Device and method for detecting mercury, lead and arsenic ions in water based on quantum dot energy resonance transfer of intelligent equipment
A technology of energy resonance transfer, intelligent equipment, applied in lead and arsenic ion devices, mercury field, can solve the problems of difficult to achieve on-site detection and analysis, complicated operation, complicated data processing and analysis, etc., achieve small workload, simple operation, process simple effect
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preparation example Construction
[0041] Preparation of glass fiber paper-based chips: use glass fiber filter paper with no background fluorescence as the glass fiber paper base, and build multiple channels on the paper base: the hydrophobic channel in the non-reaction zone and the hydrophilic channel in the reaction zone are arranged at intervals, and the preparation of the hydrophobic channel The method is: soak the glass fiber paper base in a mixture of 0.12 M methyltrichlorosilane MTS, toluene and hydrochloric acid, and react in a fume hood for 3-3.5 hours. After the reaction is complete, remove the glass fiber paper base and dry in the air To be used; the hydrophilic channel is a circular heavy metal detection reaction zone with an inner diameter of 2-6mm, and the glass fiber paper base in the reaction zone is not subjected to hydrophobic treatment;
[0042] B. The construction of the detection device: the glass fiber paper-based chip and the optical hardware device that provides uniform light for taking pict...
experiment example 1
[0044] Experimental example 1: Pb 2+ , Hg 2+ And As 3+ Single ion detection: using QD620-DNA2 fluorescent probe prepared by 32nM to detect Pb 2+ , 32nM prepared QD540-DNA1 fluorescent probe to detect Hg 2+ , 32nM prepared QD490-DNA3 fluorescent probe to detect As 3+ ; Use 10mM sodium dihydrogen phosphate / disodium hydrogen phosphate NaH2PO4 / Na2HPO4 to fix the above-mentioned fluorescent probes in the 3 reaction areas of the glass fiber paper-based chip; the concentration of Hg containing 0-1 µM 2+ , As 3+ Or contain 0-50 nM Pb at the concentration to be measured 2+ The aqueous solutions were added to the above-mentioned glass fiber paper-based chip reaction zone channels, and maintained at 25 ℃ for 0.5 h, so that Pb 2+ Or Hg 2+ The induced DNA configuration changes, making As 3+ The body chain is formed, the paired DNA chain is opened, and the reaction is completed. Then the smart device collects the RGB picture information of multiple different reaction areas of the glass fiber pap...
experiment example 2
[0045] Experimental example 2: Pb 2+ , Hg 2+ And As 3+ Detection of mixed solution: Use 10mM sodium dihydrogen phosphate / disodium hydrogen phosphate NaH2PO4 / Na2HPO4 to fix the fluorescent probes on the three reaction areas of the glass fiber paper-based chip; the Pb containing 0-1 µM concentration to be measured 2+ , Hg 2+ And As 3+ Was added to the above-mentioned glass fiber paper-based chip reaction zone channel, and maintained at 25 ℃ for 0.5 h to make Hg 2+ Induces the configuration flip of DNA1 to form T-Hg 2+ -T structure, Pb 2+ Induces conformational changes of DNA2 to form a G-quadruplex, As 3+ The body chain is formed to open the paired DNA chain and the reaction is completed. Then the smart device collects the RGB picture information of multiple different reaction areas of the glass fiber paper-based chip; the APP analysis program simultaneously obtains the fixed Pb 2+ ,Hg 2+ And As 3+ The R value, G value and B value corresponding to multiple pixels in the reaction area...
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