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Preparation capable of improving sensibility of bacteria on antibiotics

An antibiotic and sensitivity technology, applied in the field of glycine and glucose, to achieve the effect of improving sensitivity

Inactive Publication Date: 2018-02-27
厦门显德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no reports of glycine-promoting antibiotics inhibiting the growth of resistant bacteria and glycine increasing glucose-promoting antibiotic effects

Method used

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  • Preparation capable of improving sensibility of bacteria on antibiotics
  • Preparation capable of improving sensibility of bacteria on antibiotics
  • Preparation capable of improving sensibility of bacteria on antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Determination of drug resistance of Edwardsiella tarda EIB202

[0034] Edwardsiella tarda is a Gram-negative short bacillus. It was first reported by Hoshina (1 96 2a) and is related to Japanese eel red disease (reddisease). From the first report to now, the fungus has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, carp, etc., causing huge losses to aquaculture. Edwardsiella tarda is also a common pathogenic bacteria in humans and fish, which directly poses a threat to human health.

[0035] Firstly, the minimum inhibitory concentration of Edwardsiella tarda to various antibiotics was determined. The results showed that the minimum inhibitory concentration of Edwardsiella tarda EIB202 to kanamycin was 12.5 μg / mL, the minimum inhibitory concentration to tetracycline was 125 μg / mL, and the minimum inhibitory concentration to chloramphenicol was 50 μg / mL, indicating that Edwardsiella tarda EIB202 is a multidru...

Embodiment 2

[0037] Glycine increases the susceptibility of Edwardsiella tarda to kanamycin

[0038] 1. Preparation of test samples: Pick a single colony of Edwardsiella tarda EIB202 from the LB plate and inoculate it in 5 mL of LB medium, culture at 200 rpm at 30°C for 24 hours to reach saturation. Collect the bacterial liquid by centrifugation, centrifuge at 8000rpm for 5min, remove the supernatant and wash the bacterial cells with 0.85% normal saline, and finally suspend the bacterial cells with 1×M9 (containing 10mM acetate), adjust the OD value of the bacterial solution to 0.2, and then pack them separately 5mL in a test tube for later use.

[0039] 2. Glycine can increase the sensitivity of Edwardsiella tarda EIB202 to kanamycin

[0040] In order to know whether glycine contributes to the bactericidal effect of bacteria, the experiment of adding glycine under the premise of adding kanamycin was carried out. To the above prepared bacterial samples, first add 40 μg / mL kanamycin, then...

Embodiment 3

[0042] Glucose synergizes with glycine to increase susceptibility of Edwardsiella tarda

[0043] It has been reported in the literature that glucose can increase the sensitivity of aminoglycosides to Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) persistent drug-resistant bacteria (persistence), and it has been proved that this phenomenon is the Unique to glycoside antibiotics. Glucose, as well as glycine and glucose, were therefore also studied for the susceptibility of bacteria to kanamycin.

[0044] 1. Glucose and glycine can synergistically increase the susceptibility of Edwardsiella tarda to kanamycin

[0045]In order to study whether glucose and glucose can cooperate with glycine to increase the sensitivity of bacteria to kanamycin, the experiment was divided into three groups: one group only added 40 μg / mL kanamycin in the prepared samples as a control, and the other two groups On the basis of adding 40 μg / mL kanamycin, 10 mM glucose, 10 mM...

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Abstract

The invention discloses a preparation capable of improving sensibility of bacteria on antibiotics and relates to glycine and glucose. Because glycine serving as small molecules capable of improving the sensibility of bacteria on antibiotics achieves an effect of inhibiting drug-resistance bacteria, glycine can be applied to improve the sensibility of bacteria on antibiotics. Because glycine and glucose serving as small molecules capable of improving the sensibility of bacteria on antibiotics achieve an effect of inhibiting drug-resistance bacteria, glycine and glucose can be combined to be applied to improve the sensibility of bacteria on antibiotics. The preparation is composed of glycine, glucose and antibiotics in a mass ratio of 1:(0.01-100):(0.01-10). A usage amount of glycine is 0.1mg-1g / kg per time. After glycine is added, the survival rate of slow Edwardsiella drug-resistance bacteria treated by kanamycin is obviously decreased, meaning the sensibility of slow Edwardsiella drug-resistance bacteria to kanamycin can be improved.

Description

technical field [0001] The present invention relates to glycine and glucose, and in particular to a preparation capable of increasing the susceptibility of bacteria to antibiotics. Background technique [0002] Although the use of antibiotics plays an essential role in the protection of human health and life and intensive animal farming, due to the abuse and misuse of antibiotics, it has become a threat to human health, livestock and poultry farming, aquaculture and the ecological environment. The key factor. Therefore, the control of bacterial antibiotic resistance is very important. [0003] At present, antibiotics are widely used in animal husbandry. On the one hand, some antibiotics are essential as veterinary drugs to control bacterial infections; on the other hand, some antibiotics can promote animal growth as feed drug additives. The extensive use of antibiotics has caused a large number of sensitive bacteria to die, and drug-resistant bacteria to multiply, which h...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K31/198A61K31/7004A61K31/7036A61P31/04
CPCA61K31/198A61K31/7004A61K31/7036A61K45/06A61K2300/00
Inventor 谢荔朋
Owner 厦门显德生物科技有限公司
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