Method for detecting active ingredients in fructus evodiae juice
A technology of active ingredients and detection methods, applied in the field of detection of active ingredients in Evodia Fructus Juice, can solve the problems such as the absence of simultaneous detection of evodial alkaloids and evodial alkaloids, and achieves saving manpower and material costs, high precision, and stability good effect
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Embodiment 1
[0023] Preparation of the test solution: decoct Evodia rutaecarpa with water to obtain a water decoction of Evodia rutaecarpa, wherein the amount of water added is 12 times the amount of water, the soaking time is 30 minutes, decocted 3 times, each decocting 30 minutes; then the prepared Evodia rutaecarpa water Use a rotary evaporator to recover the solvent from the decoction, add 100% methanol to dissolve, ultrasonically extract for 30 minutes, let it cool, add 100% methanol to make up the weight, filter, centrifuge the filtrate at 16000r / min for 10min, take the supernatant and filter it with a 0.45μm microporous filter Membrane filtration, prepared the test solution.
[0024] Preparation of reference substance solution: Accurately weigh 2 mg (0.00244 g) of evodiamine reference substance, 1 mg (0.00127 g) of evodiamine reference substance, and 2 mg (0.00255 g) of evodiamine reference substance respectively, and add 100% methanol solution to volume to 25ml, 16000r / min high-spe...
Embodiment 2
[0030] Embodiment 2 linear range investigation
[0031]Draw 1.0 μl, 2.0 μl, 3.0 μl, 5.0 μl, 10.0 μl, and 20.0 μl of evodiamine, evodiamine, and evodiamine reference substance solutions respectively, inject them into the high-performance liquid chromatography, use Kromasil C18 column chromatography, and acetonitrile ( A)-0.01% phosphoric acid water (B) (51:49) was used as the mobile phase, the flow rate was 1.0ml / min, the injection volume was 10μl, the column temperature was 25°C, and the detection wavelength was 215nm. Draw the standard curve with the mass concentration of the reference substance as the abscissa (X) and the peak area as the ordinate (Y) to obtain the regression equation and correlation coefficient. The inspection results are shown in Table 1.
[0032] Table 1 Linear relationship inspection results
[0033]
[0034]
[0035] Take the mass concentration of evodiamine reference substance (mg / ml) as the abscissa [X], and take the peak area as the ordinate ...
Embodiment 3
[0037] With reference to the detection method of Example 1, the influence of the extraction solvent on the detection results was investigated. Take 10ml of each of four decoctions of Evodia rutaecarpa, recover the solvent with a rotary evaporator, add 20ml of 100% methanol to two of them, and add 20ml of acetonitrile-water (6:4) to the other two, accurately weigh the weight, and extract for 1 hour under reflux , take it out, add extraction solvent to make up the weight, filter, absorb 2ml, centrifuge at 16000r / min for 10min, and take the supernatant. The contents of the three components were determined, and the test results are shown in Table 2.
[0038] Table 2 The comparison of different extraction solvents to the main active ingredients in the test solution
[0039]
[0040] The results showed that when 100% methanol was used as the extraction solvent, the extraction effect was better than other solvents.
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