Preparation method of natto kinase tablets
A technology of soybean kinase tablet and natto, which is applied to the functions of food ingredients, food ingredients as odor improvers, food heat treatment, etc., can solve the problems that are difficult to be popularized, and achieve high nattokinase activity and reasonable preparation methods , advanced technology and reliable effect
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[0022] Example 1
[0023] In this example, the implementation started with 10 kg of soybeans. Specifically, a method for preparing nattokinase tablets includes the following steps:
[0024] (1) Soak the soybeans for 18 hours and cook for 36 minutes at a pressure of 0.12MPa. After the cooking is completed, remove part of the liquid so that the weight ratio of soybeans to liquid is 1:1.2;
[0025] (2) Inoculate Bacillus subtilis under aseptic conditions at 85 degrees Celsius and ferment at 38 degrees Celsius for 22 hours;
[0026] (3) Add clear water to the natto fermentation mixture obtained by fermentation in step 2 until the weight ratio of natto to liquid is 1:5, then homogenize, then centrifuge and collect the supernatant;
[0027] (4) Perform ultrafiltration on the clear liquid obtained in step 3, and collect substances with an exclusion relative molecular weight of 10,000 or more to obtain an ultrafiltration concentrate;
[0028] (5) Use Sephadex G-50 column for column chromatograp...
Example Embodiment
[0030] Example 2
[0031] In this example, the implementation started with 10 kg of soybeans. Specifically, a method for preparing nattokinase tablets includes the following steps:
[0032] (1) Soak the soybeans for 20 hours, and cook for 40 minutes at a pressure of 0.10 MPa. After the cooking is completed, remove part of the liquid so that the weight ratio of soybeans to liquid is 1:1.5;
[0033] (2) Inoculate Bacillus subtilis under aseptic conditions at 80 degrees Celsius and ferment at 40 degrees Celsius for 20 hours;
[0034] (3) Add clear water to the natto fermentation mixture obtained by fermentation in step 2 until the weight ratio of natto to liquid is 1:4, then homogenize, then centrifuge and collect the supernatant;
[0035] (4) Perform ultrafiltration on the clear liquid obtained in step 3, and collect substances with an exclusion relative molecular weight of 10,000 or more to obtain an ultrafiltration concentrate;
[0036] (5) Use Sephadex G-50 column for column chromatogr...
Example Embodiment
[0038] Example 3
[0039] In this example, the implementation started with 10 kg of soybeans. Specifically, a method for preparing nattokinase tablets includes the following steps:
[0040] (1) Soak the soybeans for 15 hours and cook for 30 minutes at a pressure of 0.15 MPa. After the cooking is completed, remove part of the liquid so that the weight ratio of soybeans to liquid is 1:1.0;
[0041] (2) Inoculate Bacillus subtilis under aseptic conditions at 90 degrees Celsius and ferment for 24 hours at 37 degrees Celsius;
[0042] (3) Add clear water to the natto fermentation mixture obtained by fermentation in step 2 until the weight ratio of natto to liquid is 1:8, then homogenize, then centrifuge and collect the supernatant;
[0043] (4) Perform ultrafiltration on the clear liquid obtained in step 3, and collect substances with an exclusion relative molecular weight of 10,000 or more to obtain an ultrafiltration concentrate;
[0044] (5) Use Sephadex G-50 column for column chromatogra...
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