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Screening plasmid for antioxidant compound based on prawn glutathione reductase gene promoter, screening method and application

An anti-oxidation and compound technology, which is applied in the field of screening plasmids for anti-oxidation compounds, to achieve high accuracy and accuracy

Active Publication Date: 2018-02-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there is no report on the screening method of antioxidant compounds based on the promoter of glutathione reductase gene in shrimp

Method used

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  • Screening plasmid for antioxidant compound based on prawn glutathione reductase gene promoter, screening method and application
  • Screening plasmid for antioxidant compound based on prawn glutathione reductase gene promoter, screening method and application
  • Screening plasmid for antioxidant compound based on prawn glutathione reductase gene promoter, screening method and application

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Embodiment Construction

[0052] The following descriptions are preferred implementations of the embodiments of the present invention. It should be pointed out that those skilled in the art can make some improvements and modifications without departing from the principles of the embodiments of the present invention. These improvements And retouching are also regarded as the scope of protection of the embodiments of the present invention.

[0053] Unless otherwise specified, the reagents and consumables used in the examples of the present invention are commercially available.

[0054] Unless otherwise indicated, conventional techniques of immunology, biochemistry, chemistry, molecular biology, microbiology, cell biology, genomics and recombinant DNA, which are within the skill of the art, are employed in the examples of the present invention. Including but not limited to: Sambrook, Fritsch, and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL, vol. 2 Minor Editor (1989); CURRENT PROTOCOLS IN MOLECULAR ...

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Abstract

The invention provides a screening plasmid for an antioxidant compound based on a prawn glutathione reductase gene promoter, a screening method and an application. According to the technical scheme provided by the invention, a double luciferase reporter gene system is utilized to realize the semi-automatic large-scale high throughput screening for the candidate compounds. The invention also specifically provides the application of MC2 and / or MC3 in regulating LvGST activity, regulating and controlling NRF2-Keapl channel or preparing a drug for preventing and controlling the diseases caused byoxidative stress.

Description

technical field [0001] The invention relates to the fields of molecular biology and targeted drug screening, in particular to a screening plasmid, screening method and application of an antioxidant compound based on the promoter of the glutathione reductase gene of prawns. Background technique [0002] Substance and energy metabolism are constantly occurring in the body, and accompanied by various oxidation reactions, many free radical molecules are produced in the cells. Among them, reactive oxygen species (reactive oxygen species, ROS) is the most important and well-studied category, so it is often used as a characteristic molecule of oxidative stress. [0003] There are two sources of ROS: one is endogenous, the reactive oxygen species leakage during mitochondrial respiratory chain oxidative phosphorylation and peroxisomes and activated inflammatory cells; the other is exogenous, such as exogenous Induced by source compounds, pathogens, pro-inflammatory cytokines and hea...

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Application Information

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IPC IPC(8): C12N15/113C12N15/63C12N15/86C12Q1/66A61K31/133A61K31/16A61P39/06
CPCA61K31/133A61K31/16C12N9/0051C12Q1/66C12Y108/01007
Inventor 陈义烘翁少萍何建国
Owner SUN YAT SEN UNIV
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