Single nucleotide polymorphism based primer set for hybrid identification of true and false peanuts and identification method thereof

Abstract

The invention relates to a single nucleotide polymorphism based primer set for hybrid identification of true and false peanuts and a method for performing hybrid identification on the true and false peanuts through the same primer set. With the adoption of the primer set and the method, 68 bites of up to 384 samples can be detected within one day, and the efficiency is increased 50-100 times by being compared with that of a microsatellite detection method. According to the primer set and the method, the single nucleotide polymorphism is directly read through the sequencing technique; the result is clear and reliable, and free from inter-sample interference problem.

Description

technical field [0001] The invention relates to a single nucleotide polymorphism peanut true and false hybrid identification primer set and method thereof. Background technique [0002] Peanut (Arachis hypogaea L.) is the fourth largest oil crop in the world and an important oil crop in the tropics and subtropics. It has been planted in more than 100 countries, and China is the largest peanut producer in the world. Peanut is a strictly self-pollinating crop. In the process of hybrid breeding, it is necessary to manually complete the whole process of female parent pollen detasseling, male parent pollen collection, and pollination. There are many uncontrollable factors in the process, resulting in a high probability of hybrids producing false hybrids. . Timely and accurate identification of true and false hybrids in the hybrid F1 generation is the key to the success of hybrid breeding. The traditional identification of true and false hybrids is mainly carried out by two meth...

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Problems solved by technology

Taking a hybrid combination as an example, 5%-10% polymorphism among peanut materials is calculated. This hybrid combination needs to screen 50-60 markers to obtain enough differential markers, and then use the screened markers to test the F1 hybrids. It takes two days to complete the whole experiment; ②The electrophoretic detection resolution of microsatellite markers is low, and the detection results are inaccurate. As a result, the heterozygous state cannot be accurately judged, resulting in a decrease in the accuracy of the identification results; ③Microsatellite markers Satellite markers have peak slippage during the PCR process, so the bands between allelic variations may overlap, making it impossible to accurately judge the electrophoresis results

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