Microbial fertilizer prepared from yeast fermentation broth and preparation method of microbial fertilizer
A microbial fertilizer and yeast fermentation technology, applied in the direction of organic fertilizers, fertilizer forms, fertilizer mixtures, etc., can solve the problems of yeast wastewater restricting the yeast industry, and achieve the effects of reducing labor intensity, convenient storage, and reducing the frequency of fertilization
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Embodiment 1
[0041] A kind of microbial fertilizer that utilizes yeast fermented liquid to prepare, described microbial fertilizer is prepared by the method comprising the following steps:
[0042] A. Activate yeast: inoculate the yeast parent species on the slant activation medium and culture it statically at 28°C for 36 hours to obtain activated yeast;
[0043] The slant activation medium is made of the following components in percentage by weight: molasses 5%, peptone 1%, yeast extract 2%, disodium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, agar 2.5%, and the balance is water, pH 7.0;
[0044] B. Cultivate seed bacteria liquid: Inoculate the activated yeast obtained in step A into the sterilized medium, which accounts for 45% of the volume of the fermentation vessel; at 28°C, the fermentation pressure is 0.025MPa, and the ventilation rate is 0.85 V / v·min, stirring speed is 10 hours under the condition of 140rpm cultivation, obtains the seed bacterial liquid;
[0045...
Embodiment 2
[0052] A kind of microbial fertilizer that utilizes yeast fermented liquid to prepare, described microbial fertilizer is prepared by the method comprising the following steps:
[0053] A. Activating yeast: Inoculate the yeast parent species on the slant activation medium and culture it statically at 30°C for 24 hours to obtain activated yeast;
[0054] The slant activation medium is made of the following components in percentage by weight: molasses 10%, peptone 0.5%, yeast extract 5%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.5%, agar 1.5%, and the balance is water with a pH of 7.5;
[0055] B. Cultivate seed bacteria liquid: inoculate the activated yeast obtained in step A into the sterilized medium, which accounts for 25% of the volume of the fermentation vessel; at 30°C, the fermentation pressure is 0.02MPa, and the ventilation rate is 1.05 v / v·min, the stirring speed is 120rpm under the condition of cultivating for 12 hours to obtain the seed bact...
Embodiment 3
[0063] A kind of microbial fertilizer that utilizes yeast fermented liquid to prepare, described microbial fertilizer is prepared by the method comprising the following steps:
[0064] A. Activating yeast: Inoculate the yeast parent species on the slant activation medium and culture it statically at 29°C for 30 hours to obtain activated yeast;
[0065] The slant activation medium is made of the following components in percentage by weight: molasses 7.5%, peptone 0.75%, yeast extract 3.5%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.3%, agar 2%, and the balance is water with a pH of 7.2;
[0066] B. Cultivate seed bacteria liquid: Inoculate the activated yeast obtained in step A into the sterilized medium, which accounts for 35% of the volume of the fermentation vessel; at 29°C, the fermentation pressure is 0.02MPa, and the ventilation rate is 0.95 v / v min, the stirring speed is 130rpm under the condition of cultivating for 11 hours to obtain the seed ba...
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