Cardiac muscle ring culture method
A culture method and myocardial technology, which can be used in tissue culture, cell dissociation methods, biochemical equipment and methods, etc., can solve problems such as unclearness, and achieve the effect of enriching the source of seed cells
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[0022] 1. Materials and methods
[0023] 1. Experimental animals
[0024] A total of 80 SD rats born 1-3 days old, male or female, with an average body weight of 7.3±0.62g, were provided by the Experimental Animal Center of Xinxiang Medical College.
[0025] Isolation, Culture and Identification of Myocardial Ring Cells
[0026] 8 SD rats born 1-3 days old were soaked in 75% alcohol for 5 seconds, and the chest was opened under aseptic conditions in the ultra-clean workbench to take the heart, put it in 4°C sterile PBS solution, rinse 3-4 times, and wash away the remaining blood , Remove the large blood vessels connected to the heart, the atrial appendage and other tissues, and cut the ventricular muscle tissue into about 1mm with ophthalmic scissors 3 small pieces. Place in a 50mL sterile centrifuge tube, add 2mL each of 0.25% trypsin (Sigma) and 0.1% type Ⅰ collagenase (Sigma), pipette mechanically for 1min, put it in a 37°C constant temperature water bath for digestion f...
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