Method for extracting isoquercitrin from herba desmodii styracifolii

A technology for Radix vulgaris and isoquercitrin, which is applied in the extraction field in the field of biotechnology and can solve the problems of small relative molecular mass, complicated operation, low solubility and the like

Inactive Publication Date: 2018-03-27
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the common solvent CO 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entrainer in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so supercritical CO 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting isoquercitrin from herba desmodii styracifolii
  • Method for extracting isoquercitrin from herba desmodii styracifolii

Examples

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Embodiment 1

[0024] A method for extracting isoquercitrin from Desmodium glabra, specifically comprising the steps of:

[0025] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross a 20-mesh sieve;

[0026] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0027] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, and load the...

Embodiment 2

[0029] A method for extracting isoquercitrin from Desmodium glabra, specifically comprising the steps of:

[0030] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and pass through a 40-mesh sieve;

[0031] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0032]3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous resin, ...

Embodiment 3

[0034] A method for extracting isoquercitrin from Desmodium glabra, specifically comprising the steps of:

[0035] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross an 80-mesh sieve;

[0036] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0037] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, and load the...

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Abstract

The invention discloses a method for extracting isoquercitrin from Desmodium sativa, comprising the following steps: 1) Cleaning the grass of Desmodium, drying it, pulverizing it with a pulverizer, and sieving it; 2) Subcritical water extraction: put the processed raw materials in the extraction kettle, add 0.001 parts by weight of hydroxyethyl cellulose equivalent to the raw materials, inject deoxygenated deionized water into the kettle, the extraction temperature is 185-200°C, and the pressure is 8-10Mpa , the extraction time is 0.5-1.0h, extracting Desmodium sativa, the extraction is finished, the pressure is released, and the extract flows into the collection tank and is cooled to room temperature to obtain the extract; 3) Purification: the extract obtained in step 2) is concentrated, passed The macroporous resin is subjected to column chromatography, eluted with 50% v/v ethanol to obtain an eluent, concentrated and dried to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting isoquercitrin from Desmodium sativa. Background technique [0002] Isoquercitrin, alias: quercetin-3-O-glucoside, isoquercetin, apocynum. Molecular formula: C 21 h 20 o 12 , molecular weight: 464.38. Yellow needle-like crystals (water), melting point 225-227°C, almost insoluble in cold water, slightly soluble in boiling water, soluble in alkali solution to appear dark yellow. Yellow crystal (ethanol), melting point 206-208°C and 232-236°C. Pale yellow needle crystal, melting point 220-222°C (decomposition). Crystallization (hot water), melting point 230°C. Animal experiments have hypotensive effect. Tests such as capillary permeability have shown anti-inflammatory effects. It is toxic to the larvae of the cotton bollworm. It has enzyme-lowering effect and is one of the active ingredients of Tian Jihuang in treating hepatitis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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