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A root-knot nematode-killing Pseudomonas berry sneb811, metabolites and applications

A sneb811, Pseudomonas technology, applied in the direction of nematicides, applications, biocides, etc., to achieve good application prospects, strong poisonous killing effect

Active Publication Date: 2021-02-26
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And utilize Pseudomonas berry or the metabolite of Pseudomonas berry to process tomato root, the method for preventing and treating root-knot nematode incognita has not yet been reported

Method used

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  • A root-knot nematode-killing Pseudomonas berry sneb811, metabolites and applications
  • A root-knot nematode-killing Pseudomonas berry sneb811, metabolites and applications
  • A root-knot nematode-killing Pseudomonas berry sneb811, metabolites and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0031] In this embodiment, a root-knot nematode metabolite prepared by Pseudomonas berry Sneb811, the preparation method is as follows:

[0032] After activating the test tube strain of Pseudomonas berry Sneb811, inoculate it into a test tube culture medium for seed culture. The formula of the test tube culture medium is beef extract peptone medium, namely ingredients: beef extract 3g, yeast extract 1g, Peptone 10g, sucrose 10g, agar 17g, add distilled water to 1000mL. The seed culture conditions are as follows: culture at 28°C for 3 days to obtain a test tube seed liquid.

[0033] Then the obtained seed solution was inoculated in a 250mL Erlenmeyer flask (150mL per bottle) liquid fermentation medium to carry out fermentation culture to obtain root-knot nematode metabolites. The formula of the liquid fermentation medium is: in terms of mass percentage, 0.1% of yeast extract, 0.5% of peptone, 0.3% of beef extract, 1% of sucrose, the rest is distilled water, and the pH is 6.8-7...

Embodiment 2

[0035] In this embodiment, a root-knot nematode metabolite prepared by Pseudomonas berry Sneb811, the preparation method is as follows:

[0036] After activating the test tube strain of Pseudomonas berry Sneb811, inoculate it into a test tube culture medium for seed culture. The formula of the test tube culture medium is beef extract peptone medium, namely ingredients: beef extract 3g, yeast extract 1g, Peptone 10g, sucrose 10g, agar 20g, add distilled water to 1000mL. The seed culture conditions are as follows: culture at 28°C for 3 days to obtain a test tube seed liquid.

[0037] Then the obtained seed solution was inoculated in a 250mL Erlenmeyer flask (150mL per bottle) liquid fermentation medium to carry out fermentation culture to obtain root-knot nematode metabolites. The formula of the liquid fermentation medium is: in terms of mass percentage, 0.2% of yeast extract, 0.6% of peptone, 0.4% of beef extract, 2% of sucrose, the rest is distilled water, and the pH is 6.8-7...

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Abstract

The invention discloses a kind of Pseudomonas berry Sneb811 poisonous to kill tomato root-knot nematode incognita. General Microbiology Center of the Culture Collection Management Committee, the preservation number is CGMCC No.14018, and the preservation date is April 12, 2017. The metabolites of the strain after conventional liquid fermentation and culture can develop biological agents with strong poisonous effect on root-knot nematode incognita, which has strong poisonous effect on root-knot nematode incognita, and can effectively kill its oocysts It has obvious inhibitory effect, is a potential biocontrol bacterium, and has a good application prospect in the prevention and treatment of crop root-knot nematode damage.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to Pseudomonas berry Sneb811 for killing root-knot nematodes, metabolites and applications. Background technique [0002] Root-knot nematode (Meloidogyne incognita) causes significant yield loss in vegetables around the world, is difficult to control, and makes plants more susceptible to complex infections of other fungi and bacteria. Effective, highly toxic and high-residue chemical nematicides pose potential threats to soil ecosystems, groundwater, and human health. Currently, dissatisfaction with chemical nematicides has spurred research into pollution-free alternative control strategies. The development of alternatives to highly toxic and high residue chemical nematicides is one of the major future issues. [0003] There are many types and quantities of soil microorganisms, which are closely related to soil nematodes and crop roots. Among them are man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P5/00C12R1/38
CPCA01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 陈立杰赵惠段玉玺赵丹朱晓峰王媛媛
Owner SHENYANG AGRI UNIV