Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Finger-print spectrum detection method for mint essential oil

A technology of peppermint essential oil and fingerprint, which is applied in the field of detection and analysis, can solve the problems of poor qualitative results and long detection time, and achieve the effects of convenient identification, reduced detection cost, and reduced detection time

Active Publication Date: 2018-03-02
安徽出入境检验检疫局检验检疫技术中心 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve technical problems such as long detection time and poor qualitative effect in the prior art, the present invention provides a fast and effective fingerprint detection method of peppermint essential oil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Finger-print spectrum detection method for mint essential oil
  • Finger-print spectrum detection method for mint essential oil
  • Finger-print spectrum detection method for mint essential oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Establish standard control fingerprints of peppermint essential oil:

[0035] Take 100 μL of peppermint essential oil, add n-hexane to 1 mL to obtain a dilution of peppermint essential oil, pass it through a 0.22 µm organic filter membrane, enter into a convergence chromatograph for detection, and measure with a PDA detector.

[0036] Convergence chromatography conditions: using Acqtity UPC 2 BEH chromatographic column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure of 2000 psi, column temperature of 40 °C, column flow rate of 0.8 mL / min, mobile phase A and mobile phase B, mobile phase A is supercritical CO 2 , the mobile phase B is acetonitrile-methanol solution (the volume ratio of acetonitrile-methanol is 1:1); the initial mobile phase is 100% mobile phase A, gradient elution, the PDA wavelength acquisition range is 200 nm ~ 310 nm, the injection volume Preferably 1.0 μL.

[0037] Gradient elution conditions: initial mobile phase hold for 2 min; 2 ~ 3 ...

Embodiment 2

[0041] Sample: Peppermint Essential Oil

[0042] 1. Take 100 μL of peppermint essential oil, add n-hexane to 1 mL, pass through a 0.22 µm organic filter membrane for sample injection, and obtain the dilution of peppermint essential oil to be tested;

[0043] 2. Carry out the convergence chromatographic detection of the peppermint essential oil dilution to be tested, and measure in conjunction with the PDA detector, obtain the convergence chromatographic fingerprint of the peppermint essential oil, see figure 2 ;

[0044] Convergence chromatography conditions: using Acqtity UPC 2 BEH chromatographic column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure of 2000 psi, column temperature of 40 °C, column flow rate of 0.8 mL / min, mobile phase A and mobile phase B, mobile phase A is supercritical CO 2 , the mobile phase B is acetonitrile-methanol solution (the volume ratio of acetonitrile-methanol is 1:1); the initial mobile phase is 100% mobile phase A, gradient eluti...

Embodiment 3

[0048] Sample: Spearmint Oil

[0049] 1. Take 100 μL of spearmint oil, add n-hexane to 1 mL, pass through a 0.22 µm organic filter membrane for sample injection, and obtain the dilution of spearmint oil to be tested;

[0050] 2. Carry out the convergence chromatographic detection of the spearmint oil dilution to be measured, and measure in conjunction with the PDA detector, obtain the convergence chromatographic fingerprint of the spearmint oil, see image 3 ;

[0051] Convergence chromatography conditions: using Acqtity UPC 2 BEH chromatographic column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure of 2000 psi, column temperature of 40 °C, column flow rate of 0.8 mL / min, mobile phase A and mobile phase B, mobile phase A is supercritical CO 2 , the mobile phase B is acetonitrile-methanol solution (the volume ratio of acetonitrile-methanol is 1:1); the initial mobile phase is 100% mobile phase A, gradient elution, the PDA wavelength acquisition range is 200 nm ~...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a finger-print spectrum detection method for mint essential oil. The mint essential oil takes hexane as a dilution solvent, a convergence chromatographic instrument is used fordetection, a PDA detector is combined for determination, a characteristic peak PDA spectrogram library is established, and a mint essential oil standard contrast finger-print spectrum is established.The chromatographic peak PDA spectrogram library in the convergence chromatographic instrument of the to-be-detected mint essential oil is extracted and is compared with a characteristic peak spectrum spectrogram library through retrieval, and a common characteristic peak is indentified; an included angle cosine method is used for evaluating similarity of the characteristic peak and the standardcontrast finger-print spectrum of the mint essential oil, when the similarity is greater than or equal to 0.90, the to-be-detected mint essential oil is determined as pure mint essential oil, and whenthe similarity is less than 0.90, the to-be-detected mint essential oil is determined as non pure mint essential oil; rapid and effective discriminating of the mint essential oil is realized, and themethod has certain meaning for quality control and adulteration identification of the mint essential oil.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a peppermint essential oil fingerprint detection technology. Background technique [0002] Mint is a labiatae plant and an important natural spice plant. Peppermint essential oil is the volatile oil extracted from the fresh stems and leaves of peppermint through steam distillation, freezing, and partial debrain processing. It is a colorless or light yellow clear liquid with a special cool aroma. Peppermint essential oil contains alcohols, ketones, esters, terpenes and other active components, and has the effects of dispelling wind and clearing heat. Peppermint essential oil and its derivatives have antibacterial activity and antioxidant properties, and are widely used in various cosmetics, food, medicine, tobacco and other fields. Peppermint essential oil is one of the most widely used and used natural spices. With the development of the world economy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/74G01N30/06
Inventor 宋伟韩芳吕亚宁胡艳云周典兵丁磊郑平邓晓军盛旋
Owner 安徽出入境检验检疫局检验检疫技术中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com