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Determination method of ganoderma acid a component in ganoderma syrup

A determination method and technology of ganoderma acid, applied in the field of content determination of Chinese patent medicines, can solve the problems of high column temperature, large chromatographic column damage, and poor resolution of ganoderma A, so as to reduce detection costs, improve product quality, and improve detection. effect of effect

Active Publication Date: 2020-09-25
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] When this method actually detects the content of ganoderma acid A in ganoderma syrup, the resolution of ganoderma acid A is poor.
And the column temperature is too high, which will cause greater damage to the chromatographic column

Method used

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  • Determination method of ganoderma acid a component in ganoderma syrup
  • Determination method of ganoderma acid a component in ganoderma syrup
  • Determination method of ganoderma acid a component in ganoderma syrup

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 detects the content of active ingredient ganoderma acid A in ganoderma syrup according to the following method:

[0048] (1) Preparation of the test solution: Accurately measure 20 mL of ganoderma lucidum syrup, extract with 50 mL of ethyl acetate, repeat the extraction 3 times, combine the upper layer and evaporate to dryness, dissolve the residue with methanol, and set the volume in a 5 mL volumetric flask. Shake well, filter with 0.45μm microporous membrane, as the test solution;

[0049] (2) Preparation of reference substance solution: take ganoderma acid A reference substance, add methanol and dissolve to make a 133 μg / mL solution, to obtain final product;

[0050] (3) Determination method: respectively accurately draw 10 μ L of the test solution and the reference solution, inject into the liquid chromatograph, measure and calculate;

[0051] The chromatographic condition of described determination method is: InertSustain C18 chromatographic column, m...

Embodiment 2

[0053] Embodiment 2 detects the content of active ingredient ganoderma acid A in ganoderma syrup according to the following method:

[0054] (1) Preparation of the test solution: Precisely measure 10 mL of ganoderma lucidum syrup, extract with 30 mL of ethyl acetate, repeat the extraction twice, combine the upper liquid and evaporate to dryness, dissolve the residue with methanol, and dilute it in a 10 mL volumetric flask. Shake well, filter with 0.45μm microporous membrane, as the test solution;

[0055] (2) Preparation of reference substance solution: take ganoderma acid A reference substance, add methanol to dissolve to make a 100 μg / mL solution, and obtain it;

[0056] (3) Measuring method: each 5 μ L of need testing solution and reference substance solution are drawn accurately respectively, inject liquid chromatograph, measure, calculate;

[0057] The chromatographic conditions of the assay method are the same as in Example 1.

[0058] Detect according to the method of...

Embodiment 3

[0059] Embodiment 3 detects the content of active ingredient ganoderma acid A in ganoderma syrup according to the following method:

[0060] (1) Preparation of the test solution: accurately measure 30 mL of Ganoderma lucidum syrup, extract with 60 mL of ethyl acetate, repeat the extraction 4 times, combine the upper layer and evaporate to dryness, dissolve the residue with methanol, and dilute it in a 5 mL volumetric flask. Shake well, filter with 0.45μm microporous membrane, as the test solution;

[0061] (2) Preparation of reference substance solution: take ganoderma acid A reference substance, add methanol to dissolve to make a 200 μg / mL solution, and obtain it;

[0062] (3) Measuring method: each 8 μ L of need testing solution and reference substance solution are drawn accurately respectively, inject liquid chromatograph, measure, calculate;

[0063] The chromatographic conditions of the assay method are the same as in Example 1.

[0064] Detect according to the method o...

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Abstract

The invention relates to a method for determining the content of a ganoderenic acid A component in Ganoderma lucidum syrup. The method comprises the following steps: preparing a sample solution, preparing a reference substance solution, and carrying out high performance liquid chromatography determination. The content determination method has the advantages of stable detection base line, high resolution, high detection accuracy and good durability during determination, and also has the advantages of shortening of the content determination time, stable improvement of the detection efficiency, reduction of the dosage of toxic chemical reagents, realization of economic and environmentally-friendly properties; and the method for determining the content of the ganoderenic acid A in the Ganoderma lucidum syrup through high performance liquid chromatography is established for the first time, and provides an effective quality detection method for well improving the product quality of the Ganoderma lucidum syrup.

Description

technical field [0001] The invention relates to content determination of Chinese patent medicines, more specifically, a method for content determination of active ingredient ganoderma acid A in ganoderma syrup. Background technique [0002] Ganoderma lucidum syrup is a syrup made from Ganoderma lucidum. It has the functions of nourishing the heart and calming the nerves, invigorating the spleen and stomach. It is used for the auxiliary treatment of palpitations, insomnia, loss of appetite, neurasthenia, hyperlipidemia, coronary heart disease, and chronic bronchitis. Ganoderma acid is one of the main active ingredients in Ganoderma lucidum, which can regulate the body's glucose metabolism, protect the liver, resist oxidation, and improve immunity. [0003] The original standard of Ganoderma lucidum syrup was recorded on page 122 of the seventeenth volume of "Chengfang Preparations". The standard did not disclose the content determination method of its active ingredients, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 付卡利刘逆夫龚云
Owner ZHUZHOU QIANJIN PHARMA
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