Application of electrochemical immunosensor in detection of multi-residue of sulfonamide medicines

A technology of sulfonamide drugs and immunosensor, which is applied in the field of food safety detection and analytical chemistry, can solve the problems of complex sample processing, inability to simultaneously detect multiple or high-throughput screening of a class of compounds, and achieve simple sample pretreatment, The effect of fast detection speed and low cost

Inactive Publication Date: 2018-04-27
南京师范大学淮安研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the detection methods of sulfonamides have developed rapidly, mainly including immunoassays such as enzyme-linked immunoassay, chemiluminescence enzyme-linked immunoassay, spectrophotometry, high performance liquid chromatography, liquid-mass spectrometry, etc., the above methods are currently A large number of applications are used to monitor the content of sulfonamides in food. The detection method is sensitive, the result is stable, and the reliability is high. However, most of them have the disadvantages of needing to use large instruments and complicated sample processing, and cannot achieve high-throughput detection of multiple or one type of compounds at the same time. filter

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  • Application of electrochemical immunosensor in detection of multi-residue of sulfonamide medicines
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  • Application of electrochemical immunosensor in detection of multi-residue of sulfonamide medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of electrochemical immunosensor

[0024] The glassy carbon electrode is pretreated and cleaned first, and then 6 µL of graphene-chitosan solution is drip-coated on the surface of the treated glassy carbon electrode, placed in a 37°C incubator to dry, and then 10 µL of sulfadiazine is drip-coated Put it on the electrode, dry it in an incubator at 37°C, and perform DPV detection respectively; finally insert the electrode into 500 microliters of 0.5% BSA solution and seal it for 40~50mim to obtain an electrochemical immunosensor modified by sulfadiazine / graphene-chitosan .

Embodiment 2

[0025] Example 2 Detection of sulfadiazine (SD)

[0026] Prepare the electrochemical immunosensor modified by sulfadiazine / graphene-chitosan by the method of Example 1, and immerse it in a total volume of 50 μL of PBS containing 8 μL of sulfadiazine antibody and a series of different concentrations of sulfadiazine standard solution Incubate in a 37°C incubator for 40 min in the incubation solution, wash with PBS after incubation, and then in 2 mmol / L K 3 [Fe(CN) 6 ] in the KCl solution (0.1mol / L) for DPV scanning, respectively record the peak current I at different concentrations, the DPV curve is as follows figure 1 As shown, the concentration of the graph from top to bottom is 1000 ng / mL, 300 ng / mL, 100 ng / mL, 30 ng / mL, 10 ng / mL, 3 ng / mL, 1 ng / mL, 0.3 ng / mL mL, 0.1 ng / mL, 0.03 ng / mL, and 0.01 ng / mL. When the concentration to be detected is defined as zero, the peak current value is I 0 , ΔI is the difference between the peak current of the detection sample and the peak c...

Embodiment 3

[0027] Example 3 Detection of sulfaguanidine (SG)

[0028] Prepare the electrochemical immunosensor modified by sulfadiazine / graphene-chitosan by the method of Example 1, and immerse it into the PBS containing 8 μL of sulfadiazine antibody and a series of different concentrations of sulfaguanidine standard solution with a total volume of 50 μL Incubate in a 37°C incubator for 40 min in the incubation solution, wash with PBS after incubation, and then in 2 mmol / L K 3 [Fe(CN) 6 ] in the KCl solution (0.1mol / L) for DPV scanning, respectively record the peak current I at different concentrations, the DPV curve is as follows image 3 As shown, the concentration of the graph from top to bottom is 1000 ng / mL, 300 ng / mL, 100 ng / mL, 30 ng / mL, 10 ng / mL, 3 ng / mL, 1 ng / mL, 0.3 ng / mL mL, 0.1 ng / mL, 0.03 ng / mL, and 0.01 ng / mL. When the concentration to be detected is defined as zero, the peak current value is I 0 , ΔI is the difference between the peak current of the detection sample an...

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Abstract

The invention discloses an application of an electrochemical immunosensor in detection of multi-residue of sulfonamide medicines. The application includes the steps of: physically modifying the surface of a glass-carbon electrode with a graphene-chitosan composite, and modifying the material with sulfadiazine through physical adsorption. On the basis of a detection method that a sulfadiazine antibody can be reacted with the sulfonamide medicine through a cross-immune reaction and indirect competitiveness is also employed, multi-residue detection on four sulfonamide medicines, including sulfadiazine, sulfaguanidine, sulfadimidine and sulfacetamide, detection limit being 0.006-0.1 ng / mL and linear range being 0.01-1000 ng / mL.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analytical chemistry, and in particular relates to the application of an electrochemical immunosensor modified by a sulfadiazine / graphene-chitosan complex in the detection of multiple residues of sulfa drugs. Background technique [0002] Sulfonamides (SAs) are a class of substances containing the structure of sulfanilamide, which can prevent and treat bacterial infections. Because of its low price and good antibacterial effect, it is widely used in animal breeding. At present, the abuse of sulfa drugs can enter the food chain through their accumulation in poultry and livestock tissues, affecting the health of consumers. The European Union, the United States and China have set the maximum limit standard for the total content of sulfonamides, and the edible muscle tissue of all animals should not exceed 100 μg / kg. [0003] In recent years, the detection methods of sulfonamides h...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 丁利霞赵波邵科峰王雅娟刘佳龙
Owner 南京师范大学淮安研究院
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