Haematococcus pluvialis high-efficiency mariculture method

A technology of haematococcus pluvialis and mariculture, which is applied in the field of high-efficiency mariculture of haematococcus pluvialls, can solve the problem of low astaxanthin unit output, increase the accumulation of astaxanthin in single cells, and improve the reproductive capacity , The effect of promoting the accumulation of astaxanthin

Active Publication Date: 2018-03-27
HAINAN SANYUANXING BIOTECHNOLOGY CO LTD
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Problems solved by technology

Although the "one-step method" has the advantages of operation and pollution prevention, there is a problem that the unit yield of astaxanthin in Haematococcus pluvialis is low in the actual commercial production. Some studies have shown that the astaxanthin yield is only Half of the "two-step method", therefore, the current field still uses the "two-step method" as the main breeding method

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:

[0027] S1: Media Configuration

[0028] The medium comprises the following components: glucose 3g / L, KNO 3 0.6g / L, NaH 2 PO 4 0.1g / L, MgSO 4 ·7H 2 O0.1g / L, Fe-EDTA1mg / L, citric acid 0.5mg / L, pumpkin seed powder 0.3g / L, sweet potato powder 0.2g / L, seawater 50mL / L, the pH value of this medium is 5.0;

[0029] S2: Growth stage of Haematococcus pluvialis

[0030] The algal species were inoculated into the culture medium with an inoculation amount of 17%, and irradiated with a mixed light source formed by a combination of red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity was 2500Lx, the photoperiod was 12:12, the ambient temperature was controlled at 8°C, and carbon dioxide was introduced at a rate of 0.5 L / min per liter of medium, and the culture was cultivated for 2 days with continu...

Embodiment 2

[0034] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:

[0035] S1: Media Configuration

[0036] The medium comprises the following components: glucose 5g / L, KNO 3 1g / L, NaH 2 PO 4 0.3g / L, MgSO 4 ·7H 2 O0.2g / L, Fe-EDTA2mg / L, citric acid 1.0mg / L, pumpkin seed powder 0.7g / L, sweet potato powder 0.4g / L, seawater 100mL / L, the pH value of this medium is 6.0;

[0037] S2: Growth stage of Haematococcus pluvialis

[0038] The algal species were inoculated into the culture medium with an inoculation amount of 20%, and irradiated with a mixed light source formed by combining red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity is 3000Lx, the photoperiod is 12:12, the ambient temperature is controlled at 15°C, and carbon dioxide is introduced at a rate of 0.7L / min per liter of medium, and the culture is cultivated for 3 days with constant stirring at...

Embodiment 3

[0042] A high-efficiency seawater culture method for Haematococcus pluvialis, comprising the following steps:

[0043] S1: Media Configuration

[0044] The medium comprises the following components: glucose 4g / L, KNO 3 0.7g / L, NaH 2 PO 4 0.2g / L, MgSO 4 ·7H 2 0.2g / L, Fe-EDTA2mg / L, citric acid 0.7mg / L, pumpkin seed powder 0.5g / L, sweet potato powder 0.3g / L, seawater 70mL / L, the pH value of this medium is 5.5;

[0045] S2: Growth stage of Haematococcus pluvialis

[0046] The algal species were inoculated into the culture medium with an inoculation amount of 20%, and irradiated with a mixed light source formed by combining red light, green light and blue light (the intensity ratio of red light, green light and blue light was 3:1:2). The light intensity is 3000Lx, the photoperiod is 12:12, the ambient temperature is controlled at 10°C, and carbon dioxide is introduced at a rate of 0.6L / min per liter of culture medium. Stir continuously at 170rpm and cultivate for 2 days. , the...

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Abstract

The invention provides a haematococcus pluvialis high-efficiency mariculture method. On the basis of a traditional two-step method, culture conditions are adjusted reasonably, seawater is added for supplementing natural nutritional ingredients for algae, and rich seawater resources in Hainan and other coastal areas are fully utilized; a pumpkin seed powder, a sweet potato powder and a brainea insignis ethanol extract are added in a culture medium, sufficient nutritional ingredients are supplemented, and a role in preventing biological pollution is played; at the same time, through alternativeutilization of mixed light sources, algal propagation and astaxanthin accumulation can be effectively promoted. With adopting of the method, the cell density is as high as 10.56*10<5>/ml, the dry weight of cells is as high as 3.15 mg/ml, the astaxanthin content is as high as 104.12 mg/g, and the single-cell astaxanthin content is as high as 3.51*10<-2> [mu]g per cell. The method not only can effectively improve the reproductive capacity of haematococcus pluvialis, and moreover, the accumulation of the single-cell astaxanthin is significantly increased.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a high-efficiency seawater culture method for Haematococcus pluvialis. Background technique [0002] Natural astaxanthin is a highly efficient bioactive substance with strong antioxidant properties and broad application prospects. Compared with other organisms, Haematococcus pluvialis is recognized as the best biological source of natural astaxanthin. [0003] The latest research results show that under excellent culture conditions, the content of astaxanthin in Haematococcus pluvialis can reach up to 4-5%. At present, the methods for large-scale cultivation of Haematococcus pluvialis mainly include "two-step method" and "one-step method". The "two-step method" means: the first step is the vegetative growth stage, that is, to cultivate Haematococcus to obtain high-density growth of "green" cells. At this stage, Haematococcus pluvialis grows vigorously, but the content of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 郭红星周尽学
Owner HAINAN SANYUANXING BIOTECHNOLOGY CO LTD
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