Aquaculture water cleaning agent and preparation method thereof
A technology of aquaculture and purification agent, applied in biochemical equipment and methods, chemical instruments and methods, water/sewage treatment, etc., can solve problems affecting fish enzyme activity, slow growth of fish and shrimp, and breeding biological toxicity, etc., to achieve Effects of meat quality and flavor improvement, survival rate improvement, and water quality improvement
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Embodiment 1
[0065] A kind of preparation method of photosynthetic bacteria agent is as follows:
[0066] a), seed liquid preparation
[0067] On a sterile operating table, inoculate Rhodopseudomonas palustris into the seed medium, and cultivate the bacteria in a shaker at 30°C and 180rpm / min under the light of 2400lux incandescent lamp until the concentration of the bacteria reaches 10 8 CFU / mL, to obtain Rhodopseudomonas palustris seed solution;
[0068] b), fermentation culture
[0069] Add 5mL of Rhodopseudomonas palustris seed liquid to the fermentation medium, cultivate it in a shaker at 30°C and 180rpm / min under the light of a 2400lux incandescent lamp, and detect the number of bacteria in the fermentation bacteria until the number of bacteria reaches 10 10 When the CFU / mL is reached, the fermentation is stopped to obtain the photosynthetic bacteria agent.
[0070] The pH of the seed medium is 7.0, containing the following substances: 2 g of ammonium chloride, 5 g of sodium aceta...
Embodiment 2
[0073] A kind of preparation method of photosynthetic bacteria agent is as follows:
[0074] a), seed liquid preparation
[0075] On a sterile operating table, inoculate Rhodospirillum rubrum into the seed culture medium, and cultivate the bacteria in a shaker at 30°C and 180rpm / min under the light of 2400lux incandescent lamp until the concentration of the bacteria reaches 10 8 CFU / mL, to obtain Rhodospirillum rubrum seed solution;
[0076] b), fermentation culture
[0077] Add 5mL of Rhodospirillum rubrum seed solution to the fermentation medium, culture in a shaker at 30°C, 180rpm / min, and under a 2400lux incandescent lamp to detect the number of bacteria in the fermentation bacteria, and wait until the number of bacteria reaches 10 10 When the CFU / mL is reached, the fermentation is stopped to obtain the photosynthetic bacteria agent.
[0078] The pH of the seed medium is 7.0, containing the following substances: 2 g of ammonium chloride, 5 g of sodium acetate, 0.1 g of ...
Embodiment 3
[0081] A kind of preparation method of photosynthetic bacteria agent is as follows:
[0082] a), seed liquid preparation
[0083] On a sterile operating table, Rhodococcus yunnanensis was inoculated into the seed medium, and cultured in a shaker at 30°C and 180rpm / min under 2400lux incandescent light until the concentration of the bacteria reached 10 8 CFU / mL to obtain Rhodococcus yunnanensis seed solution;
[0084] b), fermentation culture
[0085] Add 5mL of Rhodococcus yunnanensis seed solution to the fermentation medium, and culture it in a shaker at 30°C and 180rpm / min under a 2400lux incandescent lamp to detect the number of bacteria in the fermentation bacteria, and wait until the number of bacteria reaches 10 10 When the CFU / mL is reached, the fermentation is stopped to obtain the photosynthetic bacteria agent.
[0086] The pH of the seed medium is 7.0, containing the following substances: 2 g of ammonium chloride, 5 g of sodium acetate, 0.1 g of magnesium chloride,...
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