Calcium-containing compound separated from vinegar egg liquid, and application thereof

A compound and composition technology, applied in the fields of medicine and food, can solve the problems of unclear functional components, unclear efficacy mechanism, no separation of calcium acetate compounds, etc., and achieve the effect of lowering blood pressure

Active Publication Date: 2018-02-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific functional ingredients are not clear, and the mechanism of efficacy cannot be clarified
Related literature reports that vinegar egg liquid contains a large amount of calcium and exists in the form of calcium acetate, but there is no report on the separation of calcium acetate compounds with the above effects from vinegar egg liquid

Method used

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  • Calcium-containing compound separated from vinegar egg liquid, and application thereof
  • Calcium-containing compound separated from vinegar egg liquid, and application thereof
  • Calcium-containing compound separated from vinegar egg liquid, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The extraction of embodiment 1 sugar calcium compound

[0024] (1) Preparation of Vinegar Egg Liquid: add 10kg eggs to 15kg 9° vinegar, soak for 24 hours, break the egg skin, soak for another 24 hours, filter to get the filtrate, and obtain Vinegar Egg Liquid.

[0025] (2) Concentrate 10L vinegar egg liquid to 2L volume, add 8L volume of 95% ethanol while stirring, let stand for more than 4h, filter to collect precipitate.

[0026] (3) Add 0.5 liters of water to dissolve the precipitate, filter to remove impurities, and separate the supernatant with an MCI column (5×150cm), first elute with 6 liters of deionized water, and then elute with 9 liters of 10% ethanol , the 10% ethanol eluate was collected and concentrated to a volume of 1.8 L.

[0027] (4) The 10% ethanol eluted concentrated solution is repeatedly separated by the ODS-AQ column, first eluted with deionized water, then eluted with 6% ethanol, and repeatedly separated by the ODS-AQ column for 2 to 3 times. T...

Embodiment 2

[0028] The identification of embodiment 2 sugar calcium compound

[0029] The compounds obtained in Example 1 were identified by HPLC\HPLC-MS and nuclear magnetic resonance methods, respectively.

[0030] (1)HPLC

[0031] Using ODSC18 chromatographic column, methanol: water = 6: 94 mobile phase, flow rate 0.3mL / min, detection wavelength 228nm chromatogram as shown figure 1 Shown; Retention time is that the chromatographic peak at 1.56min place is the compound that step 1 obtains.

[0032] (2)HPLC-MS

[0033] Using ESI+ mode, the voltage is 1800v, ​​the mass spectrum is as follows figure 2 shown.

[0034] (3) NMR, with D 2 O is solvent, tetramethylsilane is internal standard, Bruck 500M Z NMR measurements were carried out.

[0035] Table 1 NMR results

[0036]

[0037] From 1 H-NMR shows δ5.11 (1H, d, J=3.6), 4.51 (1H, d, J=7.6), these two Hs should be the end group signal of the sugar, 3.0--4.1 is the H signal on the sugar, 1.79(1H, s), should be a methyl signal....

Embodiment 3

[0039] The application of embodiment 3 sugar calcium compound in lowering blood pressure

[0040] In the present invention, the inhibitory effect on angiotensin-converting enzyme (ACE) is used to test the blood pressure-lowering effect of sugar and calcium. Prepare 100 μL of 50, 100, and 200 μg / mL sugar-calcium solutions, add 40 μL of 25 mU ACE enzyme solution respectively, incubate at 37 ° C for 30 minutes, add 200 μL of 1 mol / L hydrochloric acid, and measure the absorbance at a wavelength of 228 nm. Taking deionized water as the blank, the difference ratio between different concentrations of sugar calcium and the blank is the inhibition rate, and the results are shown in Table 2.

[0041] Table 2. Inhibitory effect of calcium sugar on ACE

[0042]

[0043] ACE is an angiotensin-converting enzyme, which acts through the renin-angiotensin system and the kallikrein-kinin system to increase blood pressure. Inhibiting the activity of ACE can lower blood pressure. It can be s...

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Abstract

The invention discloses a calcium-containing compound separated from a vinegar egg liquid, and an application thereof, and belongs to the technical fields of foods and medicines. The compound having blood pressure and blood sugar lowering functions is extracted from a vinegar soaked egg solution, and the blood pressure and blood sugar lowering effects of the compound are verified. A result shows that the IC50 value on ACE inhibition reaches 11.7-34 [mu]g / ml 30 min after 50-200 [mu]g / mL of the sugar calcium compound is added; and an animal experiment result shows that the fasting blood sugar ofdiabetic mice is decreased by 27.67% two weeks after the compound is administrated.

Description

technical field [0001] The invention relates to a calcium-containing compound separated from vinegar egg liquid and application thereof, belonging to the technical fields of food and medicine. Background technique [0002] Vinegar egg liquid is a traditional food therapy product in my country, which was recorded in "Treatise on Febrile Diseases" by Zhang Zhongjing in the late Eastern Han Dynasty. Vinegar egg liquid has the functions of softening blood vessels, lowering blood pressure, lowering cholesterol, and lowering blood sugar. It has a certain preventive effect on more than 70 diseases such as cardiovascular and cerebrovascular, liver and kidney. However, the specific functional ingredients are not clear, and the mechanism of efficacy cannot be clarified. Related literature reports that vinegar egg liquid contains a large amount of calcium and exists in the form of calcium acetate, but there is no report on the separation of calcium acetate compound with the above-men...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06A23L33/125A61P3/06A61P9/12A61P9/00A61P1/16A61P13/12
CPCA23L33/125A23V2002/00C07H3/06A23V2200/328A23V2200/3262A23V2200/326
Inventor 徐德平
Owner JIANGNAN UNIV
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