Method for regenerating adventitious buds by inducing callus tissue through cotyledons of herbaceous peony

Abstract

The invention relates to a method for regenerating adventitious buds by inducing callus tissue through cotyledons of a herbaceous peony. The method comprises the steps of: initiating cultivation by using herbaceous peony seed embryos, disinfecting seed embryos, cutting the embryos, inoculating the cut seed embryos to a basal culture medium of an MS solid medium, performing cultivation for 15 daysto obtain embryo seedlings, cultivating strong seedlings for 20-30 days to obtain tissue culture seedlings which grow robustly, and performing induced cultivation of the callus tissue for 45-60 days to obtain a callus tissue block which is plump, fresh and green and has a compact structure; and transplanting the callus tissue block into an adventitious bud differentiating culture medium to obtainthe adventurous buds which grow robustly. According to the method for regenerating the adventitious buds by inducing the callus tissue through the cotyledons of the herbaceous peony, the raw materialsare convenient to collect, the seed embryos are adopted as the tissue culture seedlings of explant culture, no limitation is caused by seasons, and the raw materials can be collected all the year; the contamination rate of first-generation embryo culture is far lower than that of other explants, and the induction rate of the callus tissue and the differentiating rate of the adventitious buds areimproved greatly; in addition, through the addition of an anti-browning agent in the phase of callus tissue induction and adventitious bud differentiation, browning can be inhibited effectively, and the problem of high contamination rate during induction of the callus tissue by the explants of the herbaceous peony can be solved.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for inducing callus to regenerate adventitious buds through peony cotyledons. Background technique [0002] Peony ( Paeonia lactiflora ) is a perennial root flower of Paeoniaceae Paeoniaceae. It has ornamental and medicinal value. It is a traditional famous flower in China. It has a long history of cultivation and is known as "flower phase". It is a commonly used plant for gardening. [0003] At present, traditional breeding methods such as artificial directional pollination, artificial mutagenesis and natural mutation are mostly used in the cultivation of new varieties of peony. Factors such as reproductive isolation limit the application of traditional breeding methods. For these reasons, the traditional breeding of peony takes a long time, the selection efficiency is low, and the renewal speed is slow, which makes it difficult for the current situatio...

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the above-mentioned deficiencies in the prior art, the present invention provides a method for inducing callus to regenerate adventitious buds through peony cotyledons, which can effectively solve the problem of high contamination rate of callus induced by peony explants and improve adventitious bud differentiation. rate, enhance the growth of buds, and is not affected by the season, and can be cultivated on an annual basis

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