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Preparation method of high-purity D-psicose

A allulose, high-purity technology, applied in the field of bioengineering, can solve the problems of high manufacturing cost, limited application, and difficulty in effectively exerting product characteristics, so as to reduce costs, reduce production costs, and improve conversion efficiency.

Inactive Publication Date: 2018-02-16
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current high price of D-psicose is mainly due to the high manufacturing cost, which greatly limits its application in the downstream food and health care products, and it is difficult to effectively utilize the characteristics of the product itself.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A preparation method of high-purity D-psicose, comprising the steps of:

[0051] (1) Take Bacillus subtilis (Bacillus subtilis) BLCY-005, inoculate it in the seed culture medium, and carry out seed cultivation at a temperature of 35°C and 220 rpm for 8 hours to obtain a seed solution;

[0052] Described seed culture medium component is as follows, is weight percent:

[0053] Peptone 1.0%, corn steep liquor powder: 0.5%, glycerin: 1.0%, balance water, pH7.0;

[0054] (2) Get the seed liquid obtained in step (1), inoculate it in the fermentation medium according to the inoculum size of 2% by volume, at a temperature of 35°C, pH7.0, tank pressure 0.05Mpa, ventilation ratio 2.0vvm, rotating speed 300rpm , under the condition of 35% dissolved oxygen, fermented and cultivated for 36 hours to obtain a fermented liquid;

[0055] Described fermenting medium, component is as follows, is mass percent:

[0056] Corn steep liquor powder 5%, glucose 1%, naphthalene acetic acid 0.1...

Embodiment 2

[0072] A preparation method of high-purity D-psicose, comprising the steps of:

[0073] (1) Take Bacillus subtilis (Bacillus subtilis) BLCY-005, inoculate it in the seed culture medium, and carry out seed cultivation at a temperature of 35°C and 220 rpm for 8 hours to obtain a seed solution;

[0074] Described seed culture medium component is as follows, is weight percent:

[0075] Peptone 1.0%, corn steep liquor powder: 0.5%, glycerin: 1.0%, balance water, pH7.0;

[0076] (2) Get the seed liquid obtained in step (1), inoculate it in the fermentation medium according to the inoculum size of 2% by volume, at a temperature of 35°C, pH7.0, tank pressure 0.05Mpa, ventilation ratio 2.0vvm, rotating speed 300rpm , under the condition of 35% dissolved oxygen, fermented and cultivated for 36 hours to obtain a fermented liquid;

[0077] Described fermenting medium, component is as follows, is mass percent:

[0078] Corn steep liquor powder 5%, glucose 1%, naphthalene acetic acid 0.1...

Embodiment 3

[0094] A preparation method of high-purity D-psicose, comprising the steps of:

[0095] (1) Take Bacillus subtilis (Bacillus subtilis) BLCY-005, inoculate it in the seed culture medium, and carry out seed cultivation at a temperature of 35°C and 220 rpm for 8 hours to obtain a seed solution;

[0096] Described seed culture medium component is as follows, is weight percent:

[0097] Peptone 1.0%, corn steep liquor powder: 0.5%, glycerin: 1.0%, balance water, pH7.0;

[0098](2) Get the seed liquid obtained in step (1), inoculate it in the fermentation medium according to the inoculum size of 2% by volume, at a temperature of 35°C, pH7.0, tank pressure 0.05Mpa, ventilation ratio 2.0vvm, rotating speed 300rpm , under the condition of 35% dissolved oxygen, fermented and cultivated for 36 hours to obtain a fermented liquid;

[0099] Described fermenting medium, component is as follows, is mass percent:

[0100] Corn steep liquor powder 5%, glucose 1%, naphthalene acetic acid 0.1%...

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Abstract

The invention relates to a preparation method of high-purity D-psicose. The method comprises the following steps: (a) preparing a D-psicose epimerase crude enzyme preparation; (b) immobilizing the crude enzyme preparation prepared in the step (a) and glucose isomerase; (c) preparing a mixed solution containing D-psicose, fructose and glucose; (d) decolorizing, filtering and hybridizing the mixed solution prepared in the step (c) to obtain a D-psicose solution and a mixed solution of the fructose and the glucose; (e) performing chromatographic separation, concentration, crystallization or drying on the D-psicose solution prepared in the step (d) to obtain D-psicose.

Description

technical field [0001] The invention relates to a preparation method of high-purity D-psicose, which belongs to the technical field of bioengineering. Background technique [0002] D-Psicose is an important rare sugar, which exists in very small amounts in sugarcane molasses, dried fruits, sugar products, wheat and stingray plants in nature. As a sweetener, the sweetness of D-psicose is equivalent to 70% of fructose, the energy is only 0.3% of sucrose, and almost no heat is produced after consumption. It is a sweetener that can be eaten by diabetics in the true sense. New research has found that after eating D-psicose, not only will blood sugar not rise, but it can also effectively regulate blood sugar, reduce energy intake, and reduce abdominal fat accumulation. Its unique functions and potential medical Value has attracted the attention of many research scholars and is increasingly popular with consumers. In 2011, the FDA approved the sweetener D-psicose as a food additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/90C12P19/24C12P19/02C12R1/125
CPCC12N9/90C12P19/02C12P19/24C12Y501/03
Inventor 李方华窦光朋邵先豹刘杰刘双双杜倩张明站
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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