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Drug loading system of tetrahedral structure and pharmaceutical application

A tetrahedral, drug-carrying technology, applied in the field of biomedicine, can solve the problems of difficult metabolism, limited application, poor biocompatibility, etc.

Active Publication Date: 2018-02-23
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to its potential pathogenicity, immunogenicity and other shortcomings, the application of viral vectors is limited; non-viral vectors have problems such as poor biocompatibility, difficult metabolism, and easy accumulation in the body to produce toxicity.

Method used

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  • Drug loading system of tetrahedral structure and pharmaceutical application
  • Drug loading system of tetrahedral structure and pharmaceutical application
  • Drug loading system of tetrahedral structure and pharmaceutical application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Instruments and Equipment

[0061]

[0062]

[0063] cell line

[0064] Human nasopharyngeal carcinoma cell line SUNE-1 5-8F was purchased from Sun Yat-Sen University Cell Bank.

[0065] main reagent

[0066] The DNA sequence was purchased from Shanghai Sangon Bioengineering Co., Ltd., and the purification level was ULTRAPAGE or HPLC. The DNA sequence is shown in Table 1.

[0067] Table 1 DNA sequence

[0068]

[0069]

[0070] Note: * refers to the modified site

[0071] 1) RPMI 1640 medium Gibco, USA

[0072] 2) Fetal bovine serum Gibco, USA

[0073] 3) Trypsin Gibco, USA

[0074] 4) CCK-8 Kit Solarbio, China

[0075] 5) PBS powder Google Bio, China

[0076] 6) Dimethyl sulfoxide (DMSO) Sigma, USA

[0077] 7) Ripa Lysate Biyuntian, China

[0078] 8) Tris-base Solarbio, China

[0079] 9) MgCl 2 ·5H 2 O Biosharp, China

[0080] 10) Penicillin Gibco, USA

[0081] 11) Streptomycin Gibco, USA

[0082] 12) Gel preparation kit Boster, China

[00...

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Abstract

The invention provides a drug loading system of a tetrahedral structure. The structure of the drug loading system is Aptamer-AS1411-DNA. The structure of the drug loading system is Aptamer1-AS1411-DNA, and particularly the structure is formed by simultaneously connecting one Aptamer1 and three AS1411 onto a DNA tetrahedral body in an alkali complementary pairing way. The drug loading system is applied to a nasopharynx cancer drug, and is particularly applied to a drug targeting CD44<+> nasopharynx cancer cells; and meanwhile, the drug loading system is applied to a drug inhibiting the multiplication of nasopharynx cancer 5-8F cells.

Description

technical field [0001] The invention provides the design, preparation and pharmaceutical use of a drug-carrying system with a tetrahedral structure, belonging to the technical field of biomedicine. Background technique [0002] Nasopharyngeal carcinoma (NPC) is currently the most common head and neck malignancy. Worldwide, its main incidence areas are southern China, Southeast Asia, and central, eastern and southern Africa. Especially, the Cantonese population in southern China has the highest incidence rate, reaching 25-30 / 100,000; while the incidence rate of NPC in most western countries is relatively low. Low. NPC is relatively sensitive to radiation, and some patients with early NPC can be cured after radiotherapy alone; however, even if advanced (Ⅲ,Ⅳ) NPC is treated with radiotherapy and chemotherapy, the 5-year survival rate is still hovering around 10% to 40%. not fundamentally improved. There are still huge challenges in the treatment of refractory NPC and metasta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K47/69A61K31/7088A61P35/00
CPCA61K31/7088
Inventor 孙丽丹许新华袁程许璐郭蓉
Owner CHINA THREE GORGES UNIV
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