacetylation degree 60%) is dissolved in pure water, and then slowly added to the above In the azide acetic acid mixed solution, react at room temperature for 16 hours. The product was dialyzed in pure water for 2 days and freeze-dried to obtain the product azide chitosan. Wherein, the chitosan, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), N-hydroxysuccinimide (NHS) and azidoacetic acid The molar ratio is 1:5:5:5; the N,N-dimethylformamide is calculated by adding 3g hydrazoic acid per 10ml; the pure water is calculated by adding 3g chitosan per 10ml meter.

[0074] (2) Syn...

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Abstract

The invention discloses dendritic macromolecule modified gold nano particles as well as a preparation method and application thereof. The method comprises the following steps: performing azide modification on chitosan, and synthesizing glycan coupled dendritic polyamide-amine (CS-PAMAM) with alkynyl-containing dendritic polyamide-amine through a click reaction; and synthesizing gold nano-particleswith uniform grain size in one stop by using CS-PAMAM as a stabilizer and a reducer in one stop under the condition of not adding other reducer to obtain dendritic macromolecule modified gold nano particles having relatively small grain size. The gold nano particles have high stability and good biocompatibility besides favoring reduction of product cytotoxicity, massive amine functional groups are contained on the surface, target property is given to the gold nano particles, and an important application prospect is showed in gene transfer and tumor diagnosis integration. The dendritic macromolecule modified gold nano particles have excellent transfection, can be used as a medicine controlled-release vector, and has a potential application value in the field of medicine co-transfer.

Description

Technical field [0001] The invention belongs to the field of biomedical engineering materials, and particularly relates to a dendritic macromolecule modified gold nanoparticle, and a preparation method and application thereof. Background technique [0002] Gene therapy is currently an effective method for the treatment of cancer and congenital immune system diseases. The key to the implementation of this technology is to select a suitable gene vector and gene introduction method, so that the gene can be safely, efficiently, controlled and stable expression in the cell. Viral vectors are limited in clinical applications due to their high risk, immune response, and inability to produce on a large scale. Therefore, liposomes and other cationic polymers with high transfection efficiency have been paid more and more attention. [0003] Gold nanoparticles are one of the first major categories of metal nanoparticles, which refer to gold nanoparticles with a size ranging from 1 nm to 100 ...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K48/00A61K41/00A61K49/00A61P35/00
CPCA61K41/0052A61K48/0041A61K48/0091A61K49/00
Inventor 俞思明李国巍马栋薛巍
Owner JINAN UNIVERSITY
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