A fingerprint detection method of ginger essential oil

A fingerprint spectrum and detection method technology, applied in the field of detection and analysis, can solve problems such as long detection time and poor qualitative effect, and achieve the effects of reducing detection cost, facilitating identification, and avoiding thermal retake and thermal conversion effects.

Active Publication Date: 2019-10-15
安徽出入境检验检疫局检验检疫技术中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve technical problems such as long detection time and poor qualitative effect in the prior art, the present invention provides a fast and effective fingerprint detection method of ginger essential oil

Method used

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  • A fingerprint detection method of ginger essential oil
  • A fingerprint detection method of ginger essential oil
  • A fingerprint detection method of ginger essential oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Establish standard reference fingerprint of ginger essential oil

[0036] Take 100 μL of ginger essential oil, add n-hexane to 1 mL to obtain the pure ginger essential oil dilution, pass through a 0.22 μm organic filter membrane and enter it into a convergent chromatograph for detection by a PDA detector (wavelength collection range: 200-310 nm).

[0037] Convergence chromatographic conditions: Acqtity UPC 2 BEH column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure is 2000 psi, column temperature is 40 ℃, column flow rate is 0.8 mL / min, mobile phase is supercritical CO 2 (Mobile phase A) and acetonitrile-methanol solution (mobile phase B) with a volume ratio of 1:1, initial mobile phase is 100% A, gradient elution, collection wavelength range 200 nm ~ 310 nm, injection volume is preferably 4.0 μL.

[0038] Gradient elution conditions: initial mobile phase hold for 2 min; 2 ~ 3 min, 100% A ~ 99.8% A, hold for 2 minutes; 5 ~ 6 min, 99.8% A ~ 99.5% A, hold for 2 min; ...

Embodiment 2

[0042] Sample: ginger essential oil

[0043] 1. Take 100 μL of ginger essential oil to be tested, add n-hexane to 1 mL to obtain a dilution of ginger essential oil to be tested, and pass through a 0.22μm organic filter membrane for injection;

[0044] 2. The ginger essential oil dilution to be tested is tested by a convergence chromatograph, combined with the PDA detector to determine the convergence chromatographic fingerprint of the ginger essential oil to be tested, see figure 2 .

[0045] Convergence chromatographic conditions: Acqtity UPC 2 BEH column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure is 2000 psi, column temperature is 40 ℃, column flow rate is 0.8 mL / min, mobile phase is supercritical CO 2 (Mobile phase A) and acetonitrile-methanol solution (mobile phase B) with a volume ratio of 1:1, initial mobile phase is 100% A, gradient elution, collection wavelength range 200 nm ~ 310 nm, injection volume is preferably 4.0 μL.

[0046] Gradient elution conditions:...

Embodiment 3

[0049] Sample: soybean oil

[0050] 1. Take 100 μL of soybean oil, add n-hexane to 1 mL to obtain the soybean oil dilution to be tested, and pass the 0.22 μm organic filter membrane to be injected;

[0051] 2. The soybean oil diluent to be tested is tested by a convergence chromatograph, combined with a PDA detector to obtain the convergence chromatographic fingerprint of soybean oil, see image 3 .

[0052] Convergence chromatographic conditions: Acqtity UPC 2 BEH column (50 mm×2.1 mm, 1.8 μm), PDA detector, system back pressure is 2000 psi, column temperature is 40 ℃, column flow rate is 0.8 mL / min, mobile phase is supercritical CO 2 (Mobile phase A) and acetonitrile-methanol solution (mobile phase B) with a volume ratio of 1:1, initial mobile phase is 100% A, gradient elution, collection wavelength range 200 nm ~ 310 nm, injection volume is preferably 4.0 μL.

[0053] Gradient elution conditions: initial mobile phase hold for 2 min; 2 ~ 3 min, 100% A ~ 99.8% A, hold for 2 minutes;...

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Abstract

The invention discloses a fingerprint spectrum detection technology of ginger essential oil. In the detection of the ginger essential oil, n-hexane is taken as a diluting solvent, a coupling chromatograph is adopted for detection, a PDA (photo-diode array) detector is adopted for measurement, a PDA spectrogram database of a characteristic peak is established, and a standard control fingerprint spectrum of the ginger essential oil is established. The fingerprint spectrum detection technology comprises the following steps: extracting a PDA spectrogram of a chromatographic peak and the spectrogram database of the characteristic peak in a coupling chromatograph fingerprint spectrum of to-be-detected ginger essential oil for search and comparison, and identifying a common characteristic peak; estimating similarity to the standard control fingerprint spectrum of the ginger essential oil with an included angle cosine method; when the similarity is more than or equal to 0.90, determining thatthe to-be-detected ginger essential oil is pure ginger essential oil; and when the similarity is less than 0.90, determining that the to-be-detected ginger essential oil is not pure ginger essential oil. According to the detection technology, the ginger essential oil can be rapidly and effectively identified, and therefore, the detection technology has certain significance for quality control of peppermint essential oil and adulteration identification.

Description

Technical field [0001] The invention belongs to the technical field of detection and analysis, and specifically relates to a fingerprint detection technology of ginger essential oil. Background technique [0002] Ginger belongs to the ginger family, a perennial herb. The main producing areas are China, India, Sri Lanka, the United States and Europe. It is an important natural spice plant. Ginger essential oil is the volatile oil obtained by drying, grinding and steam distillation of ginger tubers. It is a pale yellow liquid with a pungent smell of ginger. The main components are gingerene, gingerol, gingerol, ginger ketone, citral, and cress Ene etc. Ginger essential oil is mainly used in the preparation of food flavors, various alcoholic beverages, soft drinks and candies, as well as perfumes and other cosmetics. In the field of personal care products, ginger essential oil has been widely used as a single or compound essential oil. It has the effects of dissipating blood conge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/74
Inventor 宋伟胡艳云韩芳吕亚宁周典兵丁磊郑平邓晓军盛旋
Owner 安徽出入境检验检疫局检验检疫技术中心
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