Cosmetic composition comprising slime of snail fed with berry fruits and preparation method thereof
A technology of cosmetic composition and snail mucus, which is applied in the direction of cosmetic preparations, medical preparations containing active ingredients, cosmetics, etc., can solve problems such as inability to play, and achieve the effects of reducing skin pressure, preventing skin aging, and reducing active oxygen
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Embodiment 1
[0075] Example 1 of preparing snail mucus filtrate from snails fed berries
[0076] Example 1 of the preparation of snail mucus filtrate from berry-fed snails according to the present invention was prepared by the following preparation steps.
[0077] (S10): Growing snails on berry seedlings at a temperature of 25°C and a humidity of 65%.
[0078] (S20): After washing the snail with purified water and putting the snail into the net, snail slime was collected for 5 hours.
[0079] (S30): The snail mucus filtrate is obtained by filtering the snail mucus through a filter device equipped with a porous filter having a pore size of 250 nanometers (nm), and the snail mucus filtrate is stored in a low-temperature refrigerator maintained at 5°C, To prevent the snail mucus filtrate from going bad.
Embodiment 2
[0080] Example 2 of preparing snail mucus filtrate from snails fed berries
[0081] Example 2 of the preparation of snail mucus filtrate from berry-fed snails according to the present invention was prepared by the following preparation steps.
[0082] (S10): Growing snails on berry seedlings at a temperature of 25°C and a humidity of 65%.
[0083] (S20): After washing the snail with purified water and putting the snail into the net, snail slime was collected for 5 hours.
[0084] (S30): Filtrating the snail mucus through a filter device equipped with a porous filter having a pore size of 250 nanometers (nm) to obtain a snail mucus filtrate.
[0085] (S40): After injecting the collagen decomposition inhibitor or the elastin decomposition inhibitor into the snail mucus filtrate, store the prepared snail mucus filtrate in a low-temperature refrigerator kept at 5°C.
experiment example 1
[0096] the measurement of the antioxidant effect of the prepared snail mucus filtrate in embodiment 1 and embodiment 2 and comparative example 1 and comparative example 2
[0097] As a verification test of the skin aging improving effect, a free radical scavenging test and an active oxygen scavenging test were performed in order to confirm the antioxidant effect of the snail mucus filtrate prepared in Example 1 and Example 2 and Comparative Example 1 and Comparative Example 2.
[0098] The free radical scavenging test was carried out by a verification test protocol under the following conditions: the absorbance of the stabilized DPPH exhibited a maximum absorbance at 540 nm, and the absorbance at the above-mentioned wavelength of 540 nm decreased as the free radical scavenging rate increased, That is, DPPH decreases as free radicals are scavenged by the sample and turn purple to transparent.
[0099] The snail mucus filtrate prepared in the above-mentioned Example 1 and Examp...
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