Visual detection method for clenbuterol hydrochloride
A clenbuterol hydrochloride and detection method technology, which is applied in the field of visual detection of clenbuterol hydrochloride, can solve the problems of non-toxic damage or loss, and achieve the effects of easy preparation, good linearity and high sensitivity
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[0035] The present invention will be described in further detail below in conjunction with accompanying drawing and specific embodiment, and unmarked implementation condition is the routine condition in this industry.
[0036] 1. Synthesis of thiolated clenbuterol hydrochloride aptamer and thiolated clenbuterol hydrochloride aptamer complementary oligonucleotide single strand (purchased from Shanghai Sangon Bioengineering Co., Ltd.).
[0037] The sequence of the thiolated clenbuterol hydrochloride aptamer is: 5'SH-AGC AGC ACA GAG GTC AGA TGT CATCTG AAG TGA ATG AAG GTAAAC ATT ATT TCA TTAACACCT ATG CGT GCT ACC GTG AA-3'.
[0038] The complementary oligonucleotide single-stranded sequence of thiolated clenbuterol hydrochloride aptamer is: 5'SH-TTC ACG GTA GCACGC ATAGGT GTT AAT GAA ATAATG TTT A-3'.
[0039] 2. Preparation of nano gold.
[0040] (1) Soak relevant containers with aqua regia prepared by concentrated hydrochloric acid and concentrated nitric acid with a volume ratio ...
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