scalp hair conditioner
A kind of maintenance agent and scalp technology, applied in the field of nursing, can solve the problems of self-image damage, deterioration of hair quality, difficulty in improving hair loss, etc., and achieve the effect of not easy to bifurcate, relieve hair follicle inflammation, and induce hair follicle keratinocyte proliferation
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Embodiment 1
[0042] Comparison of Fullerenol Nano-Single Crystal Particles and Monodisperse Fullerenol
[0043] 1.1. Preparation of fullererol nano single crystal particles: 500mg C60 and 30mL H 2 o 2 React with NaOH or KOH (wt12%) at 80°C for 8 hours, wash and centrifuge to collect the precipitate, then add ultrapure water to dissolve it, and freeze-dry the dialyzed sample to obtain a fullerenol solid powder, which is sample 001.
[0044] 1.2. Monodisperse fullerenol (literature method): 500mg C60 was dissolved in 500mL toluene, 50 drops of 40% TBAH was added to 20Ml 10mol / L NaOH as a phase transfer catalyst, then the toluene solution of C60 was added to it, and then slowly added dropwise 5mL of 30% H2O2 solution, the C60 toluene solution changed from purple to colorless after a period of reaction, while the aqueous solution changed from colorless to dark brown. After continuing the reaction for a period of time, separate the organic phase from the water phase with a separatory funnel, a...
Embodiment 2
[0055] Fulleric acid nano single crystal particles
[0056] 2.1 Preparation of fullerene nano single crystal particles: Bingle-Hirsch reaction to prepare fullerene ester derivatives, and then combine fullerene ester derivatives with H 2 o 2 React with NaOH or KOH (wt 12%) mixed solution at 80°C for 8h, wash and centrifuge to collect the precipitate, then add ultrapure water to dissolve it, freeze-dry the dialyzed sample to obtain fulvic acid solid powder. Sample 003
[0057] 2.2. ESR DMPO-OH spin trapping method to test the free radical scavenging ability of sample 003: irradiate hydrogen peroxide with ultraviolet light for 4 minutes to induce the generation of hydroxyl radicals, then add the sample to be tested into the system, and observe the changes in the test signal of hydroxyl radicals. Such as Figure 5 As shown, sample 003 has a good scavenging effect on hydroxyl radicals
[0058] 2.3. Cell protection (hydrogen peroxide damage): firstly, different concentrations of...
Embodiment 3
[0066] 3.3 Preparation of fullerene ethylenediamine nano single crystal particles: fullerene C60 and ethylenediamine are heated and reacted. After the reaction, the unreacted ethylenediamine is removed under reduced pressure to obtain a brown product, which is dissolved in water and recrystallized with acetone , precipitate flocculent precipitate, collect the precipitate, vacuum dry to obtain fullerene ethylenediamine solid, dialyze and then dry to obtain sample 004.
[0067] 3.2. ESR DMPO-OH spin trapping method to test the free radical scavenging ability of sample 004: irradiate hydrogen peroxide with ultraviolet light for 4 minutes to induce the generation of hydroxyl radicals, then add the sample to be tested into the system, and observe the changes in the test signal of hydroxyl radicals, such as Figure 9 As shown, sample 004 has a better scavenging effect on hydroxyl radicals than the blank.
[0068] 3.3. Cell protection (hydrogen peroxide damage): firstly, different co...
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