Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multilayer high-intensity artificial endocranium containing cell factors and preparation method of multilayer high-intensity artificial endocranium

An artificial dura mater and cell technology, applied in medical science, non-woven fabrics, filament/thread forming, etc., can solve the problems of low strength, insufficient bonding strength, unfavorable cell attachment, etc., to increase the contact probability and improve the binding force Effect

Active Publication Date: 2018-02-23
李瑞锋
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are the following disadvantages: 1) The outer layer of the meninges is a colloidal structure, not a nanofiber scaffold, which is not conducive to cell attachment, and the repair cycle of the meninges is long. 2) Cell growth factors are dissolved in the colloid, and only the cell growth factors on the surface are in contact with the cells. Limited promotion of cell production
However, the two layers of this product are prepared by the roller oriented spinning process, and the two layers of the meninges are oriented nanofibers. The strength of this fiber film is much lower than that of the non-oriented fiber film.
At the same time, the artificial dura mater has only two layers, and the bonding strength between the two layers is not enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multilayer high-intensity artificial endocranium containing cell factors and preparation method of multilayer high-intensity artificial endocranium
  • Multilayer high-intensity artificial endocranium containing cell factors and preparation method of multilayer high-intensity artificial endocranium
  • Multilayer high-intensity artificial endocranium containing cell factors and preparation method of multilayer high-intensity artificial endocranium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of the inner layer: the inner layer material is polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.), dissolved in hexafluoroisopropanol (Suzhou Haofan Biological Co., Ltd.), and the concentration of the spinning solution is 5% (wt), stirring for 1 hour, electrospinning parameters: flow rate 1.0ml / h, voltage 12kV, receiving distance 15cm, electrospinning 2h;

[0049] (2) Preparation of the middle layer.

[0050] Carboxymethyl chitosan (Nantong Lvshen Biological Engineering Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0051] Polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0052] The two kinds of thin strips are braided by the rhombus weaving m...

Embodiment 2

[0059] (1) Preparation of the inner layer: the inner layer material is polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.), which is dissolved in hexafluoroisopropanol (Suzhou Haofan Biological Co., Ltd.), and the concentration of the spinning solution is 7%wt, stirring for 4 hours, electrospinning parameters: flow rate 0.9 ml / h, voltage 13kV, receiving distance 20cm, electrospinning 4h;

[0060] (2) Preparation of the middle layer.

[0061] Carboxymethyl chitosan (Nantong Lvshen Biological Engineering Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0062] Polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0063] The two kinds of thin strips are braided by the rhombus w...

Embodiment 3

[0070] (1) Preparation of the inner layer: the inner layer material is polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.), which is dissolved in hexafluoroisopropanol (Suzhou Haofan Biological Co., Ltd.), and the concentration of the spinning solution is 5% wt, stirred for 4 hours, electrospinning parameters were flow rate 1.0ml / h, voltage 15kV, receiving distance 25cm, electrospinning 5h.

[0071] (2) Preparation of the middle layer.

[0072] Carboxymethyl chitosan (Nantong Lvshen Biological Engineering Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0073] Polycaprolactone (Shenzhen Boli Biomaterials Co., Ltd.) was prepared into a film by electrospinning, and the thickness of the film was 40 μm. The film was made into thin strips with a width of 3 mm and a length of 1000 mm.

[0074] The two kinds of thin strips are braided by the rhombu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a multilayer nanofiber artificial endocranium containing cell growth factors. The multilayer nanofiber artificial endocranium comprises three layers, i.e., a hydrophobic inner layer facing the brain, a hydrophilic outer layer backing the brain and a weaving type middle layer positioned between the hydrophobic inner layer and the hydrophilic outer layer, wherein the inner layer is a nonoriented fiber film; the outer layer is an oriented nanometer fiber film; the cell growth factors are uniformly attached into fiber gaps of the outer layer; the middle layer is woven by strips of hydrophilic materials and hydrophobic materials. The invention also provides a preparation method of the multilayer nanofiber artificial endocranium containing the cell growth factors. The method comprises the following steps of preparing the hydrophobic inner layer through electric spinning; putting the middle layer which is woven in advance onto the upper surface of the inner layer; preparing the outer layer by electric spinning; spraying the cell growth factors on the outer layer; finally performing disinfection to obtain the artificial endocranium. The artificial endocranium provided by the invention effectively solves the problems that the restoration period of the endocranium is long, and the utilization rate of the cell growth factors is low. The application prospects are wide.

Description

technical field [0001] The invention relates to a multilayer high-strength artificial dura mater containing cytokines and a preparation method thereof, belonging to the technical field of biological micro-nano manufacturing. Background technique [0002] Dural defects are common in neurosurgery clinical work. Open craniocerebral injuries (industry, traffic, war, etc.), tumor erosion, congenital meningeal defects and other craniocerebral diseases can cause dural defects. The dural defect needs to be repaired in time to prevent cerebrospinal fluid from overflowing, to prevent the swelling of the brain and the compression of atmospheric pressure, otherwise it will endanger human life. Dural defects can also cause complications such as intracranial infection, brain adhesions, and subcutaneous fluid, often causing diseases such as headaches and brain dysfunction. [0003] At present, the artificial dura mater made of various materials is being clinically used, which can be divid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/20A61L27/50A61L27/54D04H1/728D01D5/00
CPCA61L27/18A61L27/20A61L27/50A61L27/54A61L2300/252A61L2300/414A61L2430/32D01D5/003D01D5/0069D01D5/0076D01D5/0092D04H1/728C08L67/04C08L5/08
Inventor 李瑞锋王斐李典李翰林
Owner 李瑞锋
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com