Cardiac muscle ring culture method

Abstract

The invention belongs to the technical field of animal cell culture, and relates to a cardiac muscle ring culture method, which comprises: pre-treating a heart sample, and preparing small ventricularmuscle tissue blocks; digesting the small ventricular muscle tissue blocks, and terminating; collecting the supernatant after the terminating, and culturing to prepare an initial-stage cardiac musclering cell liquid; and carrying out cell liquid exchange on the initial-stage cardiac muscle ring cell liquid, and observing the dynamic growth of the cardiac muscle ring. According to the present invention, the cardiac muscle ring-like structure is found during the in vitro isolated culture of the cardiac muscle cells, and is initially named cardiac muscle ring, wherein the detailed research on the cell components, the forming mechanism and the differentiation potential of the cardiac muscle ring is necessary; and the representation of the structure can provide important significance for the further research on the cardiac muscle stem cell function and the molecular regulation mechanism thereof, the seed cell source enriching, the new approach for the research and development of cardiac muscle regeneration, and the like, can provide theoretical basis for the self-regeneration and differentiation regulation of cardiac stem cells, and can provide new ideas for the clinical treatment of ischemic heart disease.

Description

technical field [0001] The invention belongs to the technical field of animal cell culture, and relates to a method for culturing myocardial rings. Background technique [0002] Conventional wisdom holds that the heart is a terminally differentiated organ that cannot undergo endogenous repair. In recent years, c-kit isolated from the heart + and sca-1 + Cells proliferate rapidly and can differentiate into cardiac muscle, smooth muscle and endothelial cells, called cardiac stem cells. These stem cells can participate in the maintenance of myocardial homeostasis and pathological repair. According to various factors such as the expression of cell markers, it can be divided into purified cardiac stem cells and mixed cardiac stem cells. Purified cardiac stem cells have c-kit + cells, sca-1 + cells, CD117 + Cells, Isl + Cells, side population cells or SP cells, etc. Hybrid cardiac stem cells include cardiospheres (cardiosphere, CSP) and cardiosphere derived stem cells (ca...

AI Technical Summary

Problems solved by technology

Since the discovery of cardiac stem cells in 2003, many scholars have also begun to pay attention to the cardiac stem cell niche. Cardiac stem cells are clustered in the niche and can express c-kit, cardiac transcription factor Nkx2. Protein (α-sarcomericactin, α-SCA), smooth muscle cells, fibroblasts and endothelial cells are distributed in the niche, but its distribution in the myocardium, cell components in the niche, changes with age and disease, and regulation of the heart Molecular mechanisms underlying stem cell stabilization and differentiation are still unclear

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